384 EFFECTS OF CONDITION SCORE ON OVARIAN RESPONSE AND TRANSFERABLE EMBRYOS IN SUPERSTIMULATED COWS IN ARGENTINA

2007 ◽  
Vol 19 (1) ◽  
pp. 307 ◽  
Author(s):  
A. Garcia Guerra ◽  
D. Rodriguez ◽  
J. Villareal ◽  
A. Albrecht ◽  
G. Brogliatti
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Transrectal Ultrasound ◽  
Ovarian Response ◽  
Estradiol Benzoate ◽  
Buenos Aires Province ◽  
Transfer Program ◽  
Embryo Viability ◽  
Significant Difference ◽  
Condition Score

The success of an embryo transfer program is influenced by ovarian response, number of ovulations, fertilization, and embryo viability (Armstrong 1993). It has been observed that over-conditioned, overweight donor females tend to produce fewer viable embryos per collection (Stroud and Hasler 2006 Theriogenology 65, 65–76). The condition score is one of the many intrinsic factors that affects cow reproduction. A retrospective analysis was done to compare the ovarian response, number of ovulations, fertilization, and total transferable embryos recovered of cows with different condition scores in Argentina. This research was carried out using different breed donors (n = 640; 82% Angus; 18% other) during the last 4 years in Buenos Aires province, Argentina. Donors at random stages of follicular wave with CL received an intravaginal progesterone device (DIB; Syntex, Buenos Aires, Argentina), 2 mg of estradiol benzoate, and 50 mg of progesterone (Syntex, Buenos Aires, Argentina) IM on the same day. On Day 4 after DIB insertion, superestimulatory treatment was initiated on a decreasing dose regimen of FSH (Pluset®; Callier, São Paulo, Brazil, or Folltropin®; Bioniche Animal Health, Inc., Belleville, Ontario, Canada) as IM injections every 12 h during 4 days. On Day 6, DIBs were removed, and cows received a double dose of 2 mL of cloprostenol within 12 h. At heat detection, all donors received a dose of 2 mL of GnRH (Dalmarelin; Von Franken, Buenos Aires, Argentina) by IM injection and were inseminated 12 and 24 h thereafter. Seven days later, embryo collection was performed and ovarian response was evaluated as number of CL + anovulatory follicles by transrectal ultrasound using a 7.5-MHz transducer (Pie Medical, Maastricht, The Netherlands). The condition score was determined at this time using a 1 to 5 scale. Embryos recovered were evaluated and classified according to the IETS manual. Kruskal–Wallis test was used to compare variables among score groups, and results are shown in Table 1. Ovarian response as the number of CL and anovulatory follicles was significantly different (P = 0.0001) among groups. There is a negative correlation (−0.2194) between condition score and ovarian response. At higher condition scores, ovarian response decreased. There is a significant difference (P = 0.0001) for the number of ovulations and total number of fertilized ova among groups. Finally, the total number of transferable embryos was no different for either score; however, there is a trend toward decrease when the condition score is higher. In conclusion, these results indicate that using donors with condition scores of 2 to 3 improves the results of a superstimulatory treatment. Controlling donors' condition score by nutrition management gives us the possibility to produce more embryos per donor treatment. Table 1. Condition score, ovarian response, fertilization, and transferable embryos in donor cows in Argentina

295 SUPEROVULATORY RESPONSE IN COWS FOLLOWING SYNCHRONIZATION OF FOLLICLE WAVE EMERGENCE WITH ESTRADIOL AT DIFFERENT STAGES OF THE ESTROUS CYCLE

2008 ◽  
Vol 20 (1) ◽  
pp. 227
Author(s):  
A. Garcia Guerra ◽  
G. A. Bó ◽  
J. Villarreal ◽  
G. M. Brogliatti
Keyword(s):  
Estrous Cycle ◽  
Buenos Aires ◽  
Animal Health ◽  
Ovarian Response ◽  
Buenos Aires Province ◽  
Transfer Program ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Significant Difference ◽  
Group 2

