290 OPTIMAL OF DOSE OF OVINE PITUITARY GLAND EXTRACT OVAGEN® FOR SUPERSTIMULATION OF BEEF COW DONORS IN ARGENTINA

2009 ◽  
Vol 21 (1) ◽  
pp. 242
Author(s):  
G. Brogliatti ◽  
J. Villarreal ◽  
L. Cutaia ◽  
A. Albrech ◽  
A. Garcia Guerra ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Total Dose ◽  
Embryo Transfer ◽  
Buenos Aires ◽  
Optimal Dose ◽  
Recommended Dose ◽  
Transfer Program ◽  
Embryo Viability ◽  
Gland Extract ◽  
Donor Cows

The success of an embryo transfer program is measured by the number of calves born alive by female donor in a given period. The success is influenced by several factors related to the number of ovulations, fertilization rate, and embryo viability (Armstrong D 1993 Theriogenology 39, 7–24). One of the main inconveniences of embryo transfer programs is the variability of superovulatory response to treatments. An experiment was designed to determine the optimal dose of ovine pituitary gland extract Ovagen® (ICPbio, Ltd., Auckland, New Zealand) for inducing superovulation in Aberdeen Angus donor cows. Sixty cycling multiparous donors with a condition score between 3.5 and 4.5 (Scale 1 to 5) were used. All donors received an intravaginal progesterone device DIB (1 g of Progesterone, Syntex, Buenos Aires, Argentina), along with 2 mg of EB (Estradiol Benzoate, Syntex, Buenos Aires, Argentina) and 50 mg of progesterone (Laboratorio Rio de Janeiro, Argentina) on Day 0. Superestimulatory treatment began on Day 4 and donors were randomly assigned to 3 treatment groups according to the total dose of NIADDK-oFSH-Z (Ovagen®) as follows: Group 1, cows received the total dose recommended by the manufacturer (100%* Group), 17.6 mg; Group 2, cows received 75% of the total dose recommended by manufacturer (75% Group), 13.2 mg and Group 3, cows received 50% of the total dose recommended by manufacturer (50% Group), 8.8 mg. All cows received two 150 μg of D+ cloprostenol IM (Ciclase, Syntex) injections on Day 6 given at 12 h interval. DIB was removed on Day 7 a.m. On Day 8 a.m., cows received 0.05 mg of GnRH IM (Gonasyn, Syntex). Fixed time AI was done on Day 8 p.m. and Day 9 a.m. with high quality frozen–thawed semen. On Day 15 embryo collection was performed by non-surgically method and evaluated by developmental stage and quality. The efficiency of superestimulatory response was evaluated by total amount of collected ova-embryos, fertilized ova and embryos Grade 1, 2, and 3, (according to IETS manual). One way AOV test was used to compare variables among groups and results are shown in Table 1. A significant increase in total CL was observed for the groups receiving 75% or 100% of the recommended dose with respect to the group receiving 50% of that dose. There was also a significant greater number of grade 1 embryos for the groups receiving 75% or 100% of the dose than in the group receiving 50% of the recommended dose. There were no differences for any of the evaluated parameters between 75% and 100% dose groups. These results suggest that acceptable superstimulatory responses can be obtained using reduced doses of Ovagen® in Aberdeen Angus donor cows. (*) the percentage makes reference to the total dose recommended by the laboratory of origin. Table 1.Embryo collection results of superstimulated donors with different dose* of NIADDK-oFSH-Z (Ovagen) Research supported by Syntex S.A. and Eolia S.A.

104 SOFaaci-HEPES or holding media can be used for embryo loading without changes in pregnancies per embryo transfer nor pregnancy loss in an invitro-produced embryo transfer program

2020 ◽  
Vol 32 (2) ◽  
pp. 178
Author(s):  
D. Pereira ◽  
D. Moreno ◽  
R. Sala ◽  
L. Carrenho-Sala ◽  
M. Fosado ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Fixed Effects ◽  
Pregnancy Loss ◽  
Embryo Quality ◽  
Prostaglandin F2α ◽  
P Value ◽  
Transfer Program ◽  
Embryo Viability ◽  
Stage Embryo ◽  
Embryo Stage

