Use of Electron Spin Resonance Techniques for the Direct Detection of Free Radicals in Muscle Tissue during Experimental Skeletal Muscle Damage

1985 ◽  
Vol 69 (s12) ◽  
pp. 81P-82P
Author(s):  
M.J. Jackson ◽  
R.H.T Edwards ◽  
M.C.R. Symons
Keyword(s):  
Skeletal Muscle ◽  
Free Radicals ◽  
Electron Spin Resonance ◽  
Muscle Damage ◽  
Electron Spin ◽  
Muscle Tissue ◽  
Direct Detection ◽  
Skeletal Muscle Damage ◽  
Spin Resonance

Direct detection of circulating free radicals in the rats using electron spin resonance spectrometry

1992 ◽  
Vol 12 (2) ◽  
pp. 121-126 ◽  
Author(s):  
X WANG ◽  
J LIU ◽  
I YOKOI ◽  
M KOHNO ◽  
A MORI
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Direct Detection ◽  
Spin Resonance

A study of free radicals in paraffin embedded and deparaffinized human heart muscle tissue using electron spin resonance (ESR)

1987 ◽  
Vol 87 (5) ◽  
pp. 499-504 ◽  
Author(s):  
G. Elek ◽  
A. Rockenbauer ◽  
�. Magyar ◽  
L. J�kay
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Muscle Tissue ◽  
Heart Muscle ◽  
Human Heart ◽  
Spin Resonance

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

Flash-photolysis electron spin resonance in solution studies of free radicals

1984 ◽  
Vol 78 ◽  
pp. 257 ◽  
Author(s):  
Christopher D. Buckley ◽  
Andrew I. Grant ◽  
Keith A. McLauchlan ◽  
Andrew J. D. Ritchie
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Flash Photolysis ◽  
Solution Studies ◽  
Spin Resonance
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