Ovarian asynchrony and variability in response to superstimulation remain the most limiting factors in any embryo transfer program (Armstrong D 1993 Theriogenology 39, 7–24). Ovarian response can be increased and less variable if superstimulatory treatment is started at the time of follicular wave emergence (Bö GA et al. 1995 Theriogenology 43, 31–40). A combination of progesterone (P4) and estradiol have been used to synchronize follicular wave for superstimulation. A retrospective analysis was done to compare the ovarian response, superovulatory response and embryo production of cows in Argentina that received progesterone and estradol prior to superstimulation at different stages of the estrous cycle. This research was carried out using different breed of donors (n = 584, 88% Angus) during the last 4 years in Buenos Aires province, Argentina. Heat detection was performed twice a day. At random stages of the estrous cycle, donors received an intravaginal progesterone device (DIB; Syntex, Buenos Aires, Argentina), 2 mg of estradiol benzoate and 50 mg of progesterone (Syntex, Buenos Aires, Argentina) IM on the same day. On day 4 after DIB insertion, superestimulatory treatment was initiated on a decreasing dose regimen of FSH (Pluset; Callier, Spain, or Folltropin, Bioniche Animal Health Inc., Belleville, Ontario, Canada) as IM injections every 12 h over 4 d. On day 6, DIBs were removed, and cows received two doses of 2 mL of cloprostenol 12 h apart. At heat detection, all donors received a dose of 2 mL of GnRH (Dalmarelin; Fatro Von Franken, Buenos Aires, Argentina) by IM injection and were inseminated 12 and 24 h later. Seven days later, embryo collection was performed and ovarian response was evaluated as number of CL + unovulated follicles by transrectal ultrasound using a 7.5-MHz transducer (Pie Medical, Maastricht, the Netherlands). Ova/embryos were evaluated and classified according to the IETS manual. Donors were assigned to receive DIB and estradiol during the following stages of the cycle: group 1: between days 4 and 7 post-estrus (dominant follicle period), group 2: between days 8 and 12 post-estrus (emergence of the second follicular wave), and group 3: between days 13 and 21 post-estrus (dominant follicle of the second wave). Kruskal-Wallis test was used to compare variables among groups, and results are shown in Table 1. Ovarian response as CL + unovulated follicles and number of ovulations were significantly different among groups (P < 0.05). However, there was no significant difference in the number of fertilized ova or transferable embryos. Nevertheless, numeric differences that show that group 2 (started between days 8 and 12 post-estrus) was always superior for all variables. In conclusion, data suggest that estradiol may be more effective in synchronizing follicle wave emergence for superstimulation during the mid-part of the estrous cycle. Table 1. Superovulatory response in cows in which follicle wave emergence was synchronized with estradiol at different stages of the estrous cycle (mean ± SD) Research supported by Centro Genetico Bovino Eolia S.A.

409 SUPERSTIMULATED BOVINE DONORS INSEMINATED WITH SEMEN WITH DIFFERENT LINEARITY EVALUATED BY COMPUTER-ASSISTED SPERM ANALYSIS

2010 ◽  
Vol 22 (1) ◽  
pp. 361
Author(s):  
A. Garcia Guerra ◽  
J. Villareal ◽  
G. Larraburu ◽  
G. M. Brogliatti
Keyword(s):  
Buenos Aires ◽  
Semen Quality ◽  
Present Report ◽  
Animal Health ◽  
Computer Assisted ◽  
Buenos Aires Province ◽  
Sperm Analysis ◽  
Significant Difference ◽  
Computer Assisted Sperm Analysis ◽  
Group 1

