Response of tissue diamine oxidase activity to polyamine administration

The administration to rats of putrescine (750 mumol/kg body wt.) caused in liver, kidney and heart an increase in putrescine at 1 h and in diamine oxidase (EC 1.4.3.6) activity within 3-6 h. An increase in spermidine was observed at 9 h in liver and at 6 h in kidney, whereas in heart there was no change. The increase in diamine oxidase activity by exogenous putrescine was prevented by the administration of actinomycin D and cycloheximide, suggesting that syntheses of mRNA and protein are involved. Equimolar doses of 1,3-diaminopropane, 1,5-diaminopentane and monoacetylputrescine stimulated, similarly to putrescine, hepatic, renal and cardiac diamine oxidase activity. After the injection of a non-toxic dose of spermidine (750 mumol/kg body wt.), the increase in diamine oxidase activity occurred at 9 h in all the tissues studied, when a substantial putrescine formation from spermidine occurred. sym-Norspermidine, which is unable to form putrescine, did not cause an increase in enzyme activity. The possibility that the tissue contents of putrescine might regulate diamine oxidase activity is discussed.

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Ornithine decarboxylase in rat small intestine: stimulation with food or insulin

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1976.231.5.1557 ◽

1976 ◽

Vol 231 (5) ◽

pp. 1557-1561 ◽

Cited By ~ 50

Author(s):

DV Maudsley ◽

J Leif ◽

Y Kobayashi

Keyword(s):

Enzyme Activity ◽

Small Intestine ◽

Ornithine Decarboxylase ◽

Diamine Oxidase ◽

Actinomycin D ◽

Histidine Decarboxylase ◽

Decarboxylase Activity ◽

Adenosylmethionine Decarboxylase ◽

Diamine Oxidase Activity ◽

Similarities And Differences

Ornithine decarboxylase in the small intestine of starved rats was stimulated 3- to 10-fold by refeeding or administration of insulin. A peak is observed 3-5 h following treatment after which the enzyme activity rapidly declines. The rise in ornithine decarboxylase is reduced by actinomycin D or cycloheximide. The increase in enzyme activity occurs mainly in the duodenum and jejunum with less than a twofold change being observed in the ileum. A small (twofold) increase in S-adenosylmethionine decarboxylase activity in the small intestine was observed after food, but there was no change in diamine oxidase activity. Whereas pentagastrin and metiamide administration markedly stimulated histidine decarbosylase in the gastric mucosa, no consistent effect of these agents on ornithine decarboxylase in the small intestine was observed. The similarities and differences between histidine decarboxylase and ornithine decarboxylase are discussed.

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Effect of cycloheximide and actinomycin D on estrogen-stimulated diamine oxidase activity in rat uterus

Pharmacological Research Communications ◽

10.1016/s0031-6989(88)80597-6 ◽

1988 ◽

Vol 20 ◽

pp. 181-182 ◽

Cited By ~ 1

Author(s):

Angela Sessa ◽

Antonio Perin

Keyword(s):

Oxidase Activity ◽

Diamine Oxidase ◽

Actinomycin D ◽

Rat Uterus ◽

Diamine Oxidase Activity

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Increased Diamine Oxidase Activity in the Intestinal Lymph of Rats on Fat Ingestion

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y74-099 ◽

1974 ◽

Vol 52 (3) ◽

pp. 760-762 ◽

Cited By ~ 11

Author(s):

A. Wollin ◽

L. B. Jaques

Keyword(s):

Enzyme Activity ◽

Olive Oil ◽

Intraperitoneal Injection ◽

Oxidase Activity ◽

Diamine Oxidase ◽

Glucose Solution ◽

Activity Levels ◽

Intestinal Lymph ◽

Combined Administration ◽

Diamine Oxidase Activity

When diamine oxidase activity was measured in the intestinal lymph of rats, a marked increase in the enzyme activity was observed shortly after oral administration of olive oil. The response was similar to that seen after intraperitoneal injection of heparin. Substitution of glucose solution for olive oil did not produce this response. Administration of olive oil on successive days resulted in a successively greater increase in diamine oxidase activity. Combined administration of olive oil and intraperitoneal injection of heparin gave diamine oxidase activity levels whch were greater than the sum of the increases resulting from the separate treatments.

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