As for some large-scale and arid irrigation district in China, such as Jinghuiqu irrigation district and Shihezi reclamation district, hydro-ecosystem was gradually degenerated and imbalanced due to water shortage and unreasonable exploitation. In order to study hydrological elements, water cycle process and ecological response caused by human activities and other factors, and improve the quality of hydro-ecosystem, scientific connotation of establishing hydro-eco dynamic monitoring system in large-scale and arid irrigation district was defined. Hydro-ecosystem dynamic monitoring indexes including meteorological elements, hydrological elements, ecological elements closely related to hydrological elements and cycle, and socio-economic elements were proposed. Comprehensive monitoring methods based on automatic monitoring net, remote sensing satellite monitoring, field test and investigation were determined, and overall framework of hydro-eco dynamic monitoring was designed, which provided a theoretical basis for establishing hydro-eco dynamic monitoring system in large-scale and arid irrigation district.