There is a highly significant relationship between semen quality and the percentage of fertilized ova and transferable embryos in superovulated donors (Stroud B and Hasler JF 2006 Theriogenology 65, 65-76). Computer-assisted sperm analysis (CASA) provides an objective and highly repeatable system for sperm analysis. It has been observed that linearity (LIN) measured by CASA has a high correlation with fertility (Foote RH et al. 1998 Theriogenology 49, 871-879). In the present report, retrospective analysis was done to determine the effect of sperm linearity as assessed by CASA on the number of ova and viable embryos recovered from superstimulated cows. This research was carried out using different breeds of donors (n = 150, 80% Angus) during the last 5 years in an embryo transfer center in Buenos Aires province, Argentina. Donors with a CL received an intravaginal progesterone device (DIB®, Syntex, Buenos Aires, Argentina), 2 mg of 17? estradiol, and 50 mg of progesterone (Rio de Janeiro, Brazil), i.m. on the same day. On Day 4 after DIB® insertion, superstimulatory treatment was initiated as a decreasing dose regimen of FSH i.m. (Pluset®, Callier, Spain, or Folltropin®, Bioniche Animal Health, Inc., Belleville, Ontario, Canada) every 12 h during 4 days. On Day 6, DIB® devices were removed and cows were administered cloprostenol (2 mL) twice at 12-h intervals. When estrus was detected, donors received GnRH (2 mL i.m. Dalmarelin®, Von Franken, Buenos Aires, Argentina) and were inseminated 12 and 24 h thereafter. Semen was thawed in a water bath at 37°C for 1 min. Before insemination, every semen dose was evaluated for motility characteristics using the IVOS Sperm Analyzer (Hamilton Thorne Biosciences, Beverly, MA, USA). Two chambers of 20-(im depth and 5 fields per chamber were analyzed (30 frames/0.5 s for each field). According to CASA results, 3 groups were defined based on LIN: Group 1 = <50%; Group 2 =≥50% and <60%; and Group 3 =≥60%. Seven days later, embryo collection was performed and ovarian response was evaluated by transrectal ultrasonography to assess number of CL + anovulatory follicles. Ova/embryos were evaluated and classified according to the IETS manual. Kruskal-Wallis test was used to compare variables among groups, and results are shown in Table 1. There was a significant difference between Groups 1 and 3 for total number and percentage of viable embryos. More embryos were recovered from donors inseminated with higher LIN semen. Also, there was a significant difference between Group 1 and both Groups 2 and 3 in the number of total fertilized ova, percentage of fertilized ova, and percentage of unfertilized ova. The present results suggest that insemination of donors with semen of high LIN results in higher fertilization rates and number of viable embryos. Table 1.Number of fertilized ova and viable embryos of donor cows inseminated with semen with different linearity

scholarly journals Capture efficiency for small dominant mesozooplankters (Copepoda, Appendicularia) off Buenos Aires Province (34ºS-41ºS), Argentine Sea, using two plankton mesh sizes

2009 ◽  
Vol 57 (3) ◽  
pp. 205-214 ◽  
Author(s):  
Rosana Di Mauro ◽  
Fabiana Capitanio ◽  
María Delia Viñas
Keyword(s):  
Buenos Aires ◽  
Developmental Stages ◽  
Fish Larvae ◽  
Abundant Species ◽  
Accurate Estimation ◽  
Buenos Aires Province ◽  
Oikopleura Dioica ◽  
Great Loss ◽  
Argentine Sea ◽  
Significant Difference

Two plankton mesh sizes of 67 µm and 220 µm were compared to evaluate their efficiency in the capture of the smallest copepods and appendicularians present in the Buenos Aires coastal area (Argentine Sea). A total of 12 copepod species and one appendicularian species were recorded in this study. The copepods were separated into 4 groups: harpacticoids, cyclopoids, small calanoids and large calanoids and their developmental stages. Among the cyclopoids, Oithona nana was the most abundant species, being 96.29 % underestimated by the 220 µm mesh, whereas Microsetella norvegica dominated the harpacticoids and was captured exclusively by the smaller mesh. Similar results were found for copepodites I-III of small calanoids, whose net sampled underestimation reached 99.70%. On the other hand, no significant difference between meshes was found for adults and copepodites IV-V of small calanoids or any of the developmental stages of large calanoids. A great loss of biomass was observed for O. nana when applying the larger mesh. In regards to the appendicularian Oikopleura dioica, all size ranges below 1,000 µm length were better estimated by the 67 µm mesh in terms of abundance and biomass. Our results clearly show that the 67 µm mesh was more efficient in the capture of early stages of small copepods thus providing a more accurate estimation of the fish larvae prey field.

151 EFFECT OF RECIPIENT CATEGORY ON PREGNANCY RATES ON A BOVINE EMBRYO TRANSFER PROGRAM IN PATAGONIA, ARGENTINA

2008 ◽  
Vol 20 (1) ◽  
pp. 155
Author(s):  
J. Villarreal ◽  
A. Garcia Guerra ◽  
G. M. Brogliatti
Keyword(s):  
Embryo Transfer ◽  
Pregnancy Rates ◽  
Point Of View ◽  
Bovine Embryo ◽  
Transfer Program ◽  
Chi Square ◽  
Lactating Cows ◽  
Significant Difference ◽  
Embryo Transfer Program ◽  
Condition Score

