Extended storage of human platelets at 22 C: changes in glycogen and adenine nucleotide metabolism

Transfusion ◽  
1980 ◽  
Vol 20 (2) ◽  
pp. 153-158 ◽  
Author(s):  
A Orengo ◽  
B Lichtiger ◽  
JR Harper
Keyword(s):  
Adenine Nucleotide ◽  
Human Platelets ◽  
Nucleotide Metabolism ◽  
Adenine Nucleotide Metabolism

Adenine Nucleotide Metabolism of Human Platelets during Collagen-Induced Aggregation

1972 ◽  
Vol 27 (03) ◽  
pp. 416-424
Author(s):  
M Murakami ◽  
K Yoshino ◽  
M Takase ◽  
K Odake
Keyword(s):  
Light Transmission ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Rapid Change ◽  
Human Platelets ◽  
Nucleotide Metabolism ◽  
Intracellular Atp ◽  
Adenine Nucleotide Metabolism ◽  
Induced Aggregation

SummaryChanges in platelet adenine nucleotides during collagen-induced aggregation were estimated. Averaged values of ATP and ADP in intact platelets were 56.3 and 28.9 attomoles per platelet, respectively. After collagen-induced aggregation, intracellular ATP plus ADP was depleted to about half of that in intact platelets. The released ADP accounted for 13% of that in intact platelets.Behavior of platelet adenine nucleotides during aggregation was divided into the following phases. At first, platelet ATP decreased without the release of nucleotides. The release of ADP occurred at the stage of rapid change of light transmission. Subsequently, the released ADP decomposed in parallel with the degradation of intracellular ADP. The radioactive ATP in platelets decreased during exposure to collagen, without appreciable release of radioactive nucleotides. The ATP released from disrupted platelets was rapidly metabolized in plasma.

Adenine Nucleotide Metabolism during the Aggregation of Human Platelets by Bovine Factor VIII

1975 ◽  
Vol 4 (1) ◽  
pp. 3-11
Author(s):  
Anne-Carine Østvold ◽  
May Elisabeth Lindberg ◽  
Nils Olav Solum ◽  
Holm Holmsen
Keyword(s):  
Factor Viii ◽  
Adenine Nucleotide ◽  
Human Platelets ◽  
Nucleotide Metabolism ◽  
Adenine Nucleotide Metabolism ◽  
Bovine Factor

Adenine Nucleotide Metabolism of Human Platelets

1971 ◽  
Vol 25 (02) ◽  
pp. 223-233
Author(s):  
M Murakami ◽  
K Odake
Keyword(s):  
Adenine Nucleotide ◽  
Platelet Rich Plasma ◽  
Adenine Nucleotides ◽  
Human Platelets ◽  
Nucleotide Metabolism ◽  
The Other ◽  
Supernatant Fraction ◽  
Two Dimensional ◽  
Adenine Nucleotide Metabolism ◽  
Layer Chromatography

SummaryAfter platelet-rich plasma was incubated with radioactive adenine, radioactive adenine nucleotides in platelets were separated by two-dimensional thin-layer chromatography.Radioactive adenine was selectively incorporated into adenine nucleotides. Gradual decomposition of labelled nucleotides was observed after longer period of incubation. Radioactive ATP, ADP, AMP, IMP, and hypoxanthine were separated from PCA extract of platelets. On the other hand, radioactive adenine and hypoxanthine were separated from platelet-poor plasma.After thrombin treatment, radioactive ATP in platelets broke down rapidly, while radioactive ADP decreased more slowly. Radioactive AMP increased at first in the cellular and supernatant fractions, and then decreased gradually. The accumulation of radioactive hypoxanthine was observed in the supernatant fraction. Released radioactive ATP and ADP were 23% and 22% of the initial radioactive ATP and ADP in platelets, respectively.

The Platelet of the Newborn Infant – Adenine Nucleotide Metabolism and Release

1980 ◽  
Vol 43 (02) ◽  
pp. 099-103 ◽  
Author(s):  
J M Whaun ◽  
P Lievaart ◽  
Keyword(s):  
Newborn Infant ◽  
Adenine Nucleotide ◽  
Platelet Rich Plasma ◽  
Adenine Nucleotides ◽  
Newborn Infants ◽  
Specific Radioactivity ◽  
Nucleotide Metabolism ◽  
Breakdown Product ◽  
Term Infants ◽  
Adenine Nucleotide Metabolism

SummaryBlood from normal full term infants, mothers and normal adults was collected in citrate. Citrated platelet-rich plasma was prelabelled with 3H-adenine and reacted with release inducers, collagen and adrenaline. Adenine nucleotide metabolism, total adenine nucleotide levels and changes in sizes of these pools were determined in platelets from these three groups of subjects.At rest, the platelet of the newborn infant, compared to that of the mother and normal adult, possessed similar amounts of adenosine triphosphate (ATP), 4.6 ± 0.2 (SD), 5.0 ± 1.1, 4.9 ± 0.6 µmoles ATP/1011 platelets respectively, and adenosine diphosphate (ADP), 2.4 ± 0.7, 2.8 ± 0.6, 3.0 ± 0.3 umoles ADP/1011 platelets respectively. However the marked elevation of specific radioactivity of ADP and ATP in these resting platelets indicated the platelet of the neonate has decreased adenine nucleotide stores.In addition to these decreased stores of adenine nucleotides, infant platelets showed significantly impaired release of ADP and ATP on exposure to collagen. The release of ADP in infants, mothers, and other adults was 0.9 ± 0.5 (SD), 1.5 ± 0.5, 1.5 ± 0.1 umoles/1011 platelets respectively; that of ATP was 0.6 ± 0.3, 1.0 ± 0.1,1.3 ± 0.2 µmoles/1011 platelets respectively. With collagen-induced release, platelets of newborn infants compared to those of other subjects showed only slight increased specific radioactivities of adenine nucleotides over basal levels. The content of metabolic hypoxanthine, a breakdown product of adenine nucleotides, increased in both platelets and plasma in all subjects studied.In contrast, with adrenaline as release inducer, the platelets of the newborn infant showed no adenine nucleotide release, no change in total ATP and level of radioactive hypoxanthine, and minimal change in total ADP. The reason for this decreased adrenaline reactivity of infant platelets compared to reactivity of adult platelets is unknown.Infant platelets may have different membranes, with resulting differences in regulation of cellular processes, or alternatively, may be refractory to catecholamines because of elevated levels of circulating catecholamines in the newborn period.

