Ammonia-oxidizing bacterial communities are affected by nitrogen fertilization and grass species in native C4 grassland soils

Background Fertilizer addition can contribute to nitrogen (N) losses from soil by affecting microbial populations responsible for nitrification. However, the effects of N fertilization on ammonia oxidizing bacteria under C4 perennial grasses in nutrient-poor grasslands are not well studied. Methods In this study, a field experiment was used to assess the effects of N fertilization rate (0, 67, and 202 kg N ha−1) and grass species (switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii)) on ammonia-oxidizing bacterial (AOB) communities in C4 grassland soils using quantitative PCR, quantitative reverse transcription-PCR, and high-throughput amplicon sequencing of amoA genes. Results Nitrosospira were dominant AOB in the C4 grassland soil throughout the growing season. N fertilization rate had a stronger influence on AOB community composition than C4 grass species. Elevated N fertilizer application increased the abundance, activity, and alpha-diversity of AOB communities as well as nitrification potential, nitrous oxide (N2O) emission and soil acidity. The abundance and species richness of AOB were higher under switchgrass compared to big bluestem. Soil pH, nitrate, nitrification potential, and N2O emission were significantly related to the variability in AOB community structures (p < 0.05).

RECLAMATION OF MARGINAL LANDS USING RARE ENERGY CROPS

The purpose of the paper is to determine the impact of the species of energy crops on biomass yields and the possibility of their involvement in the reclamation of contaminated areas. This is especially important from the point of view of the rational use of land for energy crops cultivation. Methodology. The research object is the processes of growth and development of plants, the peculiarities of the yield formation of energy crops biomass depending on the species traits and growing conditions. The research subject is the following energy crops: Big Bluestem, Indiangrass and Columbus Grass as well as the plant biometric indicators, biomass yield and energy efficiency of biomass production of energy crops (2016-2020). The results of research showed the variability of biometric parameters of energy crops. Over the research years, the dry biomass yield of Indiangrass was 8.9 t/ha in the first year, 10.1 t/ha in the second year and 14.9 t/ha in the third year, Big Bluestem – varied within 4.4–9.3 t/ha. Columbus Grass dry biomass increased from 11.4 t/ha (1st year) to 14.9 t/ha (2nd year) to 18.0 t/ha (3rd year). The developed model for the creation of artificial phytocenoses will allow land reclamation using energy crops based on agroecological monitoring and justification when growing energy crops. Perennial cultivation of Columbus Grass and Indiangrass provided the highest coefficient of energy efficiency (at a level or more than 3.0), which is typical for average efficiency of biomass production. Therefore, Indiangrass and Columbus Grass are recommended to be grown in order to reclaim marginal lands and obtain sustainable plant raw materials. Big Bluestem is recommended to be grown only as a companion crop of stand of grass. Furthermore, energy crops must be cultivated on the basis of ecological and adaptive technology elements, taking into account the defined territorial conditions. For the conditions of Ukraine, this complex will make it possible to reduce the negative impact on the environment as well as to obtain the stable yields of various biomass for its further processing and energy conversion.

Evaluating Digestibility and Toxicity of Native Warm-season Grasses for Equines

Introduced cool-season grasses are dominant in Virginia’s grasslands, but their high digestible energy and non-structural carbohydrate (NSC) levels pose a risk for horses prone to obesity and laminitis. Native warm-season grasses (NWSG) have lower digestible energy and NSC levels that may be more suitable for horses susceptible to laminitis. Although NWSGs have desirable characteristics, they are novel forages for horses. Little is known about NWSG intake or potential toxicity to horses or how grazing by horses may affect NWSG swards. The overall objectives of this research were to 1) assess voluntary intake, toxicological response, and apparent digestibility of NWSG hays fed to horses; and 2) evaluate the characteristics of three NWSG species under equine grazing. For the first objective, a hay feeding trial using indiangrass (IG) (Sorghastrum nutans) and big bluestem (BB) (Andropogon gerardii) was conducted with 9 Thoroughbred geldings in a replicated 3 x 3 Latin square design. Voluntary dry matter intake of IG and BB hays by horses were 1.3% and 1.1% of BW/d, lower than orchardgrass (Dactylis glomerata), an introduced cool-season grass, at 1.7% of BW/d (P = 0.0020). Biomarkers for hepatotoxicity remained within acceptable ranges for all treatments. Apparent dry matter digestibility (DMD) did not differ among hays, ranging from 39 to 43%. Non-structural carbohydrate levels ranged from 4.4 to 5.4%, below maximum recommended concentrations for horses susceptible to laminitis. For the second objective, a grazing trial was conducted comparing IG, BB, and eastern gamagrass (Tripsacum dactyloides) (EG) yields, forage losses, changes in vegetative composition, and effects on equine bodyweight. Nine, 0.1-hectare plots were seeded with one of the three native grass treatments, and each plot was grazed by one Thoroughbred gelding in two grazing bouts, one in July and another in September 2019. Indiangrass had the greatest available forage, at 4340 kg/ha, compared with 3590 kg/ha from BB (P &lt; 0.0001). Eastern gamagrass plots established poorly, and had only 650 kg/ha available forage during the experiment. Grazing reduced standing cover of native grasses in IG and BB treatments by about 30%. Horses lost 0.5 to 1.5 kg BW/d on all treatments. Findings suggest indiangrass and big bluestem merit further consideration as forages for horses susceptible to obesity and pasture-associated laminitis.