The fertility of the recipient is one of the more important factors conditioning the success of an embryo transfer (ET) program. Selection and management of recipients is a very important contributing factor to achieve high pregnancy rates (Stroud and Hasler 2006 Theriogenology 65, 65–76). From a reproductive point of view, a good recipient is a cow capable of receiving an embryo and taking it to term (Palma et al. 2001 Biotecnologia de la reproduccion, INTA). In beef and dairy cattle, recipients are categorized according to number of births, age, and condition score (Stroud and Hasler 2006). A retrospective analysis was done to confirm a relationship between recipient category, based on their physiologic and reproductive stage, and pregnancy rates. The analysis was done during January and February (2006–2007) based on data recovered from an embryo transfer program done in Chubut province (Patagonia) for the project ‘Polo Genetico Angus’ (Angus Association – Chubut government agreement). Two hundred and seven morulas and/or blastocysts, quality 1 or 2 frozen in 1.5 m ethylene glycol (IETS manual), were transferred randomly in 10 different farms. Embryos were thawed for 10 s at room temperature and 30 s in a water bath at 35°C and transferred by one single technician. Angus, Hereford, or its breeds were used as recipients. A reproductive exam was performed before embryo transfer and, based on it, recipients were classified into 4 categories: heifer (3–4 years old), lactating cow, weaned cow, and dry old cow (Table 1). The synchronization protocol used for recipients consisted of a single administration of D-cloprostenol and heat detection twice a day for 5 days. Data were analyzed by Cochran-Mantel-Haenszel test (chi-square). Results are shown in Table 1. There is a significant difference (P ≤ 0.05) in pregnancy rates among categories. Recipients with higher pregnancy rates were lactating cows and weaned cows 61.6% and 56.9%, respectively, compared to heifers, 45.3%; a lower pregnancy rate was founded in the dry old cows group. Dry cows were animals that failed to get pregnant during the last season. Heifers also were animals that failed the artificial insemination program. In both groups, the reduced fertility may explain their lower pregnancy rates. In conclusion, selection toward fertility of recipients is a very important factor to take into account to design a successful embryo transfer program. Table 1. Pregnancy rates for recipient categories in an ET program in Chubut (Patagonia), Argentina This research was supported by Centro Genetico Bovino Eolia.

202 ANTRAL FOLLICULAR COUNTS AND SUPERSTIMULATORY RESPONSE IN PREPUBERTAL CALVES

2017 ◽  
Vol 29 (1) ◽  
pp. 210
Author(s):  
A. R. T. Krause ◽  
F. C. F. Dias ◽  
G. Adams ◽  
R. Mapletoft ◽  
W. F. Huanca ◽  
...  
Keyword(s):  
Natural Science ◽  
Animal Health ◽  
Transrectal Ultrasound ◽  
Correlation Coefficients ◽  
Ovarian Response ◽  
Science And Engineering ◽  
Follicular Wave ◽  
Video Recordings ◽  
Oocyte Collection ◽  
Science Without Borders

