A new Epidendrum species of the Nocturnum group is proposed for the Brazilian central Amazon, near Manaus. It is described, illustrated, and compared with similar species. Epidendrum dayseae can be recognized by the pendent stem, the long, narrow leaves, a relatively long floral pedicel, and the lateral lobes of the lip smaller than the mid-lobe and deeply separated. The new species resembles E. longicolle, but is distinguished by the union between of the lateral and mid-lobes of the lip being under half the mid-lobe length. The new species is also compared with E. plurifolionocturnum. Its chromosome number is 2n = 4x = 80, with a band pattern similar to other species of the Nocturnum group.
Abstract
Mangrove is a very typical ecosystem with a high level of diversity of flora and fauna. However, mangrove ecosystems are also one of the most vulnerable locations to extinction due to the high disturbances that occur. Some of the species that are susceptible to such disturbances are fish and shrimp. The use of barcode DNA is considered a fairly effective way to evaluate the condition of fish and shrimp populations in North Sumatra. Therefore, this study aims to obtain information on the success of kit methods and amplification patterns of barcoding marks 16S for fish and shrimp in mangrove ecosystems North Sumatra. The results showed that the kit extraction method produced a pattern of band that varied thick, thin and even smear. PCR amplification results with the 16S gene showed a good band pattern and indicated that the 16S primer used can detect fish and shrimp species that are visualized in the form of DNA bands.
Abstract
This study aimed to examine the appearance of the DNA band pattern resulted from the selection of RAPD primers on Phaius spp. namely Phaius tankervillae, Phaius montanus, Phaius collasus and Phaius amboinensis. The research material was performed in the Center for Plant Conservation of the LIPI Bogor Botanical Gardens. Molecular analysis was carried out at the Laboratory of the Center for Horticultural and Tropical Studies IPB using RAPD. The study showed that the 2 primers RAPD OPA 02 and OPA 16 can be used for DNA amplification of orchids Phaius spp (Phaius tankervillae, Phaius montanus Phaius collasus and Phaius amboinensis) because they produce clear DNA bands. The result of PCR amplification on Phaius tankervillae, Phaius montanus, Phaius collasus, and Phaius amboinensis using OPA 02 and OPA 16 primers produced 11 and 9 DNA bands, respectively, with an average of 5 DNA bands per primer. In the band pattern at 800 bp on OPA 02 primers resulting sharp and clear band pattern quality.
Isolation and genomic amplification of fish and shrimps from mangrove ecosystems in North Sumatra