Observations on the chemical nature of lead in human blood serum

1991 ◽  
Vol 81 (6) ◽  
pp. 823-829 ◽  
Author(s):  
A. J. A. Al-Modhefer ◽  
M. W. B. Bradbury ◽  
T. J. B. Simons

1. The binding of lead to human blood serum, and components of serum, was studied by titration with the addition of Pb(NO3)2 solution, monitoring the free Pb2+ concentration with a Pb2+ electrode, and by equilibrium dialysis. 2. In fresh serum, about 4999 out of 5000 parts of added lead were bound. This suggests that the free Pb2+ concentration is around 1/5000th of the total lead concentration in the serum of normal subjects, i.e. about 1 × 10−12 mol/l. 3. About 60% of the binding of lead in serum is abolished by standing in air, by dialysis or by treatment with N-ethylmaleimide. This appears to be due to the presence of thiol compounds, mainly cysteine. The remaining 40% appears to be due to protein, mainly albumin.

1994 ◽  
Vol 40 (6) ◽  
pp. 44-47
Author(s):  
A. A. Bulatov ◽  
A. G. Kiselyova ◽  
T. V. Gorshkova ◽  
N. B. Akopova

A radioimmunoassay test system has been designed for measurements of free a-subunit (AS) of glycoprotein hormones in human blood serum with a sensitivity of 0.15 ng/ml. This test system revealed the absence of a direct correlation between the levels of free AS and levels of glycoprotein hormones in the blood sera of women with various endocrine profile, this being indicative of the specificity of this test system, on the one hand, and, on the other, confirming the possibility of independent secretion by the pituitary of free AS into the blood. Basal blood serum level of free AS in normal subjects is low: 0,9 ng/ml in women aged 17 to 30. The level of free AS secretion in the blood of many patients with nonfunctioning tumors of the pituitary was found increased, this demonstrating the diagnostic significance of measuring free AS as a marker of such tumors.


1970 ◽  
Vol 24 (03/04) ◽  
pp. 334-337 ◽  
Author(s):  
R Honorato

Summary1. A technique to obtain human serum rich in factor V is described.2. Calcium increases the stability of factor V in the serum.


1926 ◽  
Vol 69 (1) ◽  
pp. 113-124
Author(s):  
Adolph Bernhard ◽  
Jacob J. Beaver

RSC Advances ◽  
2021 ◽  
Vol 11 (32) ◽  
pp. 19924-19934
Author(s):  
Pinky Sagar ◽  
Gopal Krishna Gupta ◽  
Monika Srivastava ◽  
Amit Srivastava ◽  
S. K. Srivastava

TE-CQDs synthesized via the hydrothermal method for the detection of Fe3+ in HBS.


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