The number of follicles recruited in successive waves are consistent in postpubertal cattle (Singh et al. 2004 Theriogenology 62, 227), but ovarian response to gonadotropin stimulation is highly variable among animals. We tested the hypotheses that the number of follicles present at wave emergence are repeatable and are predictive of superstimulatory response in prepubertal calves; therefore, we expected that calves with higher antral follicular counts (AFC; follicles = 1mm) at wave emergence will result in a greater number of follicles available for oocyte collection after a conventional 4-day gonadotropin treatment. Hereford crossbreed calves (n = 52; 5.1 to 6.8 months of age) were ranked according to the number of follicles = 1 mm detected in transrectal ultrasound video recordings of both ovaries at the time of wave emergence (First AFC; range: 12 to 53 follicles). Calves in the bottom (Low AFC; <20 follicles; n = 6) and top (High AFC; >32 follicles; n = 5) quartiles were selected for ovarian superstimulation. Emergence of a new follicular wave (Day 0) was induced by transvaginal follicle ablation (14 to 57 days after first AFC; 5.7 to 7.1 months of age), AFC were performed again (Second AFC), and calves were given eight 12-hourly IM injections of 25 mg of pFSH (Folltropin-V®, Bioniche Animal Health Inc., Belleville, Canada) beginning on Day 0.5. All calves were given 12.5 mg of pLH (Lutropin-V®, Bioniche Animal Health Inc.) IM 12 h after the last FSH and number of follicles equal to 3, 3 to 5, 6 to 8, and equal to 6 and 9 were counted 24 h after LH treatment (at the time of oocyte collection). A t-test was used to compare the number of follicles and ovarian response (Low v. High AFC). Values of Pearson (0.8; P < 0.001) and Spearman (0.9; P < 0.001) correlation coefficients between First and Second AFC indicate strong repeatability of numbers of follicles present at the time of wave emergence. As expected, mean number of follicles were greater (P = 0.01) in the High- than Low-AFC group (24.2 ± 2.0 v. 15.7 ± 1.0) at the Second AFC. The High-AFC group had a greater number of follicles at oocyte collection than Low AFC for 6 to 8 mm (13.4 ± 2.1 v. 5.3 ± 1.7; P = 0.01), but not for 3 to 5 mm (9.4 ± 2.5 v. 5.3 ± 2.1; P = 0.2) or 9 mm (7.6 ± 2.9 v. 4.8 ± 2.0; P = 0.4) size categories. However, High AFC resulted in a greater total number of follicles 3 (30.4 ± 3.1 v. 15.5 ± 3.2; P = 0.009) and 6 mm (21.0 ± 4.1 v. 10.2 ± 2.9; P = 0.05). The number of 6-mm follicles at the end of superstimulation represented 80 and 60% of 1-mm follicles at wave emergence in the High- and Low-AFC groups (P = 0.3). In conclusion, the number of follicles at the beginning of a wave are predictive of follicles recruited into subsequent waves in 7-month-old calves, and higher AFC at wave emergence resulted in a greater number of 3- and 6-mm follicles available for oocyte collection. Research was supported by the Natural Science and Engineering Council of Canada (NSERC). Hormones provided by Vetoquinol Inc. ARTK funded by CNPq-Science Without Borders, Brazil.

Effect of different doses of FSH on superovulation, productionand quality of embryo in North Omani Cattle breed

2017 ◽  
Author(s):  
Y.O. Muhammad Al-Shakaili ◽  
A.M. Hussein ◽  
A.N. Al-Ismaily ◽  
H.H. Al-Alawi
Keyword(s):  
Embryo Quality ◽  
Follicle Stimulating Hormone ◽  
Cattle Breed ◽  
Ovarian Response ◽  
Transfer Program ◽  
Significant Difference ◽  
Embryo Transfer Program ◽  
Fair Poor ◽  
Different Doses

The experiment was conducted to investigate the Ovarian response and embryo quality using different doses of follicle stimulating hormone (FSH-P) in North Omani Cattle breed (NOC) in order to determine the suitable dose. Eighteen donor NOC were divided into three groups (G1, G2, and G3) comprising of six cows in each group and treated with 20 (G1), 25 (G2) and 30 mg FSH (G3). Ovarian response was recorded as development of corpus luteum (CL), unovulated follicles (UOF), unfertilized ova (UFO), recovered embryo (RE) and transferable embryos (TE). Embryos were flushed seven days after the first AI and classified according to the developmental stage and quality. Results showed that, cows superovulated with FSH (G3) increased significantly (P less than 0.05) number of CLs and number of RE (6.83 and 5.33 /flushed cow) compared with the G1 of FSH (3.17 and 2.00/flushed cow) and G2 (4.67 and 3.00/flushed cow). While using FSH in (G2) increased significantly (P less than 0.05) the number of TE (excellent and good) quality, decreased average number of UOF and UFO (2.84, 3.33 and 0.67/flush) compared with FSH G3 (1.83, 5.67 and 1.50/flush). While no significant difference was observed in TE number between G1 and G2. G3 groups producing embryos at early stages of development (4 and 8 cells) and this was undesirable for embryo transfer program as this treatment (30 mg) produced more number of fair, poor and degenerated embryos compared with 20 and 25 mg FSH. Consequently, 25 mg FSH dose increased number of TE, decreased number of UOF and UFO, and that was the suitable dose for superovulation of NOC.

