Spatial organization of biochemical cues in 3D-printed scaffolds to guide osteochondral tissue engineering

2021 ◽  
Vol 9 (20) ◽  
pp. 6813-6829
Author(s):  
Paula Camacho ◽  
Anne Behre ◽  
Matthew Fainor ◽  
Kelly B. Seims ◽  
Lesley W. Chow
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spatial Organization ◽  
3D Printed ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

Peptide-functionalized 3D-printed scaffolds drive mesenchymal stem cells (MSCs) differentiation towards osteogenesis or chondrogenesis based on the presence and organization of both cartilage-promoting and bone-promoting peptides.

scholarly journals Multi-Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co-Cultured Human Mesenchymal Stem Cells

2015 ◽  
Vol 15 (11) ◽  
pp. 1535-1545 ◽  
Author(s):  
Sofia Amadori ◽  
Paola Torricelli ◽  
Silvia Panzavolta ◽  
Annapaola Parrilli ◽  
Milena Fini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Response ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

Mesenchymal stem cells cultivated on scaffolds formed by 3D printed PCL matrices, coated with PLGA electrospun nanofibers for use in tissue engineering

2017 ◽  
Vol 3 (4) ◽  
pp. 045005 ◽  
Author(s):  
Natasha Maurmann ◽  
Daniela P Pereira ◽  
Daniela Burguez ◽  
Frederico D A de S Pereira ◽  
Paulo Inforçatti Neto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrospun Nanofibers ◽  
3D Printed

scholarly journals Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering

2015 ◽  
Vol 16 (4) ◽  
pp. 045001 ◽  
Author(s):  
Jian-Feng Pan ◽  
Shuo Li ◽  
Chang-An Guo ◽  
Du-Liang Xu ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Printed ◽  
Nanocomposite Scaffolds

Stem Cells in Osteochondral Tissue Engineering

2018 ◽  
pp. 359-372 ◽  
Author(s):  
Eleonora Pintus ◽  
Matteo Baldassarri ◽  
Luca Perazzo ◽  
Simone Natali ◽  
Diego Ghinelli ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

Regeneration of hyaline-like cartilage and subchondral bone simultaneously by poly(l-glutamic acid) based osteochondral scaffolds with induced autologous adipose derived stem cells

2016 ◽  
Vol 4 (15) ◽  
pp. 2628-2645 ◽  
Author(s):  
Kunxi Zhang ◽  
Shiming He ◽  
Shifeng Yan ◽  
Guifei Li ◽  
Danqing Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Glutamic Acid ◽  
Subchondral Bone ◽  
Hyaline Cartilage ◽  
Adipose Derived Stem Cells ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

Osteochondral tissue engineering is challenged by the difficulty in the regeneration of hyaline cartilage and the simultaneous regeneration of subchondral bone.

scholarly journals Mesenchymal stem cells loaded on 3D-printed gradient poly(ε-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Yanyan Cao ◽  
Peng Cheng ◽  
Shengbo Sang ◽  
Chuan Xiang ◽  
Yang An ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Morphology ◽  
Chondrogenic Differentiation ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Individual Layer ◽  
3D Printed

Abstract Cartilage has limited self-repair ability due to its avascular, alymphatic and aneural features. The combination of three-dimensional (3D) printing and tissue engineering provides an up-and-coming approach to address this issue. Here, we designed and fabricated a tri-layered (superficial layer (SL), middle layer (ML) and deep layer (DL)) stratified scaffold, inspired by the architecture of collagen fibers in native cartilage tissue. The scaffold was composed of 3D printed depth-dependent gradient poly(ε-caprolactone) (PCL) impregnated with methacrylated alginate (ALMA), and its morphological analysis and mechanical properties were tested. To prove the feasibility of the composite scaffolds for cartilage regeneration, the viability, proliferation, collagen deposition and chondrogenic differentiation of embedded rat bone marrow mesenchymal stem cells (BMSCs) in the scaffolds were assessed by Live/dead assay, CCK-8, DNA content, cell morphology, immunofluorescence and real-time reverse transcription polymerase chain reaction. BMSCs-loaded gradient PCL/ALMA scaffolds showed excellent cell survival, cell proliferation, cell morphology, collagen II deposition and hopeful chondrogenic differentiation compared with three individual-layer scaffolds. Hence, our study demonstrates the potential use of the gradient PCL/ALMA construct for enhanced cartilage tissue engineering.

Investigating the potential of human placenta-derived extracellular matrix sponges coupled with amniotic membrane-derived stem cells for osteochondral tissue engineering

2016 ◽  
Vol 4 (4) ◽  
pp. 613-625 ◽  
Author(s):  
Arun Prabhu Rameshbabu ◽  
Paulomi Ghosh ◽  
Elavarasan Subramani ◽  
Kamakshi Bankoti ◽  
Kausik Kapat ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
Human Placenta ◽  
Osteochondral Defects ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

Placental extracellular matrix for osteochondral defects.

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