scholarly journals Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

2021 ◽  
Author(s):  
Joke Elskens ◽  
Annemieke Madder
Keyword(s):  
Nucleic Acid ◽  
Target Identification ◽  
Oligonucleotide Probes ◽  
Biomarker Detection ◽  
Nucleic Acid Probes ◽  
Efficient Detection

Crosslinker-modified nucleic acid probes are promising substitutes for regular oligonucleotide probes in hybridization-based assays, as they allow a more selective and efficient detection of nucleic acid targets and nucleic acid biomarkers.

Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes.

2008 ◽  
Vol 55 (4) ◽  
pp. 713-720 ◽  
Author(s):  
Xianyu Piao ◽  
Liancheng Sun ◽  
Tianbiao Zhang ◽  
Youlin Gan ◽  
Yifu Guan
Keyword(s):  
Experimental Data ◽  
Nucleic Acid ◽  
Dna Microarray ◽  
Chemical Nature ◽  
Cooperative Behavior ◽  
Oligonucleotide Probes ◽  
Discrimination Accuracy ◽  
Nucleic Acid Probes

Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T approximately G:G > G:A > A:A approximately T:T > A:C approximately T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.

Syntheses and Characterization of Conjugates of Nucleic Acid Probes and 6-Aminoglucose-based Polymers

1997 ◽  
Vol 8 (5) ◽  
pp. 297-304 ◽  
Author(s):  
Thierry Delair ◽  
Bérengère Badey ◽  
Alain Domard ◽  
Christian Pichot ◽  
Bernard Mandrand
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Probes

scholarly journals Rapid and specific detection of Salmonella infections using chemically modified nucleic acid probes

2019 ◽  
Vol 1054 ◽  
pp. 157-166 ◽  
Author(s):  
Isabel Machado ◽  
Victoria Garrido ◽  
Luiza I. Hernandez ◽  
Juliana Botero ◽  
Nora Bastida ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Specific Detection ◽  
Nucleic Acid Probes ◽  
Chemically Modified ◽  
Salmonella Infections

Distinguishing L from M photopigment coding sequences by hybridization to novel locked nucleic acid (LNA) oligonucleotide probes

2008 ◽  
Vol 25 (3) ◽  
pp. 283-287
Author(s):  
CHRISTINA PETTAN-BREWER ◽  
LI FU ◽  
SAMIR S. DEEB
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Macaca Nemestrina ◽  
Oligonucleotide Probes ◽  
Coding Sequences ◽  
Cone Mosaic ◽  
Target Nucleic Acid ◽  
High Degree

Many attempts have been made over the years to distinguish human and primate L (long-wavelength sensitive) from M (middle-wavelength sensitive) cone photoreceptors using either immunohistochemistry or in situ hybridization. These attempts have been unsuccessful due to the very high degree of identity between the sequences of the L and M proteins and encoding mRNAs. The recent development of chemically modified oligonucleotide probes, referred to as locked nucleic acid (LNA) probes, has shown that they hybridize with much greater affinity and specificity to the target nucleic acid. This has greatly increased the potential for differentiating L from M cones by in situ hybridization. We have designed LNA oligonucleotide probes that are complementary to either the L or M coding sequences located in exon 5 of the Macaca nemestrina L and M pigment genes. We have shown that the LNA-M and LNA-L probes hybridize specifically to their respective target nucleic acid sequences in vitro. This result strongly suggests that these probes would be instrumental in rapidly distinguishing L from M cone in the entire retina, and in defining the cone mosaic during development and in adults.

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