Particokinetics and in vitro dose of high aspect ratio nanoparticles

Nanoscale ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 5209-5214 ◽  
Author(s):  
Seth Richard Price ◽  
Calum Kinnear ◽  
Sandor Balog
Keyword(s):  
Aspect Ratio ◽  
Cell Cultures ◽  
High Aspect Ratio ◽  
Adherent Cell

Adapting computational particokinetic models to address the dosage of high-aspect ratio nanomaterials for in vitro nanoparticle toxicology assays involving submerged adherent cell cultures.

Fabrication of high-aspect-ratio microstructures in polymer microfluid chips for in vitro single-cell analysis

2016 ◽  
Vol 61 (10) ◽  
pp. 1566-1571 ◽  
Author(s):  
A. S. Bukatin ◽  
I. S. Mukhin ◽  
E. I. Malyshev ◽  
I. V. Kukhtevich ◽  
A. A. Evstrapov ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Single Cell ◽  
High Aspect Ratio ◽  
Single Cell Analysis ◽  
Cell Analysis

scholarly journals High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

2019 ◽  
Vol 11 (31) ◽  
pp. 28125-28137 ◽  
Author(s):  
Gabriele Tullii ◽  
Federica Giona ◽  
Francesco Lodola ◽  
Silvio Bonfadini ◽  
Caterina Bossio ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Cell Cultures ◽  
High Aspect Ratio ◽  
Semiconducting Polymer ◽  
3D Cell Cultures

scholarly journals Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures

2014 ◽  
Vol 58 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Ewelina Iwan ◽  
Maria Szczotka ◽  
Jacek Kuźmak
Keyword(s):  
Cell Cultures ◽  
Nested Pcr ◽  
Leukemia Virus ◽  
Positive Control ◽  
Adherent Cell ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Pcr Method

Abstract The aim of the study was to develop an in situ PCR (IS-PCR) method for detection of bovine leukemia virus (BLV) in cell cultures. Samples from five BLV positive and five BLV negative cows were collected and dendritic cells (DCs) from blood, bone marrow, spleen, and lymph node were cultured. Cultures prepared from healthy animals were infected with BLV. After two weeks, the cells were tested by nested PCR and IS-PCR for the presence of proviral DNA. As a positive control adherent cell line permanently infected with BLV was used. BLV was successfully detected by IS-PCR in DCs from naturally infected cattle and DCs infected in vitro. In control, non-infected DCs, the results of the reaction were negative. The results of provirus detection by IS-PCR were similar with these performed with nested PCR. Additionally, IS-PCR provides many advantages, like specific localisation of infection and smaller number of cells needed as template for PCR.

scholarly journals Correction: High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro

2020 ◽  
Author(s):  
Xiao-Pei Li ◽  
Kai-Yun Qu ◽  
Feng Zhang ◽  
Han-Ning Jiang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Gold Nanowires ◽  
Cardiac Tissues ◽  
Dispersed Gold

Correction for ‘High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro’ by Xiao-Pei Li et al., J. Mater. Chem. B, 2020, 8, 7213–7224, DOI: 10.1039/D0TB00768D.

High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro

2020 ◽  
Vol 8 (32) ◽  
pp. 7213-7224 ◽  
Author(s):  
Xiao-Pei Li ◽  
Kai-Yun Qu ◽  
Feng Zhang ◽  
Han-Ning Jiang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Cardiac Tissue ◽  
Gold Nanowires ◽  
Tissue Formation ◽  
Cardiac Tissues ◽  
Cardiomyocyte Culture ◽  
Dispersed Gold

The prepared high-aspect-ratio water-dispersed gold nanowires are incorporated into GeIMA hydrogels for cardiomyocyte culture and micro-cardiac tissue formation.

scholarly journals PVP Assisted Synthesis of Hydroxyapatite Nanorods with Tunable Aspect Ratio and Bioactivity

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
A. Joseph Nathanael ◽  
Young Ho Seo ◽  
Tae Hwan Oh
Keyword(s):  
Electron Microscopy ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Xrd Analysis ◽  
Reaction Model ◽  
Morphological Variations ◽  
Viability Analysis ◽  
Selected Area Electron Diffraction ◽  
Length Width

Highly crystalline and reasonably uniform hydroxyapatite (HA) nanorods were prepared by polyvinylpyrrolidone (PVP) assisted hydrothermal synthesis which produces high aspect ratio (length/width) nanorods. The aspect ratio of the nanorods was higher in the presence of PVP and increased with increasing concentrations of PVP. X-ray diffraction (XRD) analysis showed that the HA nanorods were of the hexagonal apatite phase. High resolution transmission electron microscopy (HRTEM) and selected area electron diffraction (SAED) patterns confirmed that the nanorods grew in thec-axis direction (preferred orientation). The calculated lattice spacing was ~0.35 nm which is thec-axis value of hexagonal HA. Morphological variations of pristine and PVP added HA were evaluated by field emission scanning electron microscopy (FESEM) and TEM, which revealed that the presence of PVP greatly increased the aspect ratio of the HA nanorods. The formation mechanism of the PVP assisted HA was studied and a possible reaction model was given. Cell viability analysis byin vitrostudies showed encouraging results for the high aspect ratio nanorods and indicated a possibility for tuning the activity based on controlling the aspect ratio.

scholarly journals An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles

2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Carola Endes ◽  
Otmar Schmid ◽  
Calum Kinnear ◽  
Silvana Mueller ◽  
Sandra Camarero-Espinosa ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
In Vitro Testing ◽  
Testing Strategy

scholarly journals A 3-dimensional in vitro model of epithelioid granulomas induced by high aspect ratio nanomaterials

2011 ◽  
Vol 8 (1) ◽  
pp. 17 ◽  
Author(s):  
Vanesa C Sanchez ◽  
Paula Weston ◽  
Aihui Yan ◽  
Robert H Hurt ◽  
Agnes B Kane
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
In Vitro Model ◽  
3 Dimensional ◽  
Vitro Model

scholarly journals A high aspect ratio microelectrode array for mapping neural activity in vitro

2012 ◽  
Vol 204 (2) ◽  
pp. 296-305 ◽  
Author(s):  
Andrew B. Kibler ◽  
Brian G. Jamieson ◽  
Dominique M. Durand
Keyword(s):  
Aspect Ratio ◽  
Neural Activity ◽  
High Aspect Ratio ◽  
Microelectrode Array
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