In this paper, the mathematical model based on modern design methods of machinery used to optimize the parameters of the two-stage chain transmission system was established. The optimization toolbox of Matlab was introduced as well. Optimization results showed that, the pitches of first and second-stage chains were 19.05 and 25.4 respectively; compared with the original scheme, transmission ratio of first-stage chain decrease from 2.57 to 1.77, while the second-stage increase from 1.0 to 1.45. The number of chain strands of second-stage chain decreased from 2 to 1. The teeth number of the driven sprocket of the first-stage chain decreased also from 59 to 41. Total weight of the sprockets of the chain transmission system decreased from 25.40 kg to 14.84 kg, at least 41.57% material was saved and not less than 50% production cost was reduced. The optimization method is simple, feasible and the optimized parameters are reliable in practice.