Bovine dominant follicular fluid promotes the in vitro development of goat preantral follicles

The aim of this study was to evaluate the effect of follicular fluid collected from bovine dominant follicles (bFF) on the in vitro development of goat preantral follicles and determine the best time to add this supplement to the culture medium. The preantral follicles were isolated and randomly distributed into four treatments in absence (control) or presence of 10% of bFF added on Days 0 (FF0–18), 6 (FF6–18) or 12 (FF12–18) of culture onwards. After 18 days, follicular development was assessed based on follicular survival, antral cavity formation, increased follicular diameter as well as fully grown oocyte (>110 μm) viability and meiosis resumption. The oocytes from the cultured follicles were in vitro-matured and processed for fluorescence or ultrastructural analysis. The results showed that on Day 18 the treatment FF0–18 had a significantly higher (P < 0.05) survival than control and FF12–18, but not FF6–18. The addition of bFF at the beginning of culture (FF0–18 and FF6–18) promoted a high percentage of follicular growth, meiosis resumption and early antrum formation. Moreover, this study described for the first time the ultrastructural analysis of caprine oocytes grown in vitro. This evaluation revealed that in the presence of bFF on (FF0–18) the in vitro-grown oocytes presented normal organelle distribution and well-defined, intact plasma and nuclear membranes. In conclusion, bFF originating from dominant follicles maintain the survival and promote the in vitro growth of goat preantral follicles when added at the beginning of culture.

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Effect of LH and Estradiol-17β Supplementation at Different Time Points on In vitro Development of Preantral Follicles in Sheep

Indian Journal of Animal Research ◽

10.18805/ijar.b-4119 ◽

2020 ◽

Author(s):

L.S.S. Varaprasad Reddy ◽

B.R. Naik ◽

A.V.N. Sivakumar ◽

B. Punyakumari ◽

J. Suresh

Keyword(s):

In Vitro Maturation ◽

Culture Medium ◽

Follicular Development ◽

Standard Medium ◽

In Vitro Development ◽

Preantral Follicles ◽

Time Points ◽

Vitro Development ◽

Estradiol 17Β

Background: Ovarian follicular development and growth are controlled by many hormones and growth factors. Despite the fact that LH and estradiol-17β have been utilized for the in vitro culture of preantral follicles yet, the suitable time points of supplementation of LH and estradiol-17β is not known. Therefore this study aimed to investigate the influence of addition of LH and estradiol-17β at different time points on in vitro development of preantral follicles (PFs’) in sheep. Method: Preantral follicles isolated from the ovarian cortical slices using micro dissection method were cultured for six days in Bicarbonate buffered Tissue culture medium 199B (TCM 199B) or in a standard culture medium supplemented with LH (2 μg/ml) and estradiol-17β (5 ng/ml) at different points during the culture period. COCs isolated from the follicles at the end of six day culture in different treatments were subjected to in vitro maturation for additional 24h. Result: Supplementation of LH and estradiol-17β during last two days of the culture supported better proportion of PFs’ exhibiting growth whereas supplementation of LH and estradiol-17β during first two days of the culture supported better average increase in diameter and proportion of PFs’ exhibiting antrum formation at the end of six day culture. Further the oocytes in COCs isolated at the end of culture in these treatments and subsequently subjected to in vitro maturation (IVM) for 24hr developed at a higher frequency to MII (metaphase II) stage. Supplementation of LH and estradiol-17β to TCM 199B culture medium in early stages followed by standard medium alone in later stages supports better development of PFs’ in vitro. Following supplementation with LH and estradiol-17β for the first two days culture of PFs’ in standard medium appears to be advantageous for the development of preantral follicles in vitro.

