scholarly journals Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

1999 ◽  
Vol 87 (3) ◽  
pp. 418-428 ◽  
Author(s):  
A. S. Waage ◽  
T. Vardund ◽  
V. Lund ◽  
G. Kapperud
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Environmental Water ◽  
Food Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Semi-nested polymerase chain reaction for detection of toxigenic Vibrio cholerae from environmental water samples

2007 ◽  
Vol 47 (3) ◽  
pp. 207-211 ◽  
Author(s):  
Ajay Kumar Goel ◽  
Shweta Bhadauria ◽  
Pramod Kumar ◽  
Dev V. Kamboj ◽  
Lokendra Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction Assay Coupled with Fluorescence Detection on Microwell Plates for Listeria monocytogenes in Foods

1995 ◽  
Vol 58 (6) ◽  
pp. 614-620 ◽  
Author(s):  
RAÚL J. CANO ◽  
DAWN M. NORTON ◽  
ALMA E. INZUNZA ◽  
JOSÉ GIL SÁNCHEZ ◽  
CHRISTIAN OSTE
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Fluorescence Detection ◽  
Polymerase Chain Reaction Assay ◽  
Food Samples ◽  
Fluorescence Measurement ◽  
Chain Reaction ◽  
Predictive Values ◽  
Dairy Food ◽  
Polymerase Chain

This study evaluates a polymerase chain-reaction assay coupled with fluorescence detection (PCR-FD) in microwell plates for Listeria monocytogenes in dairy and food samples. Guanidinium thiocyanate/silica–extracted cultures and milk and dairy-food samples were used as templates for a PCR assay in microwell plates, with a primer pair that amplifies a 221 bp segment of the internal transcribed spacer (ITS), in the presence of 0.5 μg/ml of ethidium bromide. Fluorescence of the PCR products was measured with a CytoFluor 2300 fluorescence measurement system (Millipore Corporation, Bedford, MA). The lowest level of detection of the assay was 10 to 100 CFU. A total of 326 food samples were tested, both by culture and by PCR-FD. The overall sensitivity of the PCR-FD assay was 95.2% and the specificity was 89.9%. Positive and negative predictive values were 74.8% and 99.4%, respectively. Based on the results obtained in this study it appears that the PCR-FD assay described here can be useful for screening a large number of milk and dairy-food samples for contamination by Listeria monocytogenes.

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