Nitrate Reduction by a Dicarboxylate Shuttle in a Reconstituted System from Spinach Leaves
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A reconstituted system of supernatant and mitochondria from spinach leaves reduced NO3- in the presence of NAD+. A faster rate (2-6 times) of NO3- reduction was obtained when oxaloacetate (OAA) and glycine were present. The reduction of NO3- in the presence of OAA and glycine was sensitive to inhibition by n-butyl malonate, suggesting that the dicarboxylate exchange carrier was involved in the transport of malate out of the mitochondria. The capacity of the dicarboxylate shuttle to reduce NO,- was 63 % that of the in vitvo nitrate reductase activity in the supernatant
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1986 ◽
Vol 104
(3)
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pp. 367-372
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2017 ◽
Vol 07
(01)
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Keyword(s):
1979 ◽
Vol 25
(10)
◽
pp. 1169-1174
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