Separation and purification of horseradish peroxidase from horseradish roots using a novel integrated method

The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification horseradish peroxidase (HRP) from...

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Separation and purification of foot-and-mouth disease virus by multiple-stage aqueous two-phase extraction system

Process Biochemistry ◽

10.1016/j.procbio.2018.11.002 ◽

2019 ◽

Vol 77 ◽

pp. 143-150 ◽

Cited By ~ 1

Author(s):

Ping Du ◽

Pu Sun ◽

Shiqi Sun ◽

Jinjie Dong ◽

Hu Dong ◽

...

Keyword(s):

Disease Virus ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Extraction System ◽

Phase Extraction ◽

Separation And Purification ◽

Two Phase ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Aqueous Two Phase Extraction

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Aqueous Two-Phase Extraction Combined with Chromatography: New Strategies for Preparative Separation and Purification of Capsaicin from Capsicum Oleoresin

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-014-1303-6 ◽

2014 ◽

Vol 175 (2) ◽

pp. 1018-1034 ◽

Cited By ~ 6

Author(s):

Pei-pei Zhao ◽

Yan-min Lu ◽

Cong-ping Tan ◽

Yan Liang ◽

Bo Cui

Keyword(s):

Phase Extraction ◽

Preparative Separation ◽

Separation And Purification ◽

Two Phase ◽

Aqueous Two Phase Extraction ◽

New Strategies

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Purification of recombinant protein A by aqueous two-phase extraction integrated with affinity precipitation

Biotechnology and Bioengineering ◽

10.1002/bit.260401112 ◽

1992 ◽

Vol 40 (11) ◽

pp. 1381-1387 ◽

Cited By ~ 58

Author(s):

Masamichi Kamihira ◽

Rajni Kaul ◽

Bo Mattiasson

Keyword(s):

Recombinant Protein ◽

Protein A ◽

Phase Extraction ◽

Two Phase ◽

Affinity Precipitation ◽

Aqueous Two Phase Extraction

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Integration of aqueous two-phase extraction and affinity precipitation for the purification of lactate dehydrogenase

Journal of Chromatography A ◽

10.1016/0021-9673(94)80103-7 ◽

1994 ◽

Vol 668 (1) ◽

pp. 145-152 ◽

Cited By ~ 33

Author(s):

Dong Guoqiang ◽

Rajni Kaul ◽

Bo Mattiasson

Keyword(s):

Lactate Dehydrogenase ◽

Phase Extraction ◽

Two Phase ◽

Affinity Precipitation ◽

Aqueous Two Phase Extraction

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Combination of aqueous two-phase extraction and cation-exchange chromatography: New strategies for separation and purification of alliin from garlic powder

Journal of Chromatography B ◽

10.1016/j.jchromb.2014.02.037 ◽

2014 ◽

Vol 957 ◽

pp. 60-67 ◽

Cited By ~ 11

Author(s):

Xiu-min Jiang ◽

Yan-min Lu ◽

Cong-ping Tan ◽

Yan -Liang ◽

Bo Cui

Keyword(s):

Cation Exchange ◽

Exchange Chromatography ◽

Phase Extraction ◽

Separation And Purification ◽

Cation Exchange Chromatography ◽

Two Phase ◽

Garlic Powder ◽

Aqueous Two Phase Extraction ◽

New Strategies

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Purification of trypsin by affinity precipitation and combining with aqueous two-phase extraction

Biotechnology Techniques ◽

10.1007/bf00159558 ◽

1995 ◽

Vol 9 (7) ◽

pp. 461-466 ◽

Cited By ~ 9

Author(s):

Jyh-Ping Chen ◽

Fong-Long Jang

Keyword(s):

Phase Extraction ◽

Two Phase ◽

Affinity Precipitation ◽

Aqueous Two Phase Extraction

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Separation and purification of laccases from two different fungi using aqueous two-phase extraction

Process Biochemistry ◽

10.1016/j.procbio.2013.11.006 ◽

2014 ◽

Vol 49 (2) ◽

pp. 335-346 ◽

Cited By ~ 30

Author(s):

Axel Prinz ◽

Jacqueline Hönig ◽

Ina Schüttmann ◽

Holger Zorn ◽

Tim Zeiner

Keyword(s):

Phase Extraction ◽

Separation And Purification ◽

Two Phase ◽

Aqueous Two Phase Extraction

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Study on Separation and Purification of Resveratrol in Wine Grape Residue with Aqueous Two Phase Extraction Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1743 ◽

2012 ◽

Vol 550-553 ◽

pp. 1743-1746 ◽

Cited By ~ 1

Author(s):

Ying Zi He ◽

Biao Wang ◽

Yan Zhuang ◽

Yu Ping Lu

Keyword(s):

Extraction Method ◽

Hplc Analysis ◽

Phase System ◽

Phase Extraction ◽

Wine Grape ◽

Separation And Purification ◽

Two Phase ◽

Organic Solvent Extraction ◽

Primary Separation ◽

Aqueous Two Phase Extraction

The separation and purification of resveratrol in wine grape residue with aqueous two phase extraction method were studied in this paper. Used identified ethanol - ammonium sulfate - water as the aqueous two-phase system, the wine grape residue extract substances separated well. By TLC and HPLC analysis, resveratrol content (13.3μg/ml) in aqueous two-phase extraction was higher than the results (2.342μg/ml) in organic solvent extraction. The results showed that the aqueous two phase extraction method could be used in primary separation and purification of resveratrol in wine grape residue.

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