scholarly journals STUDIES ON PROTEIN AND NUCLEIC ACID METABOLISM IN VIRUS-INFECTED MAMMALIAN CELLS. THE FORMATION OF A VIRUS-SPECIFIC ANTIGEN IN KREBS II ASCITES-TUMOUR CELLS INFECTED WITH ENCEPHALOMYOCARDITIS VIRUS

1965 ◽  
Vol 94 (2) ◽  
pp. 337-344 ◽  
Author(s):  
IM KERR ◽  
EM MARTIN ◽  
MG HAMILTON ◽  
TS WORK
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Acid Metabolism ◽  
Specific Antigen ◽  
Tumour Cells ◽  
Encephalomyocarditis Virus ◽  
Nucleic Acid Metabolism ◽  
Ascites Tumour ◽  
Ascites Tumour Cells

scholarly journals The pathway of glutamine and glutamate oxidation in isolated mitochondria from mammalian cells

1971 ◽  
Vol 125 (3) ◽  
pp. 757-763 ◽  
Author(s):  
Zoran Kovačević
Keyword(s):  
Glutamate Dehydrogenase ◽  
Mammalian Cells ◽  
Tumour Cells ◽  
Co2 Production ◽  
Isolated Mitochondria ◽  
Ascites Tumour ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Tumour ◽  
Ascites Tumour Cells ◽  
Ehrlich Ascites Tumour Cells

1. Pyruvate strongly inhibited aspartate production by mitochondria isolated from Ehrlich ascites-tumour cells, and rat kidney and liver respiring in the presence of glutamine or glutamate; the production of14CO2 from l-[U-14C]glutamine was not inhibited though that from l-[U-14C]glutamate was inhibited by more than 50%. 2. Inhibition of aspartate production during glutamine oxidation by intact Ehrlich ascites-tumour cells in the presence of glucose was not accompanied by inhibition of CO2 production. 3. The addition of amino-oxyacetate, which almost completely suppressed aspartate production, did not inhibit the respiration of the mitochondria in the presence of glutamine, though the respiration in the presence of glutamate was inhibited. 4. Glutamate stimulated the respiration of kidney mitochondria in the presence of glutamine, but the production of aspartate was the same as that in the presence of glutamate alone. 5. The results suggest that the oxidation of glutamate produced by the activity of mitochondrial glutaminase can proceed almost completely through the glutamate dehydrogenase pathway if the transamination pathway is inhibited. This indicates that the oxidation of glutamate is not limited by a high [NADPH]/[NADP+] ratio. 6. It is suggested that under physiological conditions the transamination pathway is a less favourable route for the oxidation of glutamate (produced by hydrolysis of glutamine) in Ehrlich ascites-tumour cells, and perhaps also kidney, than the glutamate dehydrogenase pathway, as the production of acetyl-CoA strongly inhibits the first mechanism. The predominance of the transamination pathway in the oxidation of glutamate by isolated mitochondria can be explained by a restricted permeability of the inner mitochondrial membrane to glutamate and by a more favourable location of glutamate–oxaloacetate transaminase compared with that of glutamate dehydrogenase.

Studies on the nucleic acid metabolism of renal tumour cells in vitro

1974 ◽  
Vol 2 (1) ◽  
Author(s):  
H. Takayasu ◽  
Y. Aso ◽  
K. Okada ◽  
Y. Hoshino ◽  
K. Koiso ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Tumour Cells ◽  
Renal Tumour ◽  
Nucleic Acid Metabolism

Changes in Nucleic Acid Metabolism of Tumour Cells under High Pressure Oxygen

Nature ◽  
1967 ◽  
Vol 215 (5108) ◽  
pp. 1378-1379 ◽  
Author(s):  
T. WATARI ◽  
T. HISHIZAWA ◽  
H. MATSUDAIRA
Keyword(s):  
High Pressure ◽  
Nucleic Acid ◽  
Acid Metabolism ◽  
Tumour Cells ◽  
Nucleic Acid Metabolism ◽  
Pressure Oxygen ◽  
High Pressure Oxygen
Sign in / Sign up

Export Citation Format

Share Document