scholarly journals Cholesterol feeding alters the metabolism of thoracic-duct lymph lipoprotein cholesterol in rabbits but not in rats

1973 ◽  
Vol 136 (1) ◽  
pp. 109-113 ◽  
Author(s):  
T. G. Redgrave
Keyword(s):  
Cholesteryl Ester ◽  
Intravenous Administration ◽  
Thoracic Duct ◽  
Dietary Cholesterol ◽  
Lipoprotein Cholesterol ◽  
Thoracic Duct Lymph ◽  
Cholesterol Feeding ◽  
Duct Lymph ◽  
Glyceryl Trioleate ◽  
Hydrolysis Of

1. Labelled thoracic-duct lymph was collected from rats and rabbits after test meals containing [14C]cholesterol and [2-3H]glyceryl trioleate. 2. The metabolism of labelled cholesterol and triglyceride was studied in normally fed and cholesterol-fed rats and rabbits injected with radioactive lymph from the same species. 3. In normally fed animals of both species, 10min after intravenous administration, about 80% of lymph cholesteryl ester but only about 10% of triglyceride was recovered in the liver after clearance from the plasma. This distribution is consistent with participation of ‘remnant’ particles in the metabolism of dietary lymph particles. 4. The metabolism of cleared lymph lipoprotein constituents was unchanged in cholesterol-fed rats, but the recovery of cholesteryl ester in the livers of the cholesterol-fed rabbits was decreased to 30% of the cleared dose. 5. The low recovery in cholesterol-fed rabbits was accounted for mainly by increased hydrolysis of cholesteryl ester. 6. It is proposed that differences between rats and rabbits in metabolism of dietary cholesterol might be partly due to the observed enhancement of hydrolysis of lymph lipoprotein cholesteryl ester in rabbits.

Chylomicron-lipid-splitting activity in homogenates of rabbit polymorphonuclear leukocytes

1965 ◽  
Vol 209 (4) ◽  
pp. 765-769 ◽  
Author(s):  
Peter Elsbach ◽  
Herbert J. Kayden
Keyword(s):  
Fatty Acid ◽  
Thoracic Duct ◽  
Polymorphonuclear Leukocytes ◽  
Total Activity ◽  
Thoracic Duct Lymph ◽  
Freezing And Thawing ◽  
Naturally Occurring ◽  
Duct Lymph ◽  
Repeated Freezing ◽  
Hydrolysis Of

Evidence for hydrolysis of chylomicron-triglyceride was sought in homogenates of polymorphonuclear leukocytes obtained from rabbit peritoneal exudates. Such activity was found using chylomicra from human thoracic duct lymph. Release of fatty acid was greatest at pH 4.8 and a triglyceride concentration of 5 mm. Most of the activity was found associated with particulate fractions of the homogenate. Almost 50% of the total activity occurred in association with the granules (lysosomes) of the leukocytes. Repeated freezing and thawing of whole homogenate or its particulate fractions resulted in an increase in activity of approximately 50%. The activity was inhibited by phosphate and NaF but not by protamine or iodoacetate. It is concluded that homogenates of leukocytes contain an acid glycerolester hydrolase, which splits triglycerides of naturally occurring lipoproteins. The intracellular distribution of the activity suggests that it is at least in part lysosomal in origin.

Diuretics, Hepatic and Thoracic Duct Lymph Flows in the Dog

1979 ◽  
Vol 56 (3) ◽  
pp. 211-214
Author(s):  
J. J. Szwed ◽  
D. R. Maxwell
Keyword(s):  
Intravenous Administration ◽  
Thoracic Duct ◽  
Ethacrynic Acid ◽  
Lymph Flow ◽  
Thoracic Duct Lymph ◽  
Diuretic Agent ◽  
Duct Lymph ◽  
Before And After

1. Simultaneous thoracic duct and hepatic lymph flows were measured in 29 mongrel dogs before and after the intravenous administration of mannitol, ethacrynic acid, frusemide and chlorothiazide in separate experiments. 2. Thoracic duct lymph flow increased significantly after each diuretic agent was administered. 3. Hepatic lymph flow increased only after ethacrynic acid and mannitol administration. Frusemide and chlorothiazide did not alter hepatic lymph flow. 4. These data show that increases in thoracic duct lymph flow after ethacrynic acid and mannitol arise partly from the liver, as well as from other organs.

Measurement of Pancreatic Enzymes in Human Thoracic Duct Lymph

1960 ◽  
Vol 38 (6) ◽  
pp. 954-956 ◽  
Author(s):  
Allan E. Dumont ◽  
John H. Mulholland
Keyword(s):  
Thoracic Duct ◽  
Pancreatic Enzymes ◽  
Thoracic Duct Lymph ◽  
Duct Lymph

Thoracic duct lymph. Relative contribution from splanchnic and muscle tissue

Diabetes ◽  
1993 ◽  
Vol 42 (5) ◽  
pp. 720-731 ◽  
Author(s):  
G. M. Steil ◽  
M. A. Meador ◽  
R. N. Bergman
Keyword(s):  
Muscle Tissue ◽  
Thoracic Duct ◽  
Thoracic Duct Lymph ◽  
Relative Contribution ◽  
Duct Lymph

Dynamics of glucose production and uptake are more closely related to insulin in hindlimb lymph than in thoracic duct lymph

Diabetes ◽  
1994 ◽  
Vol 43 (2) ◽  
pp. 180-190 ◽  
Author(s):  
R. A. Poulin ◽  
G. M. Steil ◽  
D. M. Moore ◽  
M. Ader ◽  
R. N. Bergman
Keyword(s):  
Thoracic Duct ◽  
Glucose Production ◽  
Thoracic Duct Lymph ◽  
Duct Lymph
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