Click-Chemistry Based Enrichment Strategy Extends the Detectable Metabolome in LC-HRMS/MS Analysis

Synfacts ◽  
2020 ◽  
Vol 17 (01) ◽  
pp. 0102
Keyword(s):  
Click Chemistry ◽  
Ms Analysis ◽  
Enrichment Strategy

Strategy for the enrichment of protein biomarkers from diverse bacterial select agents

2021 ◽  
Vol 28 ◽  
Author(s):  
Sasikumar Sabna ◽  
Dev Vrat Kamboj ◽  
Ravi Bhushan Kumar ◽  
Prabhakar Babele ◽  
Sakshi Rajoria ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Immune Serum ◽  
Tandem Ms ◽  
Environmental Matrices ◽  
Bacterial Proteins ◽  
Conserved Regions ◽  
Groel Protein ◽  
Ms Analysis ◽  
Enrichment Strategy

Background: Some pathogenic bacteria can be potentially used for nefarious applications in the event of bioterrorism or biowarfare. Accurate identification of biological agent from clinical and diverse environmental matrices is of paramount importance for implementation of medical countermeasures and biothreat mitigation. Objective: A novel methodology is reported here for the development of a novel enrichment strategy for the generally conserved abundant bacterial proteins for an accurate downstream species identification using tandem MS analysis in biothreat scenario. Methods: Conserved regions in the common bacterial protein markers were analyzed using bioinformatic tools and stitched for a possible generic immuno-capture for an intended downstream MS/MS analysis. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of 60 kDa chaperonin GroEL. Hyper-immune serum was raised against recombinant synthetic GroEL protein. Results: The conserved regions of common bacterial proteins were stitched for a possible generic immuno-capture and subsequent specific identification by tandem MS using variable regions of the molecule. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of GroEL. In a proof-of-concept study, hyper-immune serum raised against recombinant synthetic GroEL protein exhibited reactivity with ~60 KDa proteins from the cell lysates of three bacterial species tested. Conclusion: The envisaged methodology can lead to the development of a novel enrichment strategy for the abundant bacterial proteins from complex environmental matrices for the downstream species identification with increased sensitivity and substantially reduce the time-to-result.

Method for full protein sequence mapping: LC-MS analysis

2007 ◽  
Author(s):  
Erin D. Jeffery
Keyword(s):  
Protein Sequence ◽  
Sequence Mapping ◽  
Ms Analysis

HPLC-MS analysis of resveratrol in different nutritions

2011 ◽  
Vol 49 (05) ◽  
Author(s):  
S Szabó ◽  
L Márk ◽  
S Kiss ◽  
É Polyák ◽  
A Kisbenedek ◽  
...  
Keyword(s):  
Ms Analysis

LC-ESI-MS/MS analysis of Quassia amara leaves tea. Is antiplasmodial activity of the tea is due to quassinoids?

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
N Fabre ◽  
E Deharo ◽  
HL Le ◽  
C Girardi ◽  
A Valentin ◽  
...  
Keyword(s):  
Antiplasmodial Activity ◽  
Esi Ms ◽  
Ms Analysis ◽  
Quassia Amara

MS Analysis of Retinoids by Atmospheric Solids Atomization Probe

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
S Dobreniecki ◽  
JR Porter
Keyword(s):  
Ms Analysis

Qualitative and quantitative LC-MS analysis of different Rhodiola rosea rhizome extracts

2019 ◽  
Author(s):  
F Alperth ◽  
I Turek ◽  
S Weiß ◽  
D Vogt ◽  
F Bucar
Keyword(s):  
Rhodiola Rosea ◽  
Qualitative And Quantitative ◽  
Ms Analysis

Click Chemistry Expedited Radiosynthesis: Sulfur [18F]fluoride Exchange of Aryl Fluorosulfates

2019 ◽  
Author(s):  
Qinheng Zheng ◽  
Hongtao Xu ◽  
Hua Wang ◽  
Wen-Ge Han Du ◽  
Nan Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
High Performance ◽  
Isotopic Exchange ◽  
Tumor Imaging ◽  
Radiochemical Yield ◽  
Exchange Method ◽  
Molar Activity ◽  
Positron Emission ◽  
Sulfur Fluoride

The lack of simple, efficient [<sup>18</sup>F]fluorination processes and new target-specific organofluorine probes remains the major challenge of fluorine-18-based positron emission tomography (PET). We report here a fast isotopic exchange method for the radiosynthesis of aryl [<sup>18</sup>F]fluorosulfate based PET agents enabled by the emerging sulfur fluoride exchange (SuFEx) click chemistry. The method has been applied to the fully-automated <sup>18</sup>F-radiolabeling of twenty-five structurally diverse aryl fluorosulfates with excellent radiochemical yield (83–100%) and high molar activity (up to 281 GBq µmol<sup>–1</sup>) at room temperature in 30 seconds. The purification of radiotracers requires no time-consuming high-performance liquid chromatography (HPLC), but rather a simple cartridge filtration. The utility of aryl [<sup>18</sup>F]fluorosulfate is demonstrated by the <i>in vivo</i> tumor imaging by targeting poly(ADP-ribose) polymerase 1 (PARP1).

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR &amp; GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

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