scholarly journals Effect of contaminating proteins on the separation and purification of papain from papaya latex using aqueous two-phase extraction.

1990 ◽  
Vol 16 (4) ◽  
pp. 772-779 ◽  
Author(s):  
Ryoichi Kuboi ◽  
Wei-hong Wang ◽  
Isao Komasawa
1989 ◽  
Vol 15 (3) ◽  
pp. 497-503 ◽  
Author(s):  
Ryoichi Kuboi ◽  
Wei-Hong Wang ◽  
Katsuji Morimatsu ◽  
Setsuji Tone ◽  
Isao Komasawa

2021 ◽  
Author(s):  
Wei Ji ◽  
Wenmei Ao ◽  
Mengqiu Sun ◽  
Chunlai Feng ◽  
Yun Wang

The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification horseradish peroxidase (HRP) from...


2014 ◽  
Vol 49 (2) ◽  
pp. 335-346 ◽  
Author(s):  
Axel Prinz ◽  
Jacqueline Hönig ◽  
Ina Schüttmann ◽  
Holger Zorn ◽  
Tim Zeiner

2012 ◽  
Vol 550-553 ◽  
pp. 1743-1746 ◽  
Author(s):  
Ying Zi He ◽  
Biao Wang ◽  
Yan Zhuang ◽  
Yu Ping Lu

The separation and purification of resveratrol in wine grape residue with aqueous two phase extraction method were studied in this paper. Used identified ethanol - ammonium sulfate - water as the aqueous two-phase system, the wine grape residue extract substances separated well. By TLC and HPLC analysis, resveratrol content (13.3μg/ml) in aqueous two-phase extraction was higher than the results (2.342μg/ml) in organic solvent extraction. The results showed that the aqueous two phase extraction method could be used in primary separation and purification of resveratrol in wine grape residue.


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