Studies on fatty acid-binding proteins. The binding properties of rat liver fatty acid-binding protein
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1. The fluorescent fatty acid probe 11-(dansylamino)undecanoic acid binds to rat liver fatty acid-binding protein with a 1:1 stoichiometry. 2. The binding of the fluorescent probe is competitive with long-chain fatty acids. 3. Binding displacement studies were performed with a wide range of fatty acids and other ligands and identified C16 and C18 fatty acids as the preferred fatty acids for rat liver fatty acid-binding protein. No preference was observed for unsaturated fatty acids within this group. 4. Fatty acyl-CoA binds less well than the corresponding fatty acid.
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1989 ◽
Vol 264
(15)
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pp. 8708-8713
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1991 ◽
Vol 266
(9)
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pp. 5486-5496
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1990 ◽
Vol 265
(19)
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pp. 10955-10958
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1987 ◽
Vol 262
(12)
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pp. 5931-5937
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1986 ◽
Vol 879
(2)
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pp. 229-239
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