Trout (212 brook trout, Salvelinus fontinalis, and 231 brown trout, Salmo trutta, Salmonidae) were collected from the Au Sable River, Michigan, and examined for parasites between April 1982 and July 1984. One hundred seventy-six brook trout and 153 brown trout were infected with at least one of the following parasites: Crepidostomum cooperi, Neascus sp., Eubothrium sp., Proteocephalus sp., Truttaedacnitis sp., Cystidicoloides tenuissima, Rhabdochona canadensis, Spinitectus gracilis, Epistylis sp., Trichodina sp., and Salmincola edwardsii. Cystidicoloides tenuissima, the most common and abundant species, did not exhibit a pronounced seasonal pattern in prevalence; mean intensity, however, was highest in July 1982, 1983, and 1984 in both trout species. The intensity of C. tenuissima increased as trout became older and then decreased in brook and brown trout 3 and 4 years of age, respectively. Data on the seasonality of S. gracilis and C. cooperi infecting trout are also presented. The muscles of trout were negative for parasites. Over 500 mayfly nymphs (Ephemeroptera) representing at least seven species were examined for parasites. Cystidicoloides tenuissima infected Ephemera simulans. Crepidostomum sp., Rhabdochona sp., and S. gracilis occurred in Hexagenia limbata.