Screening of Mhc variation in Atlantic salmon (Salmo salar): a comparison of restriction fragment length polymorphism (RFLP), denaturing gradient gel electrophoresis (DGGE) and sequencing

2000 ◽  
Vol 9 (2) ◽  
pp. 215-219 ◽  
Author(s):  
Asa Langefors ◽  
Jakob Lohm ◽  
Torbjorn von Schantz ◽  
Mats Grahn
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis ◽  
Restriction Fragment Length

scholarly journals Optimization of Terminal-Restriction Fragment Length Polymorphism Analysis for Complex Marine Bacterioplankton Communities and Comparison with Denaturing Gradient Gel Electrophoresis

1999 ◽  
Vol 65 (8) ◽  
pp. 3518-3525 ◽  
Author(s):  
Markus M. Moeseneder ◽  
Jesús M. Arrieta ◽  
Gerard Muyzer ◽  
Christian Winter ◽  
Gerhard J. Herndl
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Length Polymorphism ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Marine Bacterioplankton ◽  
Gradient Gel Electrophoresis ◽  
Restriction Fragment Length ◽  
Bacterioplankton Communities

ABSTRACT The potential of terminal-restriction fragment length polymorphism (T-RFLP) and the detection of operational taxonomic units (OTUs) by capillary electrophoresis (CE) to characterize marine bacterioplankton communities was compared with that of denaturing gradient gel electrophoresis (DGGE). A protocol has been developed to optimize the separation and detection of OTUs between 20 and 1,632 bp by using CE and laser-induced fluorescence detection. Additionally, we compared T-RFLP fingerprinting to DGGE optimized for detection of less abundant OTUs. Similar results were obtained with both fingerprinting techniques, although the T-RFLP approach and CE detection of OTUs was more sensitive, as indicated by the higher number of OTUs detected. We tested the T-RFLP fingerprinting technique on complex marine bacterial communities by using the 16S rRNA gene and 16S rRNA as templates for PCR. Samples from the Northern and Middle Adriatic Sea and from the South and North Aegean Sea were compared. Distinct clusters were identifiable for different sampling sites. Thus, this technique is useful for rapid evaluation of the biogeographical distribution and relationships of bacterioplankton communities.

scholarly journals Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

2000 ◽  
Vol 38 (11) ◽  
pp. 4249-4253 ◽  
Author(s):  
Pallavi Garg ◽  
Ranjan K. Nandy ◽  
Papiya Chaudhury ◽  
Nandini Roy Chowdhury ◽  
Keya De ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Vibrio Cholerae ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Pulsed Field Gel Electrophoresis ◽  
Vibrio Cholerae O1 ◽  
El Tor ◽  
Restriction Fragment Length

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular characterization by ribotyping, restriction fragment length polymorphism, and pulsed-field gel electrophoresis indicated that recent Inaba strains are remarkably different from the earlier Inaba strains but are very similar to the prevailing V. choleraeO1 Ogawa El Tor biotype strains. The antibiograms of the Inaba strains were also similar to those of the recent V. cholerae Ogawa strains. These V. cholerae O1 Inaba strains appear to have evolved from the currently prevailing Ogawa strains and are likely to dominate in the coming years.

scholarly journals Identification of Smoked Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss) Using PCR-Restriction Fragment Length Polymorphism of the p53 Gene

2000 ◽  
Vol 83 (2) ◽  
pp. 341-346 ◽  
Author(s):  
Esther Carrera ◽  
Teresa García ◽  
Ana Céspedes ◽  
Isabel González ◽  
Alicia Fernández ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Restriction Fragment Length Polymorphism ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
P53 Gene ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Pcr Products ◽  
Restriction Fragment Length

Abstract Raw and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the p53 gene. DNA from S. salar and O. mykiss was amplified by using primers flanking exons 5 to 6 of the p53 nuclear gene. PCR products of different length were obtained for each species (532 and 518 base pairs, respectively). Sequences of PCR products obtained from S. salar and O. mykiss were compared in the search for polymorphic restriction sites. The restriction fragments obtained with Eco RV, Hinf I, and Taq I endonucleases showed interspecific polymorphism, making it a useful method for identification of Atlantic salmon and rainbow trout.

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