Time elapsed between removal from culture and embryo transfer (ET) can have a profound effect on the success of an invitro-produced (IVP) ET program. The embryo culture medium provides the necessary nutrients for embryo development and the use of media with a different nutrient composition to load embryos into straws could negatively affect embryo viability. The objective of the present study was to evaluate the effect of type of media used for embryo loading on pregnancy establishment and maintenance. Holstein heifers (n=800) were synchronized using a modified 5-day CO-Synch + controlled internal drug release (CIDR) as follows: Day −8: CIDR inserted, Day −3: CIDR removed, prostaglandin F2α treatment (500μg cloprostenol sodium), Day 0: gonadotrophin-releasing hormone (GnRH; 100μg of gonadorelin acetate). Five days after GnRH, heifers were evaluated by ultrasonography to determine presence of a corpus luteum (CL). Embryos were removed from culture on Day 7 (Day 0=fertilization), placed into tubes containing SOFaaci, and transported in an incubator (LabMix, WTA) to the transfer facility within 1.5h. Upon arrival embryos were removed from transport tubes and randomly assigned to be loaded into 0.25-mL straws containing either holding media (Vigro Holding Plus) or SOFaaci-HEPES. After loading into straws, embryos were placed in an ET gun and AI gun warmers set at 35°C until transfer by 1 of 5 technicians. Heifers with a CL were randomised for transfer of a fresh IVP embryo loaded into a straw containing either holding media or SOFaaci-Hepes on Day 7±1. Interval from embryo loading to transfer ranged from 1 to 3h. Pregnancy was determined by ultrasonography on Days 32 and 60. Data were analysed by logistic regression and included the fixed effects of loading media, embryo stage, embryo quality, interval between GnRH and ET, and biologically relevant interactions. Pregnancies per ET (P/ET) on Day 32 were not different between the groups in which embryos were loaded using holding media and those which used SOFaaci-Hepes, nor there were interactions between loading medium and embryo stage, embryo quality, or interval from GnRH to ET (P>0.10; Table 1). Pregnancies per ET (P/ET) on Day 60 were not different between the loading media groups, nor were there interactions between loading medium, embryo stage, and embryo quality, or interval from GnRH to ET (P>0.10). Pregnancy loss between Days 32 and 60 was not different between groups, nor there were interactions between loading media groups and any other factor (P>0.10). In conclusion, the use of either holding medium or SOFaaci-HEPES for fresh IVP embryo loading does not affect fertility; thus, both are suitable alternatives for loading of embryos into transfer straws. Table 1.Pregnancies per embryo transfer (P/ET) and pregnancy loss in recipient heifers transferred with fresh invitro-produced embryos, using either holding medium or SOFaaci-HEPES medium for loading Item P/ET Day 32 (n) P/ET Day 60 (n) Pregnancy loss (n) Loading medium Holding 47.0% (186/396) 41.3% (163/395) 11.9% (22/185) SOFaaci-HEPES 48.8% (197/404) 43.1% (174/404) 11.7% (23/197) P-value 0.77 0.22 0.84

384 EFFECTS OF CONDITION SCORE ON OVARIAN RESPONSE AND TRANSFERABLE EMBRYOS IN SUPERSTIMULATED COWS IN ARGENTINA

2007 ◽  
Vol 19 (1) ◽  
pp. 307 ◽  
Author(s):  
A. Garcia Guerra ◽  
D. Rodriguez ◽  
J. Villareal ◽  
A. Albrecht ◽  
G. Brogliatti
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Transrectal Ultrasound ◽  
Ovarian Response ◽  
Estradiol Benzoate ◽  
Buenos Aires Province ◽  
Transfer Program ◽  
Embryo Viability ◽  
Significant Difference ◽  
Condition Score

