scholarly journals The effect of gabaculine on tetrapyrrole biosynthesis and heterotrophic growth in Cyanidium caldarium

1988 ◽  
Vol 254 (3) ◽  
pp. 907-910 ◽  
Author(s):  
J D Houghton ◽  
L Turner ◽  
S B Brown
Keyword(s):  
Chlorophyll Synthesis ◽  
Similar Rate ◽  
Red Alga ◽  
Heterotrophic Growth ◽  
Tetrapyrrole Biosynthesis ◽  
Cyanidium Caldarium ◽  
Pigment Synthesis ◽  
Subsequent Exposure ◽  
Light Inhibition ◽  
Lag Period

Pigment synthesis in four strains of the unicellular red alga Cyanidium caldarium with different pigment-synthesizing patterns was inhibited in the presence of gabaculine (3-amino-2,3-dihydrobenzoic acid). Parallel inhibition of light-induced chlorophyll and phycocyanin synthesis was observed in strain III-D-2, which only synthesizes pigments in the light. Similar parallel inhibition was observed in the dark in mutant CPD, which is able to synthesize chlorophyll and phycocyanin in the absence of light. Inhibition of pigment synthesis in all strains was overcome by addition of 5-aminolaevulinic acid. Inhibition of phycocyanin synthesis in mutant GGB (unable to synthesize chlorophyll) and inhibition of chlorophyll synthesis in mutant III-C (unable to synthesize phycocyanin) were also observed. Gabaculine also inhibited the heterotrophic growth of C. caldarium in the dark. However, inhibition was overcome after an extended lag period, following which cell growth proceeded at a similar rate to that of control cells not exposed to gabaculine. Heterotrophic growth in cells pre-exposed to gabaculine was not inhibited by subsequent exposure. Possible mechanisms for this adaptation are discussed.

scholarly journals REGULATORY ASPECTS OF NH4+ UTILIZATION IN THE ACIDOPHILIC THERMOPHILIC UNICELLULAR RED ALGA CYANIDIUM CALDARIUM

1987 ◽  
Vol 105 (2) ◽  
pp. 247-254 ◽  
Author(s):  
VITTORIA MARTINO RIGANO ◽  
VINCENZA VONA ◽  
CATELLO MARTINO ◽  
CARMELO RIGANO
Keyword(s):  
Red Alga ◽  
Cyanidium Caldarium ◽  
Regulatory Aspects

scholarly journals Biosynthesis of phycobiliproteins. Incorporation of biliverdin into phycocyanin of the red alga Cyanidium caldarium

1984 ◽  
Vol 219 (3) ◽  
pp. 905-909 ◽  
Author(s):  
S B Brown ◽  
J A Holroyd ◽  
D I Vernon
Keyword(s):  
Photosynthetic Pigments ◽  
Red Alga ◽  
Cyanidium Caldarium ◽  
Biliverdin Ix

14C-labelled biliverdin IX alpha was administered to cultures of Cyanidium caldarium that were actively synthesizing photosynthetic pigments in the light. Between 9 and 12% of the phycobiliprotein chromophore produced in such cultures was derived from exogenous biliverdin. These results demonstrate that biliverdin is an intermediate in the biosynthesis of phycobiliproteins.

scholarly journals Bile pigment synthesis in plants. Incorporation of haem into phycocyanobilin and phycobiliproteins in Cyanidium caldarium

1981 ◽  
Vol 194 (1) ◽  
pp. 137-147 ◽  
Author(s):  
S B Brown ◽  
J A Holroyd ◽  
R F Troxler ◽  
G D Offner
Keyword(s):  
Chlorophyll A ◽  
Protoporphyrin Ix ◽  
Bile Pigment ◽  
Cyanidium Caldarium ◽  
Pigment Synthesis ◽  
14C Labelling

A procedure was developed whereby haem was taken up by dark-grown cells of the unicellular rhodophyte Cyanidium caldarium. These cells were subsequently incubated either in the dark with 5-aminolaevulinate, which results in excretion of phycocyanobilin into the suspending medium or incubated in the light, which results in synthesis and accumulation of phycocyanin and chlorophyll a within the cells. Phycocyanobilin was isolated from phycocyanin by cleavage from apoprotein in methanol. Phycocyanobilin prepared from phycocyanin or excreted from cells given 5-aminolaevulinate was methylated and purified by t.l.c. By using 14C labelling either in the haem or in 5-aminolaevulinate administered, haem incorporation into phycocyanobilin was demonstrated in both dark and light systems. Since chlorophyll a synthesized in the light in the presence of labelled haem contained no radioactivity, it was clear that haem was directly incorporated into phycocyanobilin and not first converted into protoporphyrin IX. These results clearly demonstrate phycocyanobilin synthesis via haem and not via magnesium protoporphyrin IX as has also been postulated.

Effects of Chloramphenicol on Growth, Size Distribution, Chlorophyll Synthesis and Ultrastructure of Euglena Gracilis

1969 ◽  
Vol 4 (3) ◽  
pp. 627-644
Author(s):  
Y. BEN-SHAUL ◽  
Y. MARKUS
Keyword(s):  
Protein Synthesis ◽  
Cell Division ◽  
Cell Volume ◽  
Chlorophyll Synthesis ◽  
Secondary Effect ◽  
Mean Cell Volume ◽  
Initial Rise ◽  
Dividing Cells ◽  
Light Inhibition ◽  
The Mean

Multiplication of Euglena cells treated by 0.5-1.0 mg/ml chloramphenicol was not disturbed for the first 36 h and inhibition appeared only at later stages. The mean cell volume of treated dividing cells was decreased, although the initial rise in cell volume, which normally occurred during the first 12 h of incubation, was not prevented. The antibiotic also lowered the chlorophyll content of green dividing cells. In dard-grown cells transferred to light, inhibition of chlorophyll synthesis was immediate but not complete, and was followed by a decreased rate of plastid elongation and thylakoid formation. Our findings suggest that chloramphenicol does not cause the loss of existing pigment and that impaired chlorophyll synthesis is a secondary effect of inhibition of protein synthesis. The results also indicate that the greening process is more sensitive than cell division to the antibiotic.

Heterotrophic growth patterns in the unicellular alga Cyanidium caldarium

1977 ◽  
Vol 113 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Carmelo Rigano ◽  
Giovanni Aliotta ◽  
Vittoria Di Martino Rigano ◽  
Amodio Fuggi ◽  
Vincenza Vona
Keyword(s):  
Growth Patterns ◽  
Heterotrophic Growth ◽  
Unicellular Alga ◽  
Cyanidium Caldarium
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