scholarly journals Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180

1993 ◽  
Vol 291 (3) ◽  
pp. 951-951
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Cervix Carcinoma ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity ◽  
Human Cervix

scholarly journals Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180

1993 ◽  
Vol 289 (2) ◽  
pp. 357-361 ◽  
Author(s):  
G Werner-Felmayer ◽  
E R Werner ◽  
D Fuchs ◽  
A Hausen ◽  
B Mayer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
No Synthase ◽  
Carcinoma Cell Line ◽  
Ionophore A23187 ◽  
Cervix Carcinoma ◽  
Oxide Synthase ◽  
Human Cervix

We show here that the human cervix carcinoma cell line ME-180 expresses a constitutive nitric oxide (NO) synthase, as demonstrated by formation of [3H]citrulline and nitrite. The enzyme is dependent on tetrahydrobiopterin, NADPH, flavins and Ca2+/calmodulin. Enzyme activity is located in the cytosol rather than in the membrane fraction and can be inhibited by NG-monomethyl-L-arginine (NMMA). An antiserum to NO synthase purified from porcine cerebellum inhibited the enzyme activity. ME-180 cells released NO, as was shown by stimulation of guanylate cyclase (EC 4.6.1.2) in RFL-6 detector cells; this release was stimulated 8-fold by the Ca2+ ionophore A23187 and 2-fold by increasing the intracellular tetrahydrobiopterin levels with cytokines. This is the first characterization of a Ca2+/calmodulin-dependent NO synthase activity in human epithelial-type tumour cells.

Effect of KH-305 on the Nitric Oxide Synthase Activity and Erectile Dysfunction in Young Rats

2007 ◽  
Vol 36 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Eun-Jeong Lee ◽  
Hee-Seok Kim ◽  
Byoung-Chul Kim ◽  
Sung-Wan Hwang ◽  
Sung-Yeoun Hwang
Keyword(s):  
Nitric Oxide ◽  
Erectile Dysfunction ◽  
Nitric Oxide Synthase ◽  
Young Rats ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity
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