EFFECT OF SUBSTRATE ON THE CATALYTIC ACTIVITY AND STABILITY OF NITRATE REDUCTASE FROM RHIZOBIUM MELILOTI

1981 ◽  
Vol 9 (2) ◽  
pp. 322P-322P
Author(s):  
Marianna Telegdi
Keyword(s):  
Catalytic Activity ◽  
Nitrate Reductase ◽  
Rhizobium Meliloti

Evidence for Common Genetic Determinants of Nitrogenase and Nitrate Reductase in Rhizobium meliloti

1973 ◽  
Vol 246 (153) ◽  
pp. 153-154 ◽  
Author(s):  
Á. KONDOROSI ◽  
ILONA BARABÁS ◽  
ZÓRA SVÁB ◽  
L. OROSZ ◽  
T. SIK ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Rhizobium Meliloti ◽  
Genetic Determinants

Effects of Vanadate on the Molybdoproteins Xanthine Oxidase and Nitrate Reductase: Kinetic Evidence for Multiple Site Interaction

1980 ◽  
Vol 35 (9-10) ◽  
pp. 702-707 ◽  
Author(s):  
Candadai S. Ramadoss
Keyword(s):  
Catalytic Activity ◽  
Nitrate Reductase ◽  
Xanthine Oxidase ◽  
Oxidase Activity ◽  
Metal Ion ◽  
Nadh Oxidase ◽  
Multiple Site ◽  
Vanadate Inhibition ◽  
Nadh Oxidase Activity

Abstract The inhibition of the activity of xanthine oxidase by vanadate was strikingly similar to vanadate inhibition of another molybdoprotein nitrate reductase. Although the main catalytic activity of both enzymes was inhibited, the partial NADH oxidase activity associated with these enzymes was stimulated several fold. It appears that the metal ion binds at multiple site in both enzymes. In the absence of any enzymes a combination of vanadium (V) and molybdenum (V) in air was found to oxide NADH rapidly.

scholarly journals ABSENCE OF A CORRELATION BETWEEN NITRATE REDUCTASE AND SYMBIOTIC NITROGEN FIXATION EFFICIENCY IN RHIZOBIUM MELILOTI

1980 ◽  
Vol 60 (1) ◽  
pp. 209-212 ◽  
Author(s):  
H. ANTOUN ◽  
L. M. BORDELEAU ◽  
D. PRÉVOST ◽  
R. A. LACHANCE
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Dry Matter ◽  
Symbiotic Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Specific Activities ◽  
Selection Of

Specific activities of the assimilatory and "regulatory" types of nitrate reductase were studied in 41 strains of Rhizobium meliloti having different symbiotic nitrogen fixation activities. Both nitrate reductase enzymes were present in very effective and ineffective strains and no significant correlation was found between the specific activities of the two enzymes and the dry matter yields of alfalfa obtained with the 41 strains. Measurements of the specific activities of the two nitrate reductase enzymes in the vegetative bacteria cannot be used as a rapid physiological test for the selection of very effective strains of R. meliloti.

Phenotypic reversion of nitrogenase in pleiotropic mutants of Rhizobium meliloti

1979 ◽  
Vol 25 (3) ◽  
pp. 298-301 ◽  
Author(s):  
Ilona Barabás ◽  
Tibor Sik
Keyword(s):  
Amino Acids ◽  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Symbiotic Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Amino Acid Utilization ◽  
Phenotypic Reversion

In two out of three pleiotropic mutants of Rhizobium meliloti, defective in nitrate reductase induced by amino acid utilization in vegetative bacteria and in symbiotic nitrogen fixation, nitrogenase activity could be restored completely by purines and partially by the amino acids L-glutamate, L-aspartate, L-glutamine, and L-asparagine. The compounds restoring effectiveness in nitrogen fixation did not restore nitrate reductase activity in vegetative bacteria. The restoration of effectiveness supports our earlier conclusion that the mutation is not in the structural gene for a suggested common subunit of nitrogenase and nitrate reductase.

Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants

Biochemistry ◽  
2010 ◽  
Vol 49 (11) ◽  
pp. 2424-2432 ◽  
Author(s):  
Vincent Fourmond ◽  
Bénédicte Burlat ◽  
Sébastien Dementin ◽  
Monique Sabaty ◽  
Pascal Arnoux ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Nitrate Reductase ◽  
Driving Force ◽  
Protein Film ◽  
Protein Film Voltammetry ◽  
Nitrate Reductase Mutants

Effect of Low Nitrate Supply on Nitrogen Fixation in Alfalfa Root Nodules Induced by Rhizobium meliloti Strains with Varied Nitrate Reductase Activity

1989 ◽  
Vol 135 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Cesáreo Arrese-Igor ◽  
José M. Estavillo ◽  
José I. Peña ◽  
Carmen Gonzalez-Murua ◽  
Pedro M. Aparicio-Tejo
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Rhizobium Meliloti ◽  
Reductase Activity ◽  
Root Nodules ◽  
Nitrate Supply ◽  
Alfalfa Root
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