scholarly journals The amino acid sequence around the reactive cysteine residue in human plasma Factor XIII (Short Communication)

1973 ◽  
Vol 135 (4) ◽  
pp. 901-903 ◽  
Author(s):  
J. John Holbrook ◽  
Rodney D. Cooke ◽  
I. Barry Kingston
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Plasma ◽  
Factor Xiii ◽  
Short Communication ◽  
Cysteine Residue ◽  
Α Subunit ◽  
Plasma Factor

1. Activated human plasma factor XIII was 65% inhibited by iodo[14C]acetate and incorporated 0.6 mol of label into the α subunit, which eventually was allowed to form a precipitate. 2. All the label was recovered as S-carboxymethylcysteine in a tetra-peptide of sequence Gly-Gln-Cys-Trp.

scholarly journals The Amino Acid Sequence of the A Protein (α Subunit) of the Tryptophan Synthetase of Escherichia coli

1967 ◽  
Vol 242 (22) ◽  
pp. 5442-5446 ◽  
Author(s):  
John R. Guest ◽  
Gabriel R. Drapeau ◽  
Bruce C. Carlton ◽  
Charles Yanofsky
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Α Subunit ◽  
Tryptophan Synthetase

Activation of Human Plasma Factor XIII by Thrombin

Thrombin ◽  
1992 ◽  
pp. 257-271 ◽  
Author(s):  
L. Lorand ◽  
J. T. Radek
Keyword(s):  
Human Plasma ◽  
Factor Xiii ◽  
Plasma Factor

ChemInform Abstract: AMINO-ACID SEQUENCE OF THE CB5(PHE) CYANOGEN BROMIDE FRAGMENT OF HUMAN PLASMA ALBUMIN

1976 ◽  
Vol 7 (10) ◽  
pp. no-no
Author(s):  
L. MORAVEK ◽  
V. KOSTKA ◽  
I. ROSENBERG ◽  
B. MELOUN ◽  
M. A. SABER
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Plasma ◽  
Cyanogen Bromide ◽  
Plasma Albumin ◽  
Cyanogen Bromide Fragment

scholarly journals The amino acid sequence of the α chain of the major haemoglobin of the rat (Rattus norvegicus)

1975 ◽  
Vol 149 (1) ◽  
pp. 259-269 ◽  
Author(s):  
C G Chua ◽  
R W Carrell ◽  
B H Howard
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Rattus Norvegicus ◽  
Peptide Mapping ◽  
Cysteine Residue ◽  
British Library ◽  
Peptide Fragments ◽  
Partial Amino Acid Sequence ◽  
Tryptic Peptides ◽  
Α Chain

1. A partial amino acid sequence of the α chain from the rat (Wistar, Rattus norvegicus) major haemoglobin is reported. The soluble tryptic peptides prepared from aminoethylated α-globin were separated by peptide ‘mapping’. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide fragments derived from secondary enzymic digestion. The insoluble ‘core’ peptides were further digested with chymotrypsin, thermolysin and pepsin to give smaller soluble peptides for sequencing. The tryptic peptides were ordered on the basis of their homology with the corresponding peptides of human α chain. 2. The proposed sequence is compared with that obtained by using an automated sequencer [Garrick et al. (1975) Biochem. J.149, 245-258]. The differences in sequence resulting from the two methods are discussed. 3. It is suggested that the externally situated cysteine (residue 13) is responsible for the observed inhibition of crystallization of rat haemoglobin at alkaline pH. 4. Detailed evidence for the sequence has been deposited as Supplementary Publication SUP 50047 (9 pages) at the British Library (Linding Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from which copies can be obtained on the terms given in Biochem. J. (1975) 145, 5.

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