Random amplified polymorphic DNA variation within and among meadow bromegrass progeny types

Studies of genetic variation at the DNA level in the tree fruit and nut crop species of Prunus have been very limited. Recently molecular markers based on random amplified polymorphic DNA (RAPD) markers have been shown to be highly useful and efficient gene markers in other plant and animal species. We have used a total of 50 primers (10-mers) with arbitrary nucleotide sequence to identify cultivars of cherry, plum, apricot, peach and almond. A total of 120 accessions of different cultivars were assayed. The variation revealed by RAPD markers was highly species specific in the five Prunus species examined. High levels of polymorphism were observed for almond cultivars whereas sweet cherry revealed the lowest levels of polymorphism for the RAPD primers examined. The implications of these results in the germplasm diversity in the cultivated species of Prunus will be discussed.

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Random amplified polymorphic DNA variation in the eggplant, Solanum melongena L. (Solanaceae)

Theoretical and Applied Genetics ◽

10.1007/bf00222010 ◽

1995 ◽

Vol 90 (6) ◽

pp. 767-770 ◽

Cited By ~ 44

Author(s):

J. L. Karihaloo ◽

S. Brauner ◽

L. D. Gottlieb

Keyword(s):

Random Amplified Polymorphic Dna ◽

Solanum Melongena ◽

Dna Variation

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Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

Progressive Agriculture ◽

10.3329/pa.v20i1-2.16839 ◽

2013 ◽

Vol 20 (1-2) ◽

pp. 1-8

Author(s):

MM Rahman ◽

L Rahman ◽

SN Begum ◽

F Nur

Keyword(s):

Genetic Distance ◽

Gene Diversity ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Molecular Genetic Analysis ◽

Polymorphic Loci ◽

Rice Genotypes ◽

High Level ◽

Genetic Diversity Study ◽

Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Neis genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 8, 2009

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EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

FLORA AND FAUNA ◽

10.33451/florafauna.v23i2pp461-467 ◽

2017 ◽

Vol 23 (2) ◽

Cited By ~ 1

Author(s):

SUNITA BORDE ◽

ASAWARI FARTADE ◽

AMOL THOSAR ◽

RAHUL KHAWAL

Keyword(s):

Fresh Water ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Band Pattern ◽

Genomic Variation ◽

Polymorphic Band ◽

Rapd Profile ◽

Band Size ◽

Pcr Product ◽

The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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