Alkaline-Phosphatase Activity in Developing Molars of Vitamin D-deficient Rats. I. High Calcium-Phosphorus Ratio Diet

1964 ◽  
Vol 43 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Enrique Kiguel
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
High Calcium ◽  
Calcium Phosphorus

scholarly journals Identification of intestinal cells responsive to calcitriol (1,25-dihydroxycholecalciferol)

1985 ◽  
Vol 225 (1) ◽  
pp. 127-133 ◽  
Author(s):  
M W Smith ◽  
M E Bruns ◽  
E D M Lawson
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Quantitative Description ◽  
Intestinal Cells ◽  
Protein Levels ◽  
High Affinity ◽  
Hormone Injection ◽  
Crypt Cells

The location of intestinal cells taken from the base of the crypt to the tip of the villus responsive to calcitriol (1,25-dihydroxycholecalciferol) and the distribution of [3H]calcitriol within the intestinal epithelium has been determined in vitamin D-deficient rats. The calcitriol responses examined were CaBP (Ca2+-binding protein) levels as measured by immunodiffusion and alkaline phosphatase levels as determined cytochemically. Calcitriol had no effect on villus structure or on enterocyte kinetics. This made it possible to compare levels of CaBP and alkaline phosphatase activity in enterocytes at different ages in rats at known times after hormone injection. Cells from both the crypt and villus synthesized CaBP in response to calcitriol. Alkaline phosphatase activity was not detectable in crypt cells, although a pool of precursor was produced in these cells in response to calcitriol. Enzyme activity was increased in all villus cells in response to calcitriol, but the quantitative description of this effect was very different from that found for calcitriol effects on CaBP synthesis. Calcitriol injected into vitamin D-deficient rats was detected, within 2h, in all cells of the crypt and villus. Most of the binding was to sites having a high affinity for the injected hormone.

Enhancement of vitamin D3 effect on bone metabolism in weanling rats orally administered zinc sulphate

1986 ◽  
Vol 111 (2) ◽  
pp. 285-288 ◽  
Author(s):  
Masayoshi Yamaguchi ◽  
Teruyuki Sakashita
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Bone Metabolism ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Dna Content ◽  
Zinc Sulphate ◽  
Simultaneous Administration ◽  
Weanling Rats ◽  
Stimulation Of

Abstract. The interaction of vitamin D3 and zinc on bone metabolism was investigated in the femur of weanling rats. Oral administration of vitamin D3 (1.0 μg/100 g body weight) did not cause any increase in the zinc accumulation in the femoral tissue following treatment with zinc sulphate (1.0 mg Zn/100 g). Administration of vitamin D3 or zinc produced significant increases the alkaline phosphatase activity and DNA content of the femoral diaphvsis but not of the epiphysis. The increase in alkaline phosphatase activity was enhanced additionally by simultaneous administration of vitamin D3 and zinc. Moreover, the increase in DNA content was enhanced markedly (about 4 times) by these treatments. At a dose of 0.5 μg of vitamin D3 per 100 g, DNA content was at the control level. This level was increased about 2 times by simultaneous administration of zinc (1.0 mg/100 g). The increase in alkaline phosphatase activity following simultaneous administration of vitamin D3 and zinc was significantly inhibited by treatment with cycloheximide, actinomycin D, or mitomycin C. Also, the increase in DNA content was completely inhibited by mitomycin C treatment. The present data suggest that the combination of vitamin D3 and zinc has a multiple effect on the stimulation of bone growth and mineralization in weanling rats, and that this effect is based on a stimulation of the DNA synthesis in bone cells.

EFFECT OF NORETHANDROLONE ON SELECTED CONNECTIVE TISSUES

1965 ◽  
Vol 43 (8) ◽  
pp. 1235-1241 ◽  
Author(s):  
J. D. Cipera ◽  
B. B. Migicovsky ◽  
L. F. Belanger
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Water Content ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Epiphyseal Cartilage ◽  
Prolonged Treatment ◽  
Radioactive Tracers ◽  
Hydroxyproline Content ◽  
Connective Tissues

The effect of norethandrolone (17α-ethyl-19-nortestosterone) on the epiphyseal cartilage of rachitic chicks was investigated in conjunction with the studies of vitamin D3 effect on this tissue. Norethandrolone effected a significant decrease in the activity of hexosamine synthetase, but did not alter appreciably the levels of hexosamine or hydroxyproline, nor the activity of alkaline phosphatase.It was noted that norethandrolone exerted a powerful stimulation on the growth of combs of immature chicks. The increased weight of the combs was accompanied by a higher water content. The de-fatted and dried fraction of these combs had an elevated content of hexosamines, while the decrease in hydroxyproline content was noted only after a prolonged treatment with the steroid. Studies with radioactive tracers (Na2S35O4, NaOAc-1-C14) showed an increased rate of incorporation of these potential components of glycosaminoglycans in the de-fatted and dried fraction of the combs. In addition, norethandrolone enhanced the alkaline phosphatase activity in combs.

Transient hyperphosphatasemia in a child with autosomal recessive vitamin D dependency.

1986 ◽  
Vol 32 (7) ◽  
pp. 1418-1419 ◽  
Author(s):  
D E Cole
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Disease ◽  
Autosomal Recessive ◽  
Metabolic Bone Disease ◽  
Extensive Investigation ◽  
Asymptomatic Children ◽  
Metabolic Bone

Abstract The case of a 4.5-year-old girl with autosomal recessive vitamin D dependency is described. Although she had been effectively treated since one month postpartum with 1 alpha-hydroxycholecalciferol [1 alpha(OH)D3, alfacalcidol], her mean alkaline phosphatase (EC 3.1.3.1) activity in serum increased to 3680 U/L from a stable value [335 (SD 50) U/L; n = 12] within three weeks, then returned to baseline over the ensuing four months. Transient hyperphosphatasemia was diagnosed. Extensive investigation of an isolated episodic increase in alkaline phosphatase activity is as superfluous in the child with adequately treated metabolic bone disease as it is in other healthy and asymptomatic children.

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