Mitochondrial thiol oxidase Erv1: both shuttle cysteine residues are required for its function with distinct roles

Erv1 is a sulfydryl oxidase, an essential component of mitochondrial MIA pathway. The present study shows that both shuttle cysteine residues of Erv1 are required for its function, they play complementary, but distinct, roles to ensure rapid turnover of active enzyme.

Download Full-text

Studies of mammalian ornithine decarboxylase using a monoclonal antibody

Biochemical Journal ◽

10.1042/bj2170123 ◽

1984 ◽

Vol 217 (1) ◽

pp. 123-128 ◽

Cited By ~ 10

Author(s):

A E Pegg ◽

J E Seely ◽

L Persson ◽

M Herlyn ◽

K Ponsell ◽

...

Keyword(s):

Monoclonal Antibody ◽

Ornithine Decarboxylase ◽

Mouse Kidney ◽

Immunoglobulin M ◽

Active Enzyme ◽

Sodium Thiocyanate ◽

Immunoaffinity Column ◽

Rapid Turnover ◽

Cytosolic Protein

A monoclonal antibody of the immunoglobulin M class was produced against mouse kidney ornithine decarboxylase. Screening for the antibody was carried out using alpha-difluoromethyl[5-3H]ornithine-labelled ornithine decarboxylase. The antibody reacted with this antigen and with native ornithine decarboxylase. The antibody attached to Sepharose could be used to form an immunoaffinity column that retained mammalian ornithine decarboxylase. The active enzyme could then be eluted in a highly purified form by 1.0M-sodium thiocyanate. The monoclonal antibody could also be used to precipitate labelled ornithine decarboxylase from homogenates of kidneys from androgen-treated mice given [35S]methionine. Only one band, corresponding to Mr of about 55000, was observed. The extensive labelling of this band is consistent with the rapid turnover of ornithine decarboxylase protein, since this enzyme represents only about 1 part in 10000 of the cytosolic protein.

Download Full-text

CRITICAL STUDY ON DATABASE AND PROMINENT PROGRAMMES

INTERNATIONAL JOURNAL OF COMPUTERS & TECHNOLOGY ◽

10.24297/ijct.v4i1a.3034 ◽

2013 ◽

Vol 4 (1) ◽

pp. 44-47

Author(s):

Dr. Vinod Kumar ◽

Gagandeep Raheja ◽

Sukhpreet Singh

Keyword(s):

Human Brain ◽

Everyday Life ◽

Modern Society ◽

Database Systems ◽

Simple Device ◽

Critical Study ◽

Essential Component ◽

The People

The people who work with computers, the programmers, analysts, and operators who seem to live by rules of their own and seldom leave their own environment, tend to be very cynical towards the stories of electronic brains. This attitude will appear hardly surprising when one eventually learns that the computer is a very simple device and is as far removed from an electronic brain as a bicycle from a spaceship. Programmers in particular are the people most aware that computers are no substitute for the human brain; in fact, the preparation of work to be run on a computer can be one of the most mind-bending exercises encountered in everyday life. Databases and database systems have become an essential component of everyday life in modern society. In the course of a day, most of us encounter several activities that involve some interaction with a database. So in this paper we will talk about how to manage the different type of data involved in any form in the database.

Download Full-text