A two-stage continuous culture system to study the effect of supplemental alpha-lactalbumin and glycomacropeptide on mixed cultures of human gut bacteria challenged with enteropathogenic Escherichia coli and Salmonella serotype Typhimurium

2003 ◽  
Vol 95 (1) ◽  
pp. 44-53 ◽  
Author(s):  
W.M. Bruck ◽  
G. Graverholt ◽  
G.R. Gibson
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Culture System ◽  
Gut Bacteria ◽  
Mixed Cultures ◽  
Enteropathogenic Escherichia Coli ◽  
Human Gut ◽  
Two Stage ◽  
Continuous Culture System ◽  
Salmonella Serotype

Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

2014 ◽  
Vol 96 ◽  
pp. 111-118 ◽  
Author(s):  
David Feria-Gervasio ◽  
William Tottey ◽  
Nadia Gaci ◽  
Monique Alric ◽  
Jean-Michel Cardot ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Continuous Culture ◽  
Culture System ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Continuous Culture System

scholarly journals Colonization of Mucin by Human Intestinal Bacteria and Establishment of Biofilm Communities in a Two-Stage Continuous Culture System

2005 ◽  
Vol 71 (11) ◽  
pp. 7483-7492 ◽  
Author(s):  
Sandra Macfarlane ◽  
Emma J. Woodmansey ◽  
George T. Macfarlane
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Laser Scanning ◽  
Intestinal Bacteria ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Culture Methods ◽  
Two Stage ◽  
Bacterial Populations ◽  
Continuous Culture System

ABSTRACT The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus breakdown. The aims of this study were to determine how intestinal bacteria colonize mucus and to study physiologic and enzymatic factors involved in the destruction of this glycoprotein. Colonization of mucin gels by fecal bacteria was studied in vitro, using a two-stage continuous culture system, simulating conditions of nutrient availability and limitation characteristic of the proximal (vessel 1) and distal (vessel 2) colon. The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes. Gel samples were also taken for analysis of mucin-degrading enzymes and measurements of residual mucin sugars. Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia. Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.

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