Immunocytochemical and ultrastructural characterization of endocrine cells in the larval stomach of the frog Rana temporaria tadpoles: a comparison with adult specimens

Tracheal endocrine cells (TECs) that contain serotonin have been characterized previously by staining with ferric ferricyanide. In the present article, the ferric ferricyanide staining reaction has been used to locate the TECs in deplasticized thick sections of Epon-embedded rabbit tracheas. Adjacent thin sections of the same cell were subsequently observed by electron microscopy. The TECs were filled with dense-core vesicles (DCVs) located in the cytoplasm between the nucleus and the lumen and also lateral to the nucleus. In a separate experiment, pieces of rabbit trachea were treated with a solution of glutaraldehyde-dichromate to demonstrate the presence of amines. High levels of chromium were detected in the DCVs by energy-dispersive X-ray analysis. The results from these studies have correlated the ultrastructure of a serotonin-containing endocrine cell present in rabbit tracheal epithelium with a cell type previously characterized only by light and fluorescence histochemical methods. The results also indicate that serotonin in these cells is stored in the DCVs.

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Immunocytochemical and ultrastructural characterization of coexistence of pancreatic polypeptide and glucagon-like immunoreactivity in the pancreatic endocrine cells of Sparus auratus L. (Teleostei)

General and Comparative Endocrinology ◽

10.1016/0016-6480(88)90089-5 ◽

1988 ◽

Vol 70 (1) ◽

pp. 9-19 ◽

Cited By ~ 21

Author(s):

M.E. Abad ◽

J.J. Taverne-Thiele ◽

J.H.W.M. Rombout

Keyword(s):

Pancreatic Polypeptide ◽

Endocrine Cells ◽

Ultrastructural Characterization ◽

Pancreatic Endocrine Cells ◽

Sparus Auratus

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Phenetic peculiarities of biochorological groups of different scales in a common frog (Rana temporaria linnaeus)

Herald of Tver State University. Series: Biology and Ecology ◽

10.26456/vtbio209 ◽

2021 ◽

pp. 19-38

Author(s):

Алла Александровна Емельянова ◽

Наталья Евгеньевна Николаева ◽

Елена Андреевна Гурская

Keyword(s):

Genetic Structure ◽

Rana Temporaria ◽

Spatial Genetic Structure ◽

Dorsal Surface ◽

The Body ◽

Common Frog ◽

Detailed Characterization ◽

Frog Rana Temporaria ◽

Spatial Isolation

На примере серий лягушки травяной из четырех биотопов, характеризующихся разной степенью пространственной изоляции, показана перспективность изучения биохорологической структуры данного вида с применением фенетического подхода. Наиболее детальную характеристику пространственно-генетической структуры можно получить при анализе пятнистости дорзальной поверхности тела. Так же для этих целей возможно использование вариаций таких элементов рисунка покровов тела, как форма межлопаточного пятна и число полос на бедре. The prospects of studying the biochorological structure of the selected species using a phenetic approach are shown in a series of common frogs from four biotopes characterized by varying degrees of spatial isolation. The most detailed characterization of the spatial-genetic structure can be obtained by analyzing the spotting of the dorsal surface of the body. Also, for these purposes, it is possible to use variations of such elements of the body covering pattern as the shape of the interscapular spot and the number of stripes on the thigh.

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