A Simple HPLC Method with Fluorescence Detection for Simultaneous Determination of 10-methoxycamptothecin and its Metabolite 10-hydroxycamptothecin in Rat Liver Tissue

Drug Research ◽  
2014 ◽  
Vol 65 (03) ◽  
pp. 147-152 ◽  
Author(s):  
Y. Liu ◽  
C. Shao ◽  
B. Fan ◽  
S. Li ◽  
Y. Wang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Liver ◽  
Fluorescence Detection ◽  
Liver Tissue ◽  
Hplc Method

scholarly journals Simultaneous Determination of Amlodipine Besylate and Atorvastatin Calcium in Binary Mixture by Spectrofluorimetry and HPLC Coupled with Fluorescence Detection

2013 ◽  
Vol 8 ◽  
pp. ACI.S12921 ◽  
Author(s):  
Bahia A. Moussa ◽  
Asmaa A. El-Zaher ◽  
Marianne A. Mahrouse ◽  
Maha S. Ahmed
Keyword(s):  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Sensitivity ◽  
Hplc Method ◽  
Detection Methods ◽  
Amlodipine Besylate ◽  
Atorvastatin Calcium ◽  
New Methods ◽  
Spectrofluorimetric Method

Caduet tablets are novel prescription drug that combines amlodipine besylate (AM) with atorvastatin calcium (AT). A spectrofluorimetric and an HPLC-fluorescence detection methods were developed for simultaneous determination of both drugs in tablets. In the spectrofluorimetric method, native fluorescence of AM and AT were measured in methanol at 442 and 369 nm upon excitation at 361 and 274 nm, respectively. The emission spectrum of each drug reveals zero value at the emission wavelength of the other drug, thus allowing their simultaneous determination without interference. In the HPLC method, separation of AM and AT was achieved within 8 minutes on a C18 column using acetonitrile:phosphate buffer (0.015 M, pH 3) (45:55, v/v) as the mobile phase. Fluorescence detection was carried out using excitation wavelengths 361 and 274 nm and emission wavelengths 442 and 378 nm for AM and AT, respectively. Excellent linearity was observed. Careful validation proved advantages of the new methods: high sensitivity, accuracy, selectivity and suitability for quality control laboratories.

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