The success of an embryo transfer program is influenced by ovarian response, number of ovulations, fertilization, and embryo viability (Armstrong 1993). It has been observed that over-conditioned, overweight donor females tend to produce fewer viable embryos per collection (Stroud and Hasler 2006 Theriogenology 65, 65–76). The condition score is one of the many intrinsic factors that affects cow reproduction. A retrospective analysis was done to compare the ovarian response, number of ovulations, fertilization, and total transferable embryos recovered of cows with different condition scores in Argentina. This research was carried out using different breed donors (n = 640; 82% Angus; 18% other) during the last 4 years in Buenos Aires province, Argentina. Donors at random stages of follicular wave with CL received an intravaginal progesterone device (DIB; Syntex, Buenos Aires, Argentina), 2 mg of estradiol benzoate, and 50 mg of progesterone (Syntex, Buenos Aires, Argentina) IM on the same day. On Day 4 after DIB insertion, superestimulatory treatment was initiated on a decreasing dose regimen of FSH (Pluset®; Callier, São Paulo, Brazil, or Folltropin®; Bioniche Animal Health, Inc., Belleville, Ontario, Canada) as IM injections every 12 h during 4 days. On Day 6, DIBs were removed, and cows received a double dose of 2 mL of cloprostenol within 12 h. At heat detection, all donors received a dose of 2 mL of GnRH (Dalmarelin; Von Franken, Buenos Aires, Argentina) by IM injection and were inseminated 12 and 24 h thereafter. Seven days later, embryo collection was performed and ovarian response was evaluated as number of CL + anovulatory follicles by transrectal ultrasound using a 7.5-MHz transducer (Pie Medical, Maastricht, The Netherlands). The condition score was determined at this time using a 1 to 5 scale. Embryos recovered were evaluated and classified according to the IETS manual. Kruskal–Wallis test was used to compare variables among score groups, and results are shown in Table 1. Ovarian response as the number of CL and anovulatory follicles was significantly different (P = 0.0001) among groups. There is a negative correlation (−0.2194) between condition score and ovarian response. At higher condition scores, ovarian response decreased. There is a significant difference (P = 0.0001) for the number of ovulations and total number of fertilized ova among groups. Finally, the total number of transferable embryos was no different for either score; however, there is a trend toward decrease when the condition score is higher. In conclusion, these results indicate that using donors with condition scores of 2 to 3 improves the results of a superstimulatory treatment. Controlling donors' condition score by nutrition management gives us the possibility to produce more embryos per donor treatment. Table 1. Condition score, ovarian response, fertilization, and transferable embryos in donor cows in Argentina

The effect of endometriosis on implantation: results from the Yale University in vitro fertilization and embryo transfer program

1996 ◽  
Vol 65 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Aydin Arici ◽  
Engin Oral ◽  
Orhan Bukulmez ◽  
Antoni Duleba ◽  
David L. Olive ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Transfer Program ◽  
Yale University ◽  
Vitro Fertilization ◽  
Embryo Transfer Program

The in vitro fertilization and embryo transfer program at the first Women's Hospital ?T. Kirkova,? Sofia, Bulgaria

1990 ◽  
Vol 7 (2) ◽  
pp. 119-119 ◽  
Author(s):  
Ilya Vatev ◽  
Philip Fitchev ◽  
Pavleta Tabakova ◽  
Mitko Dimitrov ◽  
Tania Yanakieva ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Transfer Program ◽  
Vitro Fertilization ◽  
Embryo Transfer Program

Human Immune Serum Globulins

1983 ◽  
Vol 4 (10) ◽  
pp. 333-333
Keyword(s):  
Hepatitis B ◽  
Total Dose ◽  
Immune Globulin ◽  
Immune Serum ◽  
Recommended Dose ◽  
Hepatitis B Immune Globulin ◽  
Human Immune

In the November issue of PIR (4:135, 1982), on p 140 under "Hepatitis B Immune Globulin (HBIG), Recommendation," the next to the last paragraph states: "In newborns with a possible exposure to hepatitis B, a dose of 0.5 ml/kg of HBIG is recommended." This recommended dose is much too large for a newborn. The dose recommended is a total dose of 0.5 ml IM, to be given as soon after birth as possible (no later than 24 hours). The same dose (0.5 ml) should be repeated three months and six months later.

First field results on the use of stallion sex-sorted semen in a large-scale embryo transfer program

2014 ◽  
Vol 81 (4) ◽  
pp. 520-525 ◽  
Author(s):  
M. Panarace ◽  
R.O. Pellegrini ◽  
M.O. Basualdo ◽  
M. Belé ◽  
D.A. Ursino ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Large Scale ◽  
Transfer Program ◽  
Embryo Transfer Program
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