scholarly journals ASM Affairs, November 2005

2005 ◽  
Vol 26 (4) ◽  
pp. 184

Conference report ASM 2005: 25-29 September, National Convention Centre, Canberra; Conference report ASM 2005; Becton-Dickinson Student Awards 2005

2021 ◽  
Vol 15 (3) ◽  
Author(s):  
Anupam Mukherjee ◽  
Divya Taneja ◽  
Anil Khurana

Author(s):  
Etienne de Harven ◽  
Davide Soligo ◽  
Roy McGroarty ◽  
Hilary Christensen ◽  
Richard Leung ◽  
...  

Taking advantage of the high elemental contrast of particles of colloidal gold observed in the backscattered electron imaging(BEI) mode of the SEM (1,2), the human T lymphocyte was chosen as a model system to study the potential value of immunogold labeling for the quantification of cell surface expressed molecules. The CD3 antigen which is expressed on all human T lymphocytes and is readily identified by the LEU-4 murine monoclonal antibody (Becton Dickinson, Mountain View, CA) followed by a gold conjugated goat anti-mouse Ig polyclonal antibody was chosen as a model target antigen. When quantified by non-EM methods, using radio-iodinated probes or FACS analysis, approximately 30,000 to 50,000 copies of this antigen per cell are enumerated.The following observations were made while attempting to quantify the same molecule by SEM after specific immunogold labeling:Imaging in the SE vs BE mode: The numbers of gold markers counted in the secondary electron (SE) imaging mode are considerably lower than those counted on the same cells in the backscattered electron (BE) imaging mode.


2019 ◽  
Vol 16 (2) ◽  
pp. 341-344
Author(s):  
Rana Aytug ◽  
Lee Daly

On December 6, 2018, academics from across Europe and beyond gathered at London’s Freud Museum for a conference on the politics, policies and diplomacy of diaspora governance. This conference was organized by Senior Research Fellow Dr Bahar Baser from the Centre for Trust, Peace and Social Relations (CTPSR), Coventry University, UK and Dr Henio Hoyo, Research Professor from the Department of Social Sciences/School of Law and Social Sciences, Universidad de Monterrey (UDEM) Monterrey, Mexico.


1991 ◽  
Vol 9 (1) ◽  
pp. 59-61
Keyword(s):  

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