20 Comparison of native warm-season grasses and bermudagrass for stocker calf grazing in the Black Belt Prairie region

Native grasses are touted for use in drought mitigation strategies for grazing cattle. It is important to determine how these forages compare in specific production environments to more widely used improved grasses such as bermudagrass [Cynodon dactylon (L.) Pers.] that they may replace as pasture. Native warm-season grasses including big bluestem (Andropogon gerardi Vitman), little bluestem (Andropogon scoparius), and indiangrass (Sorghastrum nutans L.) were evaluated against bermudagrass pastures in the Black Belt Prairie region of Mississippi for stocker cattle grazing. Objectives were to compare bermudagrass (BG), indiangrass (IG) and mixed-sward native warm-season grass (mix of big bluestem, little bluestem, and indiangrass) (NGMIX) pasture for forage nutritive value and steer ADG. Crossbred (Bos taurus) steers (n = 36 steers/year) were stratified by initial BW (339.7 ± 4.3 kg) to 2.02-ha pastures (3 replications) during 56-d grazing periods in June and July for 2 yr. Mean forage nutritive values on a DM basis were: BG [8.8% CP, 43.4% ADF, 67.3% NDF, 58.7% TDN, and 77 relative feed value (RFV)], IG (6.7% CP, 39.6% ADF, 68.3% NDF, 58.2% TDN, and 79 RFV) and MIXNG (7.3% CP, 40.5% ADF, 69.5% NDF, 58.1% TDN, and 77 RFV). A forage treatment x day x year effect existed (P &lt; 0.01) for each these nutritive values. There was no effect (P = 0.47) of forage treatment on steer ADG (BG: 0.4 ± 0.1 kg/d; IG: 0.5 ± 0.1 kg/d; NGMIX: 0.5 ± 0.1 kg/d). Thus, no net advantage or disadvantage in steer growth rates was observed due to forage species. Other factors such as forage establishment cost and animal stocking rates supported may be relevant to consider in decisions to replace bermudagrass with warm-season native grasses.

Water-Use Efficiency of Forage Crops in the Southeastern United States

Preparing agricultural producers to cope with volatile weather changes, specifically drought, requires a better understanding of forage water-use efficiency (WUE) potentials. Options to improve farm resiliency to drought may include the use of C4 annual and perennial forages, which have greater production efficiency during drought than commonly used C3 forages. Our objective was to measure WUE through real-time gas exchange measurements of photosynthesis and transpiration in (1) a greenhouse study and (2) under field-grazing conditions. Growth parameters, instantaneous water use efficiency (iWUE), and mass-based WUE (mWUE) data were collected under greenhouse conditions in Study 1 for the following species: crabgrass (Digitaria sanguinalis cv. ‘Red River’), switchgrass (Panicum virgatum cv. ‘Alamo’), big bluestem (Andropogon gerardii cv. ‘OZ-70’), indiangrass (Sorghastum nutans cv. ‘Rumsey’), eastern gamagrass (Tripsacum dactyloides cv. ‘Pete’), bermudagrass (Cynodon dactylon cv. ‘Vaughn’s #1’), sorghum-sudangrass (Sorghum bicolor (L.) × Sorghum sudanese (P.) cv. ‘Greengrazer’), and tall fescue (Schedonorus arundinaceus (Schreb.) Dumort). Study 2 occurred from 2014 to 2016, and evaluated iWUE of crabgrass, switchgrass, bermudagrass, eastern gamagrass, and a big bluestem/indiangrass mix under field conditions. Overall, in situ iWUE of crabgrass, switchgrass, eastern gamagrass, and bermudagrass did not differ, while iWUE of the big bluestem/indiangrass was less than switchgrass and crabgrass, an advantage for these species if the standardized precipitation index drops below zero. Bermudagrass, switchgrass, sorghum-sudangrass, pearl millet, and indiangrass had comparable mWUE values under greenhouse-simulated drought. These results will aid in the development of forage species recommendations for mitigating drought and improving resiliency.

