Leukocyte Alkaline Phosphatase Activity in Rickets Due to Vitamin D Deficiency

1969 ◽  
Vol 280 (22) ◽  
pp. 1221-1223 ◽  
Author(s):  
Şinasi Özsoylu
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Vitamin D Deficiency ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Leukocyte Alkaline Phosphatase

scholarly journals Identification of intestinal cells responsive to calcitriol (1,25-dihydroxycholecalciferol)

1985 ◽  
Vol 225 (1) ◽  
pp. 127-133 ◽  
Author(s):  
M W Smith ◽  
M E Bruns ◽  
E D M Lawson
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Quantitative Description ◽  
Intestinal Cells ◽  
Protein Levels ◽  
High Affinity ◽  
Hormone Injection ◽  
Crypt Cells

The location of intestinal cells taken from the base of the crypt to the tip of the villus responsive to calcitriol (1,25-dihydroxycholecalciferol) and the distribution of [3H]calcitriol within the intestinal epithelium has been determined in vitamin D-deficient rats. The calcitriol responses examined were CaBP (Ca2+-binding protein) levels as measured by immunodiffusion and alkaline phosphatase levels as determined cytochemically. Calcitriol had no effect on villus structure or on enterocyte kinetics. This made it possible to compare levels of CaBP and alkaline phosphatase activity in enterocytes at different ages in rats at known times after hormone injection. Cells from both the crypt and villus synthesized CaBP in response to calcitriol. Alkaline phosphatase activity was not detectable in crypt cells, although a pool of precursor was produced in these cells in response to calcitriol. Enzyme activity was increased in all villus cells in response to calcitriol, but the quantitative description of this effect was very different from that found for calcitriol effects on CaBP synthesis. Calcitriol injected into vitamin D-deficient rats was detected, within 2h, in all cells of the crypt and villus. Most of the binding was to sites having a high affinity for the injected hormone.

scholarly journals Endocrine Relationships of Leukocyte Alkaline Phosphatase

Blood ◽  
1965 ◽  
Vol 25 (3) ◽  
pp. 356-369 ◽  
Author(s):  
FRED ROSNER ◽  
STANLEY L. LEE
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Mean Value ◽  
Conclusive Evidence ◽  
In Vitro Tests ◽  
Enzyme Release ◽  
Men And Women ◽  
Leukocyte Alkaline Phosphatase

Abstract Leukocyte alkaline phosphatase activity has been noted to be different in men and women. The mean leukocyte alkaline phosphatase activity for 74 normal men, aged 19 to 60 years, was 23 mg. of phosphorus per 1010 polvmorphonuclear leukocytes per hour. The corresponding mean value for 75 normal young women, age 19-48 years, was 35 (p < .001). No significant differences between boys and girls occurred until the time of puberty. After the menopause, the values for women approached the values for men. Women treated with androgens had lower leukocyte alkaline phosphatase activity than did control women. These results suggest that androgenic hormones inhibit this enzyme, and that other, as yet undefined endocrine influences, also affect its level of activity. In vitro tests with various concentrations of androgens and estrogens failed to provide conclusive evidence of direct effect on leukocytes although some degree of direct inhibition by androgens was suggested. Studies using saponin to effect enzyme release from leukocyte granules did not demonstrate whether the differences between men and women are differences of enzyme release or of content of leukocyte alkaline phosphatase.

Enhancement of vitamin D3 effect on bone metabolism in weanling rats orally administered zinc sulphate

1986 ◽  
Vol 111 (2) ◽  
pp. 285-288 ◽  
Author(s):  
Masayoshi Yamaguchi ◽  
Teruyuki Sakashita
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Bone Metabolism ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Dna Content ◽  
Zinc Sulphate ◽  
Simultaneous Administration ◽  
Weanling Rats ◽  
Stimulation Of

Abstract. The interaction of vitamin D3 and zinc on bone metabolism was investigated in the femur of weanling rats. Oral administration of vitamin D3 (1.0 μg/100 g body weight) did not cause any increase in the zinc accumulation in the femoral tissue following treatment with zinc sulphate (1.0 mg Zn/100 g). Administration of vitamin D3 or zinc produced significant increases the alkaline phosphatase activity and DNA content of the femoral diaphvsis but not of the epiphysis. The increase in alkaline phosphatase activity was enhanced additionally by simultaneous administration of vitamin D3 and zinc. Moreover, the increase in DNA content was enhanced markedly (about 4 times) by these treatments. At a dose of 0.5 μg of vitamin D3 per 100 g, DNA content was at the control level. This level was increased about 2 times by simultaneous administration of zinc (1.0 mg/100 g). The increase in alkaline phosphatase activity following simultaneous administration of vitamin D3 and zinc was significantly inhibited by treatment with cycloheximide, actinomycin D, or mitomycin C. Also, the increase in DNA content was completely inhibited by mitomycin C treatment. The present data suggest that the combination of vitamin D3 and zinc has a multiple effect on the stimulation of bone growth and mineralization in weanling rats, and that this effect is based on a stimulation of the DNA synthesis in bone cells.

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