32 USE OF HORMONE WITH ACTION FOLLICLE-STIMULATING IN OVULATION SYNCHRONIZATION OF CROSSBRED HOLSTEIN-GYR COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 174
Author(s):  
V. A. Nascimento ◽  
G. S. Moura ◽  
M. Dias ◽  
F. A. Oliveira ◽  
A. R. Dias ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Body Condition ◽  
Buenos Aires ◽  
Body Condition Score ◽  
Animal Health ◽  
Live Weight ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
São Paulo

The objective of this study was to evaluate the administration of the follicle-stimulating hormone (FSH-p) or equine chorionic gonadotropin (eCG) in protocols of ovulation synchronization. One hundred forty-nine crossbred Holstein-Gyr cows were used in 2 properties in Minas Gerais and 3 treatments: TControl (n = 49 cows), Day 0: insert of a progesterone device (Primer®, Tecnopec, São Paulo, Brazil) + 2 mg of estradiol benzoate (RIC-BE®); Day 8: removal of Primer + 112 mg of D-cloprostenol (Prolise®, ARSA SRL, Buenos Aires, Argentina) + 1 mg of estradiol benzoate and fixed-time artificial insemination (FTAI) accomplished 44 h after the removal of Primer; TFSH (n = 50 cows), similar to TControl, but on Day 8, 15 mg of FSH-p (Folltropin®, Bioniche Animal Health) was administered; TeCG (n = 50 cows), similar to TControl, with administration on Day 8 of 300 IU of eCG (Novormon®, Syntex SA, Buenos Aires, Argentina). Cows that returned to estrus 14 to 28 days after the FTAI were inseminated once again. For all statistical analysis, we used the program SAS 9.0 (2002; SAS Institute Inc., Cary, NC, USA) at 5% probability. Quantitative variables (live weight, days postpartum, follicular average per cow) were analyzed by variance, and body condition score by Friedman test. The pregnancy rates were submitted to logistic analysis considering the effect of local experimental factors and were analyzed by Spearman correlation among the variables studied and the pregnancy rate. There was no difference (P > 0.05) between treatment for pregnancy rate in FTAI protocols (TControl = 40.82%; TFSH = 38.00%; TeCG = 26.00%), in the first insemination after FTAI (TControl = 56.25%; TFSH = 70.59%; TeCG = 68.42%), and in FTAI plus first insemination after FTAI (TControl = 59.18%; TFSH =62.00%; TeCG = 52.00%), as well as for the estrus repetition (TControl = 55.17%; TFSH = 54.84%; TeCG = 51.35%) and the correlation (P > 0.05) among the pregnancy rate and postpartum period, milk production, body weight and body condition. No effect was observed on the pregnancy rate by the addition of the hormones FSH and eCG in the protocol of ovulation synchronization in crossbred Holstein-Gyr cows; however, reinsemination in the period from 14 to 28 days after FTAI increased the pregnancy rate. CNPq; FAPEMIG; TECNOPEC/São Paulo, Brazil.

402 SUPEROVULATION OF TROPICAL-ADAPTED BOS TAURUS AND BOS INDICUS COWS IN A TROPICAL ENVIRONMENT

2010 ◽  
Vol 22 (1) ◽  
pp. 357
Author(s):  
R. H. Alvarez ◽  
A. C. Martinez ◽  
R. M. L. Pires
Keyword(s):  
Ovarian Function ◽  
Bos Taurus ◽  
Animal Health ◽  
Bos Indicus ◽  
Ovarian Response ◽  
Estradiol Benzoate ◽  
Maximum Temperature ◽  
Embryo Production ◽  
Chi Square ◽  
Average Maximum

