Evidence against the proposed interaction of thionitrobenzoate with protein disulphide bonds
Thionitrobenzoate was prepared from 5,5′-dithiobis-(2-nitrobenzoic acid), and its reaction with several disulphide-containing proteins was examined under various conditions. No evidence for any cleavage of disulphide bonds was obtained. The claim by Robyt et al. [Arch. Biochem. Biophys. (1971) 147, 262–269] that thionitrobenzoate allows the quantitative determination of disulphide bonds in proteins is not substantiated.
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1989 ◽
Vol 62
(04)
◽
pp. 1043-1045
◽