Plant Diversity and Nitrogen Addition on Belowground Biodiversity and Soil Organic Carbon Storage in Biofuel Cropping Systems

Bioenergy production may reduce the emission of CO2 which contributes to climate change, particularly when management strategies are adopted that promote soil carbon (C) sequestration in bioenergy cropping systems. Planting perennial native grasses, such as switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerardii Vitman) may be used as a strategy to enhance soil C accumulation owing to their extensive root systems. Fertilizer use may further promote soil C sequestration, because of its positive impacts on plant production and soil C input. However, the influence of fertilizer addition on soil C accumulation is variable across bioenergy cropping systems, and fertilizer can negatively impact the environment. Increasing plant diversity may be used as a strategy to enhance soil C accumulation while augmenting other ecosystem properties such as soil biodiversity. The present study evaluates how inter- and intra- specific plant community diversity and N addition influence soil C storage and soil biodiversity. Soil was collected from a long-term (9 growing seasons) field experiment located at the Fermilab National Environmental Research Park in Illinois, USA. Treatments included [1] three cultivars of big bluestem and three cultivars of switchgrass cultivars grown in monoculture, [2] plant community diversity manipulated at both the species- and cultivar level, and [3] nitrogen (N) applied annually at two levels (0 and 67 kg ha-1). The soil at the site was dominated by C3 grasses for 30 years before replacement with C4 bioenergy grasses, which enabled quantification of plant-derived C accumulation owing to the natural difference in isotopic signature between C3 and C4 grasses. Soil samples were analyzed for [1] soil C and its δ13C isotopic signature, and [2] nematode and soil bacterial diversity. Our results indicate that both plant diversity and N addition influence soil community structure but not soil C storage or soil nematode biodiversity. However, the addition of big bluestem to the plant species mixes enhanced plant-derived C storage. In summary, our findings suggest that plant species identity can control soil C accumulation in the years following land conversion, and that manipulating plant community structure in bioenergy cropping systems may have a greater positive impact on soil C accumulation than N fertilization.

Alternative Hosts in the Families Poaceae and Cyperaceae for Xanthomonas vasicola pv. vasculorum, Causal Agent of Bacterial Leaf Streak of Corn

The bacterial pathogen Xanthomonas vasicola pv. vasculorum was first reported in the United States causing bacterial leaf streak on Nebraska corn (Zea mays) in 2016. The bacterium is also known to cause disease in sugarcane, grain sorghum, broom bamboo, and various palm species. The objective of this study was to identify alternative hosts for X. vasicola pv. vasculorum among plants commonly found in corn growing areas of the United States. In repeated greenhouse experiments, 53 species of plants found in the United States that had not been tested previously for susceptibility to X. vasicola pv. vasculorum were inoculated with the pathogen and monitored for symptom development. Eleven species in the family Poaceae exhibited symptoms: oat (Avena sativa), rice (Oryza sativa), orchardgrass (Dactylis glomerata), indiangrass (Sorghastrum nutans), big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), timothy (Phleum pratense), sand bluestem (Andropogon hallii), green foxtail (Setaria viridis), bristly foxtail (Setaria verticillata), and johnsongrass (Sorghum halepense). Yellow nutsedge (Cyperus esculentus) in the Cyperaceae also was a symptomatic host. In addition, endophytic colonization by X. vasicola pv. vasculorum was found in three asymptomatic alternative hosts: downy brome (Bromus tectorum), tall fescue (Festuca arundinacea), and western wheatgrass (Pascopyum smithii). Experiments were also conducted in the field to determine the potential for alternative hosts to become infected by natural inoculum. Symptoms developed only in big bluestem and bristly foxtail in field experiments. These results suggest that infection of alternative hosts by X. vasicola pv. vasculorum can occur, but infection rates might be limited by environmental conditions.