Breed differences in ovarian function were found among beef Bos indicus and Bos taurus cows maintained in a subtropical environment (Alvarez P et al. 2000 J. Anim. Sci. 78, 1291-1302). The aim of this study was to compare ovarian response to superovulation and embryo production of tropical-adapted Bos taurus and Bos indicus cows. The experiment was carried out in a tropical wet climate at the experimental station of Instituto de Zootecnia (latitude 22°46′S, longitude 47°17′W) from November to February (average maximum temperature = 30.0 ± 0.8°C and average absolute precipitation = 153.1 ± 78.8 mm3). Forty Caracu (a local Bos taurus breed) and 50 Nelore (Bos indicus breed) lactating cows were treated with an intravaginal device containing progesterone (1.38 mg; CIDR-B®, Pfizer Animal Health, Montreal, Québec, Canada) and 2.5 mg i.m. of estradiol benzoate (Estrogin®, Farmavet, São Paulo, Brazil). Four days later, the animals were superovulated with multiple i.m. injections of 400 IU of FSH (Pluset®, Calier, Spain) in decreasing doses (75-75, 75-50, 50-25, and 25-25 IU) at 12-h intervals over 4 days. The CIDR-B® device was removed 3 days after the first superovulatory injection and cows received i.m. 150 μg of cloprostenol (Veteglan®, Calier, Spain). Cows were inseminated 48 and 62 h after the cloprostenol injection and embryos were recovered nonsurgically 7 days after insemination. Differences in the number of CL (assessed by ultrasound scanning), total number of ova/embryos, and number of transferable embryos were analyzed by ANOVA. Differences in the number of animals with low response (<3 CL) to superovulation were analyzed by chi-square test. All donors (with the exception of 1 Caracu and 2 Nelore) with ovarian response >3 CL showed estrus at insemination. Three (8.9%) Caracu and 5 (10.0%) Nelore cows had <3 CL following the superovulation treatment (P = 0.68). There was no difference (P > 0.05) in the mean (± SEM) CL counts of Caracu (11.4 ± 3.3) and Nelore (12.0 ± 4.1) cows. Similarly, there were no differences (P > 0.05) between Caracu and Nelore cows for total number of ova/embryos collected (8.6 ± 2.6 v. 9.0 ± 4.3) or transferable embryos (6.0 ± 2.4 v. 5.1 ± 2.9). In conclusion, the superovulation of Caracu and Nelore cows carried out in a tropical climate resulted in similar ovarian responses and embryo production. Supported by FAPESP.

224 EFFECT OF LENGTHENING THE SUPERSTIMULATORY TREATMENT PROTOCOL ON THE OVARIAN RESPONSE AND NUMBERS OF TRANSFERABLE EMBRYOS IN BEEF COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 224
Author(s):  
A. Garcia Guerra ◽  
A. Tribulo ◽  
J. Yapura ◽  
J. Singh ◽  
R. J. Mapletoft
Keyword(s):  
Total Dose ◽  
Collection Efficiency ◽  
Animal Health ◽  
Treatment Protocol ◽  
Prostaglandin F2α ◽  
Ovarian Response ◽  
Beef Cows ◽  
Control Group ◽  
Corpora Lutea ◽  
Significant Difference

The present study was designed to test the hypothesis that an increase in the length of exposure of ovulatory follicles to progesterone and FSH during ovarian superstimulation will increase the number of ovulations and viable embryos in cattle. Twenty-four beef cows were initially subjected to follicle ablation at random stages of the oestrous cycle to determine the number of follicles at wave emergence; cows with comparable numbers of follicles were randomly allocated to groups by replicate. A single dose of prostaglandin F2α (PGF; Estroplan, Vétoquinol, QC, Canada) was given IM 7 to 9 days after follicle ablation and daily ultrasound examinations were performed to detect ovulation. Follicular ablation of all follicles ≥5 mm was done 5 to 8 days after ovulation and a progesterone-releasing intravaginal device (PRID, Vétoquinol) was inserted. The Control group (n = 12) was treated with 4 days of FSH and cows in the Long group (n = 12) were given 7 days of FSH treatment. The FSH treatments were initiated 1.5 days after ablation (Day 0) with a total dose of 400 mg of NIH-FSH-P1 (Folltropin-V; Bioniche Animal Health, Belleville ON, Canada). The total dose of FSH was distributed equally over 8 (Control) or 14 (Long) IM injections at 12-h intervals. Prostaglandin F2α was administered twice (at 12-h intervals) on Day 2 (Control) or Day 5 (Long) and PRID were removed 12 h after the last PGF. Both groups received 25 mg of porcine LH (pLH) IM (Lutropin-V; Bioniche Animal Health) 24 h after PRID removal and AI with frozen–thawed semen of proven fertility was done 12 and 24 h later. A third AI was done 12 h later in cows with 2 or more follicles ≥9 mm (12/12 and 9/12 in Control and Long groups, respectively; P = 0.22). All animals were subjected to transrectal ultrasonography every other day and at the time of ova or embryo collection. Ova or embryos were collected nonsurgically 7 days after the pLH injection and evaluated following IETS guidelines. Embryos were defined as transferable (Grades 1, 2 and 3) and freezable (Grades 1 and 2). Procedure Genmod was used to compare variables between treatments and results are presented as means ± SEM (Table 1). There was no significant difference in the total numbers of ova/embryos recovered, but there were more ovulations, corpora lutea (CL), fertilized ova and transferable and freezable embryos in the Long group (P < 0.05). Collection efficiency (number of ova/embryos over the number of CL) was lower in the Long group (P < 0.05). In summary, lengthening of the superstimulatory treatment resulted in an increased number of ovulations without a decrease in oocyte or embryo competence. Data suggest that the traditional 4-day superstimulatory treatment protocol provides inadequate time to maximize ovulatory response. Table 1.Superovulatory response (mean ± SEM) of cows subjected to a conventional 4-day (Control) or a 7-day (Long) treatment protocol