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Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

Theriogenology ◽

10.1016/j.theriogenology.2015.04.018 ◽

2015 ◽

Vol 84 (4) ◽

pp. 589-599 ◽

Cited By ~ 8

Author(s):

Elina V. García ◽

Dora C. Miceli ◽

Gabriela Rizo ◽

Pablo A. Valdecantos ◽

Antonio D. Barrera

Keyword(s):

Bone Morphogenetic Protein ◽

Embryo Culture ◽

Culture Medium ◽

Preimplantation Embryos ◽

In Vitro Development ◽

Morphogenetic Protein ◽

Embryo Culture Medium ◽

Vitro Development

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Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles

Theriogenology ◽

10.1016/j.theriogenology.2016.09.013 ◽

2017 ◽

Vol 88 ◽

pp. 124-130 ◽

Cited By ~ 5

Author(s):

Khairy M.A. Zoheir ◽

Gamaleldin I. Harisa ◽

Ahmed A. Allam ◽

Liguo Yang ◽

Xiang Li ◽

...

Keyword(s):

Lipoic Acid ◽

Alpha Lipoic Acid ◽

In Vitro Development ◽

Preantral Follicles ◽

Vitro Development

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Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Brazilian Journal of Medical and Biological Research ◽

10.1590/s0100-879x2010007500066 ◽

2010 ◽

Vol 43 (8) ◽

pp. 728-736 ◽

Cited By ~ 23

Author(s):

F.S. Martins ◽

J.J.H. Celestino ◽

M.V.A. Saraiva ◽

R.N. Chaves ◽

R. Rossetto ◽

...

Keyword(s):

Growth Hormone ◽

Growth Factor ◽

Insulin Like Growth Factor ◽

In Vitro Development ◽

Preantral Follicles ◽

Factor I ◽

Differentiation Factor ◽

Growth Differentiation Factor 9 ◽

Vitro Development

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In vitro development of morulae from immature caprine oocytes

Zygote ◽

10.1017/s0967199400001842 ◽

1994 ◽

Vol 2 (2) ◽

pp. 97-102 ◽

Cited By ~ 10

Author(s):

Levent Keskintepe ◽

Gamal M. Darwish ◽

Abdelmoneim I. Younis ◽

Benjamin G. Brackett

Keyword(s):

Oocyte Maturation ◽

Follicular Fluid ◽

Glycoprotein Hormones ◽

Glycoprotein Hormone ◽

In Vitro Development ◽

Morula Stage ◽

Vitro Development ◽

Hormone Control ◽

Medium Supplementation

SummaryThe effects of medium supplementation with oestrous goat serum and glycoprotein hormones on caprine oocyte maturation in vitro (IVM) were evidenced by proportions of resulting ova completing in vitro fertilisation (IVF) and development to the morula stage. Oocyte-cumulus complexes (OCCs) were harvested in follicular fluid from 2–5 mm diameter follicles. Oocyte maturation took place during 27 h in TCM-199 supplemented with 20% oestrous goat serum, oestradiol-17β (1.0 μg/ml), and either (a) 0.5 μg FSH/ml, (b) 100 μg LH/ml, (c) 100 μg LH + 0.5 μg FSH/ml, (d) 100 μg hCG + 0.5 μg FSH/ml, (e) 0.5 μg TSH/ml or (f) no added glycoprotein hormone (control). Of 353 immature oocytes cultured in seven experiments, 311 (88.1%) exhibited cumulus expansion at the end of the IVM interval; all normalappearing OCCs were inseminated. In vitro insemination was with ejaculated sperm treated with heparin (10 μg/ml) and caffeine (0.4 μg/ml). Proportions (%) of inseminated ova that were fertilised (cleaved) and that reached the morula stage after IVM with (a) FSH, (b) LH, (c) LH + FSH, (d) hCG + FSH, (e) TSH and (f) no added glycoprotein hormone were (a) 22/52 (42.3%) and 9/52 (17.3%), (b) 25/54 (46.3%) and 14/54 (25.9%), (c) 52/65 (80.0%) and 26/65 (40.0%), (d) 48/78 (61.5%) and 22/78 (28.2%), (e) 14/54 (25.9%) and 4/54 (7.4%), and (f) 11/50 (22.0%) and 1/50 (2.0%), respectively. All treatments yielded better results than IVM with no added glycoprotein hormone. After IVM with added LH + FSH higher proportions of oocytes were fertilised (p<0.05), and higher proportions reached the morula stage (p<0.05) when compared with other treatments.

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