Calcium ions and cell fusion. Effects of chemical fusogens on the permeability of erythrocytes to calcium and other ions

1. Fusogenic and non-fusogenic chemicals were tesetd for their ability to allow 45Ca2+ and 3H2O to enter hen and human erythrocytes. 2. The ratio of 45Ca2+/3H2O in treated cells to that in untreated cells is referred to as the entry ratio. 3. Within 1 min at 37 degrees C both water-soluble and lipid-soluble fusogens increased the value of the entry ratio, which reached maximum values in 5–10 min. 4. Values of the entry ratio in the range of 4–12 were found under conditions that led to cell fusion. 5. Closely related but non-fusogenic chemicals did not significantly alter the entry ratio. 6. The entry ratios for 86Rb+, 22Na+ and 35SO42- were also significantly increased by both lipid-soluble and water-soluble fusogens, though the increases were not as large as those for 45Ca2+. 7. It is suggested that fusogenic compounds increase the permeability of biological membranes to ions, and that an increase in the concentration of intracellular Ca2+ initiates or facilitates events that lead to the chemically induced fusion of erythrocytes.

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Magneto-sensitive hybrid nanocomposites of water-soluble sodium alginate cross-linked with calcium ions and maghemite

eXPRESS Polymer Letters ◽

10.3144/expresspolymlett.2018.38 ◽

2018 ◽

Vol 12 (5) ◽

pp. 452-461 ◽

Cited By ~ 2

Author(s):

V. V. Spiridonov ◽

I. G. Panova ◽

L. A. Makarova ◽

S. B. Zezin ◽

A. A. Novakova ◽

...

Keyword(s):

Sodium Alginate ◽

Calcium Ions ◽

Water Soluble ◽

Hybrid Nanocomposites

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Interaction of Sendai (HVJ) Virus with Human Erythrocytes: A Morphological Study of Haemolysis Cell Fusion

Journal of General Virology ◽

10.1099/0022-1317-15-3-227 ◽

1972 ◽

Vol 15 (3) ◽

pp. 227-234 ◽

Cited By ~ 58

Author(s):

K. Apostolov ◽

J. D. Almeida

Keyword(s):

Cell Fusion ◽

Morphological Study ◽

Human Erythrocytes

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Effects of calcium ions on proteolytic processing of HIV-1 gp160 precursor and on cell fusion

FEBS Letters ◽

10.1016/0014-5793(94)80284-x ◽

1994 ◽

Vol 338 (3) ◽

pp. 281-284 ◽

Cited By ~ 14

Author(s):

Maxime Moulard ◽

Luc Montagnier ◽

Elmostafa Bahraoui

Keyword(s):

Cell Fusion ◽

Calcium Ions ◽

Proteolytic Processing ◽

Hiv 1

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Studies on Chemically Induced Cell Fusion

Journal of Cell Science ◽

10.1242/jcs.10.3.769 ◽

1972 ◽

Vol 10 (3) ◽

pp. 769-787

Author(s):

Q. F. AHKONG ◽

F. C. CRAMP ◽

D. FISHER ◽

J. I. HOWELL ◽

J. A. LUCY

Keyword(s):

Aqueous Solution ◽

Cell Fusion ◽

Plasma Membranes ◽

Mouse Fibroblast ◽

Nuclear Membranes ◽

Multinucleated Cells ◽

Large Numbers ◽

Chemically Induced ◽

Complete Disintegration ◽

Unstable Plasma

Hen erythrocytes that were fixed after treatment with lysolecithin in aqueous solution for 30 s at 37 °C showed evidence of bridge formation between adjacent lysed cells. Generally, the homokaryons that were produced using lysolecithin in this way contained large numbers of nuclei. These giant syncytia had damaged nuclear membranes and unstable plasma membranes; complete disintegration of the syncytia occurred within 1 min of adding lysolecithin to the erythrocytes. In order to localize the action of lysolecithin, the fusing agent was incorporated into microdroplets of lipid. Cell fusion following the addition of lysolecithin in an aqueous glyceridelecithin emulsion was slower than with lysolecithin in aqueous solution, taking 10-30 min, and it was accompanied by considerably less damage to the plasma and nuclear membranes. The fused erythrocytes, which usually contained only two or three nuclei, lysed slowly during the 45 min following fusion, and lysis could be arrested by cooling the fused cells. The plasma membranes of lysed, multinucleated cells remained intact at 37°C for at least 90 h. Mouse fibroblast-hen erythrocyte heterokaryons formed with the aid of the emulsion were more stable than those produced with lysolecithin in solution, but the hybrid cells nevertheless had damaged subcellular organelles. Viable clones of hybrid mouse-hamster fibroblast cells were obtained using the emulsion although, possibly owing to reduced viability of the lysolecithin-treated cells, only at twice the frequency of spontaneously produced hybrids.

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