225 Evaluation of superovulatory response in Brazilian native sheep by B-mode ultrasonography

2020 ◽  
Vol 32 (2) ◽  
pp. 240
Author(s):  
G. B. Vergani ◽  
M. S. D. Lima ◽  
K. M. Silva ◽  
A. W. U. Monteiro ◽  
A. F. Ramos ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Pearson Correlation ◽  
Animal Health ◽  
Device Removal ◽  
Embryo Recovery ◽  
Flunixin Meglumine ◽  
Ultrasound Evaluation ◽  
Release Device ◽  
Linear Probe ◽  
Santa Inês

The objective of this study was to evaluate the correlation between the number of corpora lutea (CL), as determined by B-mode ultrasonography, and the total number of structures recovery by non-surgical embryo recovery (NSER) from superovulated ewes. Somali (n=18), Santa Inês (n=18), and Morada Nova (n=16) ewes received an intravaginal device with progesterone (0.33g, controlled internal drug release device, CIDR, Zoetis), which was maintained for 9 days. The superovulatory protocol started 60h before device removal, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) of p-FSH (IM 133mg, Folltropin V, Vetoquinol) injected every 12h. D-Cloprostenol (IM 37.5μg, Prolise, Agener Union) was injected 12h before and at the moment of device removal. Ewes in oestrus were mated by fertile male rams three times after the onset of oestrus with a 12-h interval. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was injected IM on Days 12, 13, and 15. One day before NSER, B-mode ultrasound evaluations were performed using portable equipment (Z5 Vet, Mindray) with a stiffened multifrequency linear probe to evaluate the number of structures present in the ovaries. Embryo recovery was performed 7 days after progesterone CIDR removal (Day 16). The number of CL and the number of recovered structures were determined and their association evaluated using the Pearson correlation test (P<0.05). Only 17 Somali, 16 Santa Inês, and 15 Morada Nova ewes were submitted to NSER; the other 4 ewes could not be flushed because of low cervical dilation or cervical puncture. After B-mode ultrasound evaluation, 168, 217, and 131 CL were observed, with an average of 9.9 (range: 1 to 12), 13.5 (range: 2 to 47), and 8.7 (range: 3 to 18) CL per donor for Somali, Santa Inês, and Morada Nova ewes, respectively. The recovery rates (i.e. structures recovered by the number of CL counted) were 60.1% (101/168), 96.3% (209/217), and 103.5% (135/131) for the 3 breeds, respectively. There was a positive correlation (r=0.69; P<0.01) between the number of recovered structures and number of CL. Those findings confirm results from other studies, in which the recovery rate varied from 35 to 91%. In some cases, recovery rate can be overestimated because of the difficulty in quantifying CL. In conclusion, B-mode ultrasonography can be used to estimate superovulatory response in ewes and thus as a criterion to decide whether a donor will undergo embryo flushing. Financial support for this study was provided by Embrapa (02.13.06.026.00.04) and Fapemig (CVZ-PPM 00201-17).

227 Is the antral follicle count on a random day of the oestrous cycle correlated with superovulatory responses in Santa Inês ewes?

2020 ◽  
Vol 32 (2) ◽  
pp. 241
Author(s):  
M. Pupin ◽  
G. Vergani ◽  
M. Lima ◽  
K. Silva ◽  
A. Monteiro ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Animal Health ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Flunixin Meglumine ◽  
Santa Inês ◽  
Synchronization Protocol

Antral follicle count (AFC) performed after an oestrus synchronization protocol has been studied as a tool to select ewes with high potential for invivo embryo production (Pinto et al. 2018 Theriogenology 113, 146-152). However, it would be interesting to know whether AFC assessed on a random day of the oestrous cycle correlates with the superovulatory response. The present study was conducted to evaluate the correlation between AFC at the beginning of progesterone (P4)-based oestrus synchronization protocol used as basis of superovulatory treatment and the number of corpora lutea (CL) 12h before recovery of embryos in Santa Inês ewes. The study was conducted during September and October in northeast Brazil (03°40′26″S and 40°14′20″W) using 8 adult Santa Inês ewes. On a random day of oestrous cycle (Day 0) all ewes received an intravaginal device (CIDR) of progesterone (0.3g, Eazi-breed, Zoetis), which remained for 9 days. On Day 7, the pFSH (133mg, Folltropin V, Vetoquinol) treatment began, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) injected IM at 12-h intervals. On Day 9, 2 equal doses of D-cloprostenol were injected at a 12-h interval (37.5µg, Prolise, Agener União). All ewes showed oestrus and were mated by fertile rams. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was administered IM on Days 12, 13, and 15. On Day 16, non-surgical embryo recovery (NSER) was performed after cervical dilation using D-cloprostenol and oestradiol benzoate at 16h and oxytocin 20min before. Transrectal B-mode ultrasound evaluations (Z5 Vet, Mindray), frequency 7.5MHz, were performed on Day 0 and 7 and 12h before NSER to evaluate the ovarian population present. Pearson correlation analysis (P<0.05) was performed using Bioestat 5.3 software. The number of AFC per ewe at the beginning of the protocol and on Day 7 were 9.9±2.7 and 11±3.2, respectively. The numbers of CL, recovered embryos, and viable embryos were 14.0±3.5, 8.2±10.9, and 6.0±11.0, respectively. There was no correlation of AFC on a random day of oestrous cycle with the number of AFC on Day 7 (P=0.42), number of corpora lutea (P=0.44), number of recovered embryos (P=0.18), or number of viable embryos (P=0.11) in superovulated ewes. In conclusion, we did not find significant correlations between AFC on a random day of oestrous cycle and the superovulatory/embryos response in Santa Inês ewes. Financial support for this study was provided by Embrapa (02.13.06.026.00.02 and 02.13.06.026.00.04) and FAPEMIG (PPM 00201-17).

108 COMPARATIVE EFFICIENCY OF GONADOTROPIN-RELEASING HORMONE AND LUTEINIZING HORMONE IN THE INDUCTION OF OVULATION IN SUPEROVULATED ALPACAS

2014 ◽  
Vol 26 (1) ◽  
pp. 168
Author(s):  
H. W. Vivanco-Mackie ◽  
M. D. Ponce Salazar ◽  
M. M. Gonzales ◽  
M. A. Tapia
Keyword(s):  
Gnrh Agonist ◽  
Recovery Rate ◽  
Pearson Correlation ◽  
Animal Health ◽  
Gonadotropin Releasing