scholarly journals Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase

1993 ◽  
Vol 291 (3) ◽  
pp. 952-952
Keyword(s):  
Rat Liver ◽  
Immunochemical Characterization ◽  
Male Specific

scholarly journals Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase

1993 ◽  
Vol 289 (3) ◽  
pp. 719-725 ◽  
Author(s):  
E B Borthwick ◽  
A Burchell ◽  
M W Coughtrie
Keyword(s):  
Rat Liver ◽  
Human Liver ◽  
Regulatory Mechanism ◽  
Biologically Active ◽  
Male Rats ◽  
Immunochemical Characterization ◽  
Male Specific ◽  
The Brain ◽  
Brain Examination

Sulphation of oestrogens represents an important regulatory mechanism for these biologically active compounds. We have characterized and purified a form of rat liver sulphotransferase (ST), existing as a 32,500 Da monomer, which sulphates oestrogens, and have used this preparation to produce antibodies against oestrogen ST. The enzyme was active against oestrone, oestriol and beta-oestradiol, but not towards androgens. Using the antibody as a probe for immunoblotting, it was determined that the enzyme is expressed solely in male rats, and predominantly in the liver. Of the tissues examined, the only major extrahepatic tissue found to have any oestrogen ST was the brain (although the levels were very low), indicating that there might be a role for the sulphation of oestrogens in the brain. Examination of human liver and platelet cytosols by immunoblotting showed that the antibody recognized two major proteins of 32 and 34 kDa, which were presumed to correspond to the two principal phenol ST isoenzymes present in man.

Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions

1987 ◽  
Vol 142 (2) ◽  
pp. 367-375 ◽  
Author(s):  
Silvano Capitani ◽  
Peggy R. Girard ◽  
Gonzalo J. Mazzei ◽  
J.F. Kuo ◽  
Ronald Berezney ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Liver ◽  
Immunochemical Characterization ◽  
Rat Liver Nuclei ◽  
Kinase C ◽  
Liver Nuclei

Immunochemical characterization of cytochrome P-450 isozymes responsible for benzene oxidation in the rat liver

1989 ◽  
Vol 10 (9) ◽  
pp. 1713-1717 ◽  
Author(s):  
Tamie Nakajima ◽  
Eivor Elovaara ◽  
Sang S. Park ◽  
Harry V. Gelboin ◽  
Eino Hietanen ◽  
...  
Keyword(s):  
Rat Liver ◽  
Benzene Oxidation ◽  
Immunochemical Characterization ◽  
Cytochrome P 450

Purification and immunochemical characterization of the cytoplasmic androgen-binding protein of rat liver

Biochemistry ◽  
1989 ◽  
Vol 28 (4) ◽  
pp. 1732-1736 ◽  
Author(s):  
William F. Demyan ◽  
Fazlul H. Sarkar ◽  
C. V. Ramana Murty ◽  
Arun K. Roy
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Immunochemical Characterization ◽  
Androgen Binding Protein ◽  
Androgen Binding
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