scholarly journals Detection of Brucella melitensis by the BacT/Alert automated system and Brucella broth culture

2002 ◽  
Vol 8 (11) ◽  
pp. 749-752 ◽  
Author(s):  
Z. Özkurt ◽  
S. Erol ◽  
M.A. Tasyaran ◽  
A. Kaya
1999 ◽  
Vol 37 (5) ◽  
pp. 1602-1605 ◽  
Author(s):  
F. Zuhre Badak ◽  
Servet Goksel ◽  
Ruchan Sertoz ◽  
Bedii Nafile ◽  
Safak Ermertcan ◽  
...  

The feasibility of using nucleic acid probes directly from positive MB/BacT broth to identify mycobacteria was determined in this study. A total number of 2,727 specimens were cultured into the MB/BacT (Organon Teknika) automated system and on conventional Loweinstein-Jensen (LJ) slants. The Gen-Probe AccuProbe culture identification tests (DNA probes) were used on samples from bottles which were identified as positive for mycobacteria by MB/BacT. Samples of positive MB/BacT broth (0.1 ml) were used directly in the broth culture method for the DNA probes as published by Gen-Probe. Centrifugation of the contents of the bottle was not done prior to probe testing. The number of mycobacteria detected by MB/BacT and LJ was 253 (221 isolates of M. tuberculosis and 32 isolates of mycobacteria other than M. tuberculosis [MOTT]). A total of 96.4% (213 of 221) of the bottles growing M. tuberculosis produced a positive direct DNA probe result for M. tuberculosis complex. One hundred percent (16 of 16) of the bottles growing M. gordonaeproduced a positive direct DNA probe result for M. gordonae. A total of 3.6% (8 of 221) of the bottles growingM. tuberculosis did not yield a positive direct DNA probe result for M. tuberculosis complex. The testing of subcultures made onto solid media from the positive bottles by AccuProbe identified six of these eight M. tuberculosisisolates. Two (0.9%) M. tuberculosis isolates gave a negative result for the M. tuberculosis probe test applied on the MB/BacT broth and its subculture. The rest of the positive MB/BacT bottles growing MOTT (16 of 32) were negative for M. gordonae, M. avium, M. intracellulare, and M. kansasii probes. The sensitivity and specificity of AccuProbe for the identification of M. tuberculosis andM. gordonae directly from MB/BacT broth were 96.4 and 100% for M. tuberculosis and 100 and 100% for M. gordonae, respectively. The direct testing of positive MB/BacT broth by AccuProbe, without prior centrifugation, allows for the accurate and rapid identification of M. tuberculosis andM. gordonae.


1974 ◽  
Author(s):  
Peter H. Henry ◽  
Roy A. Turner ◽  
Robert B. Matthie

2020 ◽  
Vol 6 (2) ◽  
pp. 147-153
Author(s):  
Muhamad Yusup ◽  
Po. Abas Sunarya ◽  
Krisandi Aprilyanto

System The process of counting and storing in a manual water reservoir analysis has a high percentage of error rate compared to an automated system. In a company industry, especially in the WWT (Waste Water Treatment) section, it has several reservoir tanks as stock which are still counted manually. The ultrasonic sensor is placed at the top of the WWT tank in a hanging position. Basically, to measure the volume in a tank only variable height is always changing. So by utilizing the function of the ultrasonic sensor and also the tube volume formula, the stored AIR volume can be monitored in real time based on IoT using the Blynk application. From the sensor, height data is obtained which then the formula is processed by Arduino Wemos and then information is sent to the MySQL database server via the WIFI network.


In automated control systems for technical processes, the conversion of a continuous signal into a digital code and vice versa from a digital code to a continuous (analog) value is widely used. For direct type converters often used the term ADC, the reverse - DAC. The characteristics of the converters often dramatically affect the parameters of the entire automated system. The importance of the correct choice of ADCs and DACs has especially increased recently in connection with the mass introduction of microcontrollers MC. Indeed, in addition to the ADC and DAC, it is necessary to place the processor core in the microcontroller's crystal, I/O interfaces and many other elements necessary for the functioning of the MC. The use of information converters in the construction industry imposes additional requirements on converters: for example, in building monitoring systems, precision ADCs with extremely high accuracy are often required (while performance may be low), in other applications it is necessary to provide the necessary parameters at a high level of industrial interference, etc. This article explores issues related to the rational choice of ADCs and DACs, taking into account current trends in the IT field and the specifics of work in the construction industry. Sigma-Delta converters are noted as the most promising models of direct type converters.


Author(s):  
Jacqueline Tran ◽  
Dianne Bautista ◽  
Zoltan Seres ◽  
Loic Cornaut ◽  
Torquil Gundlach ◽  
...  

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