Release of Colony-Stimulating Activity from the Isolated Perfused Rat Liver

1976 ◽  
Vol 50 (6) ◽  
pp. 539-544
Author(s):  
A. Howell ◽  
W. G. E. Cooksley ◽  
C. J. Danpure
Keyword(s):  
Rat Liver ◽  
Colony Formation ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Albumin Synthesis ◽  
Isolated Rat Liver ◽  
Colony Stimulating Activity ◽  
Isolated Rat

1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagle's medium and albumin. 2. Dialysable inhibitors of colony formation were also released during perfusions. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity.

scholarly journals Apoprotein-independent binding of chylomicron remnants to rat liver membranes

1991 ◽  
Vol 279 (3) ◽  
pp. 769-773 ◽  
Author(s):  
J Borensztajn ◽  
T J Kotlar ◽  
S Y Chang
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Differentiation ◽  
Direct Role ◽  
Isolated Rat Liver ◽  
Liver Membranes ◽  
Chylomicron Remnants ◽  
Isolated Rat

Rat lymph chylomicrons and chylomicron remnants were treated with trypsin or Pronase. The ability of the resulting apoprotein-free lipoproteins to be taken up by the isolated perfused rat liver, and to bind to isolated rat liver membranes, was examined. Compared with control lipoproteins, the apoprotein-free chylomicrons and remnants retained unaltered their capacity to be differentiated by the intact liver and by the isolated membranes. Further, control remnants and apoprotein-free remnants competed for binding to the isolated membranes. We conclude that apoproteins are not required for the hepatic differentiation between chylomicrons and remnants, and suggest that the lipoprotein phospholipids may play a direct role in this process.

Removal of Excess Cholesterol and Cholate by the Isolated Rat Liver From its Perfusate

1956 ◽  
Vol 184 (2) ◽  
pp. 412-414 ◽  
Author(s):  
Meyer Friedman ◽  
René Bine ◽  
Tad Ishida
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Isolated Rat Liver ◽  
Almost All ◽  
Isolated Liver ◽  
Isolated Rat

Perfusion of the isolated perfused rat liver with a perfusate containing hypercholesteremic and hypercholatemic blood results in the removal of some of the cholesterol and almost all of the excess cholate. The withdrawn cholesterol is deposited almost completely in the liver, whereas the withdrawn cholate is excreted promptly in the bile. It is concluded that the isolated liver behaves qualitatively similar to the liver of the intact rat in respect to cholesterol and cholate metabolism.

Effect of Varying Pco2 on Intracellular pH and Lactate Consumption in the Isolated Perfused Rat Liver

1978 ◽  
Vol 55 (2) ◽  
pp. 175-181 ◽  
Author(s):  
P. G. Baron ◽  
R. A. Iles ◽  
R. D. Cohen
Keyword(s):  
Intracellular Ph ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Lactate Metabolism ◽  
Isolated Rat Liver ◽  
Buffering Effect ◽  
Lactate Uptake ◽  
Lactate Consumption ◽  
Isolated Rat

1. The effects of varying Pco2 on lactate uptake and intracellular pH (pHl) were studied in the isolated rat liver perfused with differing concentrations of lactate. 2. In general, pHl and lactate uptake are inversely related to Pco2, and pHl and lactate uptake are directly related to each other, but the quantitative aspects and significance of these relationships vary with the availability of lactate. A model of hepatic lactate metabolism is proposed which may account for the quantitative variation. 3. The metabolism of lactate within the hepatocyte exerts a destabilizing effect on hepatocyte cell pH, in contrast to the buffering effect seen in predominantly glycolytic tissues. 4. An attempt is made to relate the findings to the disturbances of lactate metabolism in clinical respiratory failure.

Simultaneous perfusion of [4-14C]oestriol and [6,9-3H2]oestriol 16α-monoglucuronide through the isolated rat liver

1982 ◽  
Vol 100 (2) ◽  
pp. 274-278
Author(s):  
M. Höller ◽  
H. Weber ◽  
H. Breuer
Keyword(s):  
Rat Liver ◽  
Small Extent ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Perfusion Medium ◽  
Isolated Rat Liver ◽  
Isolated Rat

Abstract. The uptake of [4-14C]oestriol by the isolated perfused rat liver is 3.8 times faster as compared to that of simultaneously perfused [6,9-3H2]oestriol 16α-monoglucuronide. During perfusion the concentration of both radioactive oestrogens decreased exponentially in perfusion medium (apparent kel: 0.061 min−1 and 0.016 min−1, respectively). [6,9−3H2]Oestriol 16α-monoglucuronide was metabolized only to a small extent; more than 92% was secreted unchanged into the bile where it was highly concentrated (1800 nmol/g). In contrast [4-14C]oestriol was extensively metabolized; it was mainly hydroxylated at C-atom 2, leading to a rapid increase in the concentration of 2-hydroxyoestriol in the perfused medium. This metabolite was quickly taken up by the liver during recirculation and subsequently either methylated or sulphated. 2-Hydroxyoestriol monosulphate was glucuronated to 2-hydroxyoestriol 16α-monoglucuronide 3-sulphate, which was rapidly excreted into the bile. No double conjugate was formed when [6,9-3H2]oestriol 16α-monoglucuronide was perfused; this is additional evidence for the correctness of the assumption that monoglucuronides cannot serve as precursors of sulphoglucuronides.

FACTORS INFLUENCING THE NET CONSUMPTION OF GLUCOSE BY THE ISOLATED PERFUSED RAT LIVER

1965 ◽  
Vol 43 (4) ◽  
pp. 617-626 ◽  
Author(s):  
Ellen R. Gordon
Keyword(s):  
Steady State ◽  
Rat Liver ◽  
Glucose Consumption ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Steady State Condition ◽  
Isolated Rat Liver ◽  
Factors Influencing ◽  
Constant Rate ◽  
Isolated Rat

Several factors have been found to influence the ability of the isolated rat liver to achieve and maintain a steady-state condition when receiving glucose at a constant rate. The net glucose consumed could be altered by starving the experimental animal for 72 hours, by changes in the blood of donor rats produced by starvation, by lowering of the pH of the perfusate, and by injury to the liver itself. In cases in which the net glucose consumption by the liver was lowered by starvation of the donor rats for 24 hours, the addition of insulin to the perfusate doubled the net consumption of glucose. These experiments demonstrate that insulin has an effect on the net consumption of glucose by the liver.

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

1982 ◽  
Vol 47 (02) ◽  
pp. 166-172 ◽  
Author(s):  
Yoav Sharoni ◽  
Maria C Topal ◽  
Patricia R Tuttle ◽  
Henry Berger
Keyword(s):  
Rat Liver ◽  
Isoelectric Point ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Plasminogen Activators ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Molecular Weights ◽  
Physiological Relevance

SummaryOf the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Bone Marrow Colony Stimulating Activity from the Isolated Perfused Rat Liver

1974 ◽  
Vol 46 (2) ◽  
pp. 17P-17P
Author(s):  
A. Howell ◽  
W. G. E. Cooksley ◽  
C. J. Danpure
Keyword(s):  
Bone Marrow ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Colony Stimulating Activity
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