A Rapid, Sensitive and Validated Method for the Determination of Ondansetron in Human Plasma by Reversed-phase High-pressure Liquid Chromatography

2011 ◽  
Vol 54 (10) ◽  
pp. 655-659 ◽  
Author(s):  
Durairaj Chandrasekar ◽  
Sistla Ramakrishna ◽  
Prakash Diwan
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Pressure Liquid Chromatography ◽  
Reversed Phase

Determination of deuterium-labeled phenylalanine and tyrosine in human plasma with high pressure liquid chromatography and mass spectrometry

1976 ◽  
Vol 73 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Friedrich K. Trefz ◽  
Dennis J. Byrd ◽  
Milan E. Blaskovics ◽  
Walter Kochen ◽  
Peter Lutz
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Pressure Liquid Chromatography

scholarly journals Quantitative profiling of 1-Carbon Metabolites, Amino Acids and Precursors, and Plasmalogens in human plasma using Ultra-High-Pressure Liquid Chromatography coupled with Tandem Mass Spectrometry and robotic compound extraction

2019 ◽  
Author(s):  
Stephanie Andraos ◽  
Michael Goy ◽  
Ben Albert ◽  
Martin Kussmann ◽  
Eric B. Thorstensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
High Pressure Liquid Chromatography ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Ultra High Pressure

AbstractAmino acids (AAs) and one-carbon (1-C) compounds are involved in a range of key metabolic pathways, and mediate numerous health and disease processes in the human body. Previous assays have quantified a limited selection of these compounds. Here, we describe an analytical method for the simultaneous quantification of 37 1-C metabolites, amino acids, precursors, and plasmalogens using reversed-phase ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Compound extraction from human plasma was tested manually before being robotically automated. The final analytical panel was validated on human plasma samples. Our automated and multiplexed method holds promise for application to large cohort studies.

Determination of acetaminophen concentrations in serum by high-pressure liquid chromatography.

1977 ◽  
Vol 23 (9) ◽  
pp. 1596-1598 ◽  
Author(s):  
R A Horvitz ◽  
P I Jatlow
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Diethyl Ether ◽  
High Pressure Liquid Chromatography ◽  
Internal Standard ◽  
Reversed Phase ◽  
Single Extraction ◽  
Toxic Concentrations

Abstract We describe a method for determination of serum acetaminophen concentrations in serum by reversed phase high-pressure liquid chromatography. The homolog N-propionyl-p-aminophenol was used as an internal standard. The procedure, which requires only a single extraction with diethyl ether, can be optimized to be linear over the ranges of 10 to 100 or 1 to 20 mg/liter. Within-run CV was 1.2%; between-run CV was 4.4% and 4.9% at two different concentrations. Many commonly used drugs were tested and found not to interfere. The procedure is simple and rapid enough for use on an emergency basis in cases of overdosage, and can be optimized for measurement of either therapeutic or toxic concentrations.

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