Study on Separation and Purification of Resveratrol in Wine Grape Residue with Aqueous Two Phase Extraction Method

The separation and purification of resveratrol in wine grape residue with aqueous two phase extraction method were studied in this paper. Used identified ethanol - ammonium sulfate - water as the aqueous two-phase system, the wine grape residue extract substances separated well. By TLC and HPLC analysis, resveratrol content (13.3μg/ml) in aqueous two-phase extraction was higher than the results (2.342μg/ml) in organic solvent extraction. The results showed that the aqueous two phase extraction method could be used in primary separation and purification of resveratrol in wine grape residue.

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Separation and purification of horseradish peroxidase from horseradish roots using a novel integrated method

New Journal of Chemistry ◽

10.1039/d0nj04614k ◽

2021 ◽

Author(s):

Wei Ji ◽

Wenmei Ao ◽

Mengqiu Sun ◽

Chunlai Feng ◽

Yun Wang

Keyword(s):

Horseradish Peroxidase ◽

Phase Extraction ◽

Separation And Purification ◽

Two Phase ◽

Affinity Precipitation ◽

Integrated Method ◽

Novel Method ◽

Temperature Controlled ◽

Aqueous Two Phase Extraction

The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification horseradish peroxidase (HRP) from...

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Separation and purification of foot-and-mouth disease virus by multiple-stage aqueous two-phase extraction system

Process Biochemistry ◽

10.1016/j.procbio.2018.11.002 ◽

2019 ◽

Vol 77 ◽

pp. 143-150 ◽

Cited By ~ 1

Author(s):

Ping Du ◽

Pu Sun ◽

Shiqi Sun ◽

Jinjie Dong ◽

Hu Dong ◽

...

Keyword(s):

Disease Virus ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Extraction System ◽

Phase Extraction ◽

Separation And Purification ◽

Two Phase ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Aqueous Two Phase Extraction

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Aqueous Two-Phase Extraction of Potassium Glycyrrhizinate with Polyethylene Glycol/(NH4)2SO4

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1687 ◽

2012 ◽

Vol 550-553 ◽

pp. 1687-1690

Author(s):

Chen Sun ◽

Wen Ju Liu ◽

Hai Ming Zhang

Keyword(s):

Polyethylene Glycol ◽

Mass Fraction ◽

Ph Value ◽

Phase System ◽

Phase Extraction ◽

Two Phase ◽

Aqueous Two Phase System ◽

Result Show ◽

A New Technique ◽

Aqueous Two Phase Extraction

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

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