Synthesis of azonia cyanine derivatives as NIR fluorescent probes for nucleic acid detection and cell imaging

2019 ◽  
Vol 11 (28) ◽  
pp. 3523-3531
Author(s):  
Yuan Chen ◽  
Xue-Rui Wei ◽  
Ru Sun ◽  
Yu-Jie Xu ◽  
Jian-Jeng Ge
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Cell Imaging ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

A series of azonia cyanine dyes were designed and synthesized for nucleic acid detection and nucleolus imaging in fixed HeLa cells.

scholarly journals Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

BIOspektrum ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 624-627
Author(s):  
Ole Behrmann ◽  
Iris Bachmann ◽  
Frank Hufert ◽  
Gregory Dame
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Dna Polymerase ◽  
Fluorescent Probes ◽  
Reverse Transcription ◽  
Nucleic Acid Detection ◽  
Detection Scheme ◽  
Recombination Proteins ◽  
Target Dna ◽  
Rapid Amplification

Abstract The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

Boranil dye based “turn-on” fluorescent probes for detection of hydrogen peroxide and their cell imaging application

RSC Advances ◽  
2016 ◽  
Vol 6 (89) ◽  
pp. 85838-85843 ◽  
Author(s):  
Jayabalan Shanmugapriya ◽  
Kandasamy Rajaguru ◽  
Gandhi Sivaraman ◽  
Shanmugam Muthusubramanian ◽  
Nattamai Bhuvanesh
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Cell Imaging ◽  
Physiological Conditions ◽  
Turn On ◽  
Imaging Application

The synthesis of boranil dye fluorescent probes for the detection of hydrogen peroxide has been described. The probes have been successfully applied for imaging of H2O2 in HeLa cells under physiological conditions.

Preparation of monomethine cyanine dyes for nucleic acid detection

1998 ◽  
Vol 37 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Todor G Deligeorgiev ◽  
Daphinka A Zaneva ◽  
Seok Hong Kim ◽  
Ram W Sabnis
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Synthesis of novel monomeric and homodimeric cyanine dyes with thioacetyl substituents for nucleic acid detection

2007 ◽  
Vol 72 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Todor Deligeorgiev ◽  
Nikolai Gadjev ◽  
Aleksey Vasilev ◽  
Karl-Heinz Drexhage ◽  
S.M. Yarmoluk
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Intercalating Cyanine Dyes for Nucleic Acid Detection

2009 ◽  
Vol 2 (1) ◽  
pp. 1-26 ◽  
Author(s):  
Todor Deligeorgiev ◽  
Stefka Kaloyanova ◽  
Juan Vaquero
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Intercalating Cyanine Dyes for Nucleic Acid Detection

2010 ◽  
Vol 2 (1) ◽  
pp. 1-26 ◽  
Author(s):  
Todor G. Deligeorgiev ◽  
Stefka Kaloyanova ◽  
Juan J. Vaquero
Keyword(s):  
Nucleic Acid ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

Preparation of multicolor emitting carbon dots for HeLa cell imaging

2014 ◽  
Vol 38 (12) ◽  
pp. 6152-6160 ◽  
Author(s):  
Vaibhavkumar N. Mehta ◽  
Sanjay Jha ◽  
Rakesh Kumar Singhal ◽  
Suresh Kumar Kailasa
Keyword(s):  
Hela Cell ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Confocal Laser ◽  
Microscopic Images

Confocal laser microscopic images of HeLa cells using CDs as fluorescent probes.

Water-Soluble Tetraphenylethene Derivatives as Fluorescent “Light-Up” Probes for Nucleic Acid Detection and Their Applications in Cell Imaging

2013 ◽  
Vol 8 (8) ◽  
pp. 1806-1812 ◽  
Author(s):  
Yuning Hong ◽  
Sijie Chen ◽  
Chris Wai Tung Leung ◽  
Jacky Wing Yip Lam ◽  
Ben Zhong Tang
Keyword(s):  
Nucleic Acid ◽  
Cell Imaging ◽  
Water Soluble ◽  
Nucleic Acid Detection ◽  
Fluorescent Light

scholarly journals Interaction of cyanine dyes with nucleic acids. Meso-methylsubstituted trimethincyanines, as possible probes for fluorescent nucleic acid detection

2001 ◽  
Vol 17 (5) ◽  
pp. 448-454 ◽  
Author(s):  
S. S. Lukashov ◽  
I. E. Makovenko ◽  
M. Yu. Losytskyy ◽  
Yu. L. Slominskii ◽  
S. M. Yarmoluk
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection
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