A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells

2002 ◽  
Vol 208 (3) ◽  
pp. 158-166 ◽  
Author(s):  
A. Sawaguchi ◽  
K. L. Mcdonald ◽  
S. Karvar ◽  
J. G. Forte
Keyword(s):  
High Pressure ◽  
Fine Structure ◽  
Primary Culture ◽  
Parietal Cells ◽  
High Pressure Freezing ◽  
New Approach ◽  
Culture Cells ◽  
Gastric Parietal Cells

The fine structure of human gastric parietal cells

1965 ◽  
Vol 10 (1) ◽  
pp. 13-21 ◽  
Author(s):  
G. Victor Rohrer ◽  
J. Randolph Scott ◽  
Walter Joel ◽  
Stewart Wolf
Keyword(s):  
Fine Structure ◽  
Parietal Cells ◽  
Gastric Parietal Cells

A new approach for cryofixation by high-pressure freezing

2001 ◽  
Vol 203 (3) ◽  
pp. 285-294 ◽  
Author(s):  
D. Studer ◽  
W. Graber ◽  
A. Al-Amoudi ◽  
P. Eggli
Keyword(s):  
High Pressure ◽  
High Pressure Freezing ◽  
New Approach

scholarly journals Integrating Light and TEM Information with F-TEM images

2005 ◽  
Vol 13 (5) ◽  
pp. 16-19
Author(s):  
P.A. Sims ◽  
C.A. Lockwood ◽  
J.D. Hardin
Keyword(s):  
Tissue Culture ◽  
High Pressure ◽  
Light Microscopy ◽  
Fusion Proteins ◽  
Freeze Substitution ◽  
High Pressure Freezing ◽  
Thin Sections ◽  
Embedding Method ◽  
Culture Cells ◽  
Tissue Culture Cells

Fluorescent fusion proteins are widely used to visualize the localization of proteins in worms, fish, flies and tissue culture cells. We have used two different methods that use high pressure freezing (HPF) combined with correlative light microscopy (LM) and TEM. The first method uses fluorescence from live organisms immobilized in agarose followed by HPF and standard freeze substitution in dry solvent with osmium. This pre-embedding method optimizes ultrastructural preservation. A second, post-embedding method preserves fluorescence and immunoreactivity from embedded and polymerized thin sections. Here we describe post-embedding fluorescent integrated TEM images (F-TEM).

Fine structure of hippocampal mossy fiber synapses following rapid high-pressure freezing

Epilepsia ◽  
2012 ◽  
Vol 53 ◽  
pp. 4-8 ◽  
Author(s):  
Shanting Zhao ◽  
Daniel Studer ◽  
Werner Graber ◽  
Sigrun Nestel ◽  
Michael Frotscher
Keyword(s):  
High Pressure ◽  
Fine Structure ◽  
Mossy Fiber ◽  
High Pressure Freezing

scholarly journals Preservation of Immunoreactivity and Fine Structure of AdultC. elegansTissues Using High-pressure Freezing

2004 ◽  
Vol 52 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Philippe Rostaing ◽  
Robby M. Weimer ◽  
Erik M. Jorgensen ◽  
Antoine Triller ◽  
Jean-Louis Bessereau
Keyword(s):  
High Pressure ◽  
Fine Structure ◽  
High Pressure Freezing
Sign in / Sign up

Export Citation Format

Share Document