Highly sensitive biosensing of phenol based on the adsorption of the phenol enzymatic oxidation product on the surface of an electrochemically reduced graphene oxide-modified electrode

2018 ◽  
Vol 10 (23) ◽  
pp. 2731-2739 ◽  
Author(s):  
Amir Kaffash ◽  
Hamid R. Zare ◽  
Khosrow Rostami
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Enzymatic Oxidation ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Electrochemically Reduced Graphene Oxide ◽  
Phenol Determination ◽  
Peroxidase Enzyme

An electrochemically reduced graphene oxide and horseradish peroxidase enzyme modified electrode has been used for phenol determination.

scholarly journals Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Gen Liu ◽  
Wei Ma ◽  
Yan Luo ◽  
Deng-ming Sun ◽  
Shuang Shao
Keyword(s):  
Methylene Blue ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Composite Film ◽  
Simultaneous Determination ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

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