439 OVARY ULTRASOUND EVALUATION OF Santa Inês EWES SUBMITTED TO SHORT DURATION PROTOCOLS WITH PROGESTOGENS ASSOCIATED WITH LECIRELIN AND/OR ESTRADIOL BENZOATE

2010 ◽  
Vol 22 (1) ◽  
pp. 376
Author(s):  
C. E. A. Biscarde ◽  
S. D. Bicudo ◽  
L. C. O. Magalhães ◽  
L. F. Crocomo ◽  
R. F. Bittencourt ◽  
...  
Keyword(s):  
Short Duration ◽  
Transrectal Ultrasound ◽  
Estradiol Benzoate ◽  
Estrus Cycle ◽  
Plasma Progesterone ◽  
Ultrasound Evaluation ◽  
Ovulatory Follicle ◽  
Significant Difference ◽  
Santa Inês ◽  
Average Size

In order to enhance the use of biotechnology in females it is necessary to have good knowledge of the ovarian events, and thus enable the manipulation of the estrus cycle to concentrate ovulations in an established period of time. In this experiment, we evaluated the ovary response and corpus luteum (CL) parameters in a short-duration protocol associated or not to lecirelin (L)(GnRH) and/or estradiol benzoate (EB). Twenty-four cyclic Santa InÊs ewes were divided into 4 groups: Control animals (G1; n = 6) received 45 μg of d-cloprostenol (Prolise®, Tecnopec, Sao Paulo, Brazil) on Day 0. Intravaginal sponges containing medroxyprogesterone acetate (MAP60®, Tecnopec) were inserted on Day 3. Then on Day 7 sponges were removed and 400 IU of eCG and 45 μg of d-cloprostenol were administered. G2 (n = 6) animals underwent the same protocol as G1 but with administration of 1 mg of EB on Day 1; G3 (n = 6) animals underwent the same protocol as G1 but with administration of 25 μg of L (Gestran plus®, Tecnopec) 30 h after sponge withdrawal; G4 (n = 6) animals underwent the same protocol as G1 but with administration of 1 mg of EB on Day 1 and 25 μg of L 30 h after sponge withdrawal. Ewe ovaries were assessed via transrectal ultrasound (US), and CL were measured 4 days after ovulation. On the same day blood samples were taken to measure plasma progesterone levels via radioimmunoassay. Twenty-three out of 24 animals ovulated. One ewe from G4 that did not ovulate was taken out of the experiment. The average size of the largest pre-ovulatory follicle was 7.3 mm (G1), 7.0 mm (G2), 6.8 mm (G3), and 7.4 mm (G4) (P > 0.05). There were 1.8, 1.2, 2.0, and 1.4 ovulated follicles per animal in G1, G2, G3, and G4, respectively. The results for estrus start and duration after sponge withdrawal were G1: 32/38.6; G2: 37/69.3; G3: 29.3/37.3; G4: 44/68 h, respectively. Ovulation time span was G1: 65.3; G2: 88; G3: 53.3; G4: 82.4 h after sponge withdrawal. There was a high correlation of ovary mensuration and progesterone level (r = 0.64; P < 0.0001) on the fourth day after ovulation. Progesterone levels after ovulation were 2.37 ng mL-1 (G1); 1.5 ng mL-1 (G2); 3.22 ng mL-1 (G3) and 1.99 ng mL-1 (G4), being higher in G3 than in G2 (P < 0.05). It was seen that the EB is prejudicial to the CL function. Despite the fact that there was no significant difference of progesterone levels within G1, G3, and G4, animals in G3 displayed higher levels of progesterone; hence, there is a need for further studies using a larger number of animals and fertility test. CNPq e Tecnopec.

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