scholarly journals L-Arginine Intake Effect on Adenine Nucleotide Metabolism in Rat Parenchymal and Reproductive Tissues

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
G. Kocic ◽  
J. Nikolic ◽  
T. Jevtovic-Stoimenov ◽  
D. Sokolovic ◽  
H. Kocic ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Tissue Growth ◽  
Nucleotide Metabolism ◽  
Amp Deaminase ◽  
Male Impotence ◽  
Adenine Nucleotide Metabolism ◽  
Adenosine Concentration ◽  
Male Wistar Rats

L-arginine is conditionally essetcial amino acid, required for normal cell growth, protein synthesis, ammonia detoxification, tissue growth and general performance, proposed in the treatment of men sterility and prevention of male impotence. The aim of the present paper was to estimate the activity of the enzymes of adenine nucleotide metabolism:5′-nucleotidase (5′-NU), adenosine deaminase (ADA), AMP deaminase, and xanthine oxidase (XO), during dietary intake of L-arginine for a period of four weeks of male Wistar rats. Adenosine concentration in tissues is maintained by the relative activities of the adenosine-producing enzyme,5′-NU and the adenosine-degrading enzyme-ADA adenosine deaminase. Dietary L-arginine intake directed adenine nucleotide metabolism in liver, kidney, and testis tissue toward the activation of adenosine production, by increased5′-NU activity and decreased ADA activity. Stimulation of adenosine accumulation could be of importance in mediating arginine antiatherosclerotic, vasoactive, immunomodulatory, and antioxidant effects. Assuming that the XO activity reflects the rate of purine catabolism in the cell, while the activity of AMP deaminase is of importance in ATP regeneration, reduced activity of XO, together with the increased AMP-deaminase activity, may suggest that adenine nucleotides are presumably directed to the ATP regenerating process during dietary L-arginine intake.

The Murine Misty Mutation: Phenotypic Effects on Melanocytes, Platelets and Brown Fat

Genetics ◽  
1998 ◽  
Vol 148 (1) ◽  
pp. 381-390
Author(s):  
Elena V Sviderskaya ◽  
Edward K Novak ◽  
Richard T Swank ◽  
Dorothy C Bennett
Keyword(s):  
Coat Color ◽  
Adenine Nucleotide ◽  
Primary Cultures ◽  
Cell Types ◽  
Nucleotide Metabolism ◽  
Brown Fat ◽  
Melanocyte Stimulating Hormone ◽  
Prolonged Bleeding Time ◽  
Adenine Nucleotide Metabolism

Abstract Although the recessive murine mutation misty (m) is well known, its phenotype has never been reported beyond brief descriptions of a dilution of coat color and white spotting of the belly and extremities, suggesting a developmental mutation. A report in abstract has also suggested effects on white fat and body weight. Here, we report effects of the homozygous misty mutation on an unusual combination of three cell types: melanocytes, platelets, and brown fat. Brown fat appeared to be completely absent from all expected locations in neonatal m/m mice. A prolonged bleeding time was observed; platelet count and platelet serotonin and ATP levels were normal, but the level of ADP in m/m platelets was low. Primary cultures and immortal lines of melanocytes from m/m mice showed several abnormalities. There was a marked deficiency in net proliferation, suggesting that the color dilution and spotting in vivo may result from reduced numbers of melanocytes and their precursors. m/m melanocytes were also hyperdendritic in morphology, overproduced melanin, and had deficient responses to the cAMP agonists cholera toxin and melanocyte-stimulating hormone, which normally promote melanin production. The misty gene product may be involved in adenine nucleotide metabolism or signaling.

Adenine Nucleotide Metabolism in Liver Ischemia: Effect of Allopurinol

1991 ◽  
pp. 301-304 ◽  
Author(s):  
Felícitas A. Mateos ◽  
Ma. Victoria Díaz ◽  
Juan G. Puig ◽  
Teresa H. Ramos ◽  
Manuel L. Jiménez ◽  
...  
Keyword(s):  
Adenine Nucleotide ◽  
Nucleotide Metabolism ◽  
Liver Ischemia ◽  
Adenine Nucleotide Metabolism

Effects of reserpine and serotonin on adenine nucleotide metabolism and glucose oxidation of washed rabbit platelets

1977 ◽  
Vol 26 (18) ◽  
pp. 1667-1670 ◽  
Author(s):  
Hans-Joachim Reimers ◽  
Raelene L. Kinlough-Rathbone ◽  
Marian A. Packham ◽  
J.Fraser Mustard
Keyword(s):  
Glucose Oxidation ◽  
Adenine Nucleotide ◽  
Nucleotide Metabolism ◽  
Adenine Nucleotide Metabolism ◽  
Rabbit Platelets
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