The incidence of barley yellow dwarf viruses in wheat in Victoria

1987 ◽  
Vol 38 (5) ◽  
pp. 821 ◽  
Author(s):  
RJ Sward ◽  
RM Lister
Keyword(s):  
Immunosorbent Assay ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barley Yellow Dwarf ◽  
Specific Antisera ◽  
Yellow Dwarf Virus ◽  
One Year ◽  
Pooled Samples

Wheat crops from all major wheat-growing districts throughout Victoria were sampled during September 1984. Examination of pooled samples by enzyme-linked immunosorbent assay (ELISA) with an antiserum to a mixture of two barley yellow dwarf virus (BYDV) types ('V1') showed that 10 out of 26 crops were infected with BYDV and 3 out of 26 had a BYDV incidence greater than 10%. The overall loss in yield likely to result from BYDV was estimated at 2% with a far greater loss in some crops. Frozen-stored samples from four crops with high levels of BYDV were retested after one year with isolate-specific antisera. The results indicated that in these crops, PAV-related isolates were the most common, followed by RPV-related isolates.

scholarly journals Expression of Thinopyrum intermedium-Derived Barley yellow dwarf virus Resistance in Elite Bread Wheat Backgrounds

2001 ◽  
Vol 91 (1) ◽  
pp. 55-62 ◽  
Author(s):  
L. Ayala ◽  
M. van Ginkel ◽  
M. Khairallah ◽  
B. Keller ◽  
M. Henry
Keyword(s):  
Bread Wheat ◽  
Seed Quality ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thinopyrum Intermedium ◽  
Mendelian Segregation ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Resistance to Barley yellow dwarf virus (BYDV) is not found in wheat but is available in a Thinopyrum intermedium translocation (Ti) carried on chromosome 7DL of bread wheat recombinant lines. We used one of those lines (TC14/2*Spear) to introgress the Ti into bread wheat cultivars and to determine the influence of wheat backgrounds, with and without known tolerance to BYDV, on the expression of resistance. Two single and three backcross populations, segregating for the presence of the alien fragment, were tested under field conditions and artificial inoculation with BYDV isolates MAV-Mex and PAV-Mex. Lines containing the fragment were identified using the microsatellite marker gwm37. Tillering, biomass, grain yield, thousand-kernel weight, and seed quality were evaluated in inoculated and noninoculated plots. Resistance was assessed by enzyme-linked immunosorbent assay. In early generations, the alien fragment followed expected Mendelian segregation, whereas in the advanced ones a slight bias against its transmission was observed. No positive nor negative effects of Ti on agronomic performance and quality were found. A significant optical density reduction in individuals carrying the fragment was observed after PAV infection in crosses with lines Anza and Baviacora but not with Milan. In addition, the fragment was associated with a lower frequency of infected plants for both PAV and MAV isolates. The reduced yield loss associated with the presence of the translocation was due largely to the lower infection rate.

scholarly journals Comparison of Techniques for Detection of Barley Yellow Dwarf Virus-PAV-IL

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1236-1240 ◽  
Author(s):  
Antonia R. Figueira ◽  
Leslie L. Domier ◽  
Cleora J. D'Arcy
Keyword(s):  
Nucleic Acid ◽  
Barley Yellow Dwarf Virus ◽  
Infected Tissue ◽  
Yellow Dwarf ◽  
Nucleic Acid Hybridization ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hybridization Technique ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Detection of barley yellow dwarf virus (BYDV)-PAV-IL by an improved nucleic acid hybridization technique, using a nonradioactive probe with chromogenic and chemiluminescent substrates, was compared with detection by polymerase chain reaction (PCR), double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antibodies, and triple antibody sandwich ELISA with polyclonal and monoclonal antibodies. Each method was used to detect purified virus and virus in sap extracts from infected oat leaves. The detection limits for both ELISA procedures were 1 ng of purified BYDV-PAV-IL and the equivalent of 78 ng of infected tissue. Nucleic acid hybridization with either chemiluminescent or chromogenic substrates also detected as little as 1 ng of purified BYDV-PAV-IL, but it was slightly more sensitive at detecting virus in tissue extracts (25 ng of infected tissue). The most sensitive detection technique was PCR amplification, which could detect as little as 0.1 pg of RNA extracted from purified virus and detected viral RNA in the equivalent of 0.5 pg of infected leaf tissue.

Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 406-413 ◽  
Author(s):  
H. Sharma ◽  
H. Ohm ◽  
L. Goulart ◽  
R. Lister ◽  
R. Appels ◽  
...  
Keyword(s):  
Chromosome Pairing ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Substitution Lines ◽  
Barley Yellow Dwarf ◽  
Backcross Populations ◽  
Yellow Dwarf Virus ◽  
Wheat Lines

Wheatgrasses (species of Agropyron complex) have previously been reported to be resistant to barley yellow dwarf virus (BYDV). To introgress this resistance into wheat, Triticum aestivum × Thinopyrum (Agropyron) intermedium hybrids were advanced through a backcrossing program and reaction to BYDV, as determined by enzyme-linked immunosorbent assay (ELISA), is reported for the first time in backcross populations of wide hybrids between wheat and wheatgrasses. ELISA values revealed highly resistant to highly susceptible segregants in backcrosses. BYDV resistance was expressed in some backcross derivatives. Continued selection, based on cytology and ELISA in each generation, eliminated most of the unwanted wheatgrass chromosomes and produced self-fertile BYDV resistant wheat lines. The BYDV resistant lines with 2n = 42 had normal chromosome pairing similar to wheat, and their F1 hybrids with wheat had two univalents. DNA analyses showed that the source of alien chromatin in these BYDV resistant wheat lines is distinguishable from that in other Th. intermedium derived BYDV resistant wheat lines. Chromosome pairing and restriction fragment length polymorphism analyses indicated that the 42 chromosome resistant Purdue wheat lines are substitution lines in which chromosome 7D was replaced by a chromosome from Th. intermedium that was carrying gene(s) for BYDV resistance.Key words: BYDV, Agropyron, Thinopyrum, wheat, backcrosses, Southern blots.

Tolerance to Barley Yellow Dwarf Virus in Triticale

Crop Science ◽  
1990 ◽  
Vol 30 (5) ◽  
pp. 1008 ◽  
Author(s):  
J. Collin ◽  
A. Comeau ◽  
C.A. St-Pierre
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus
Sign in / Sign up

Export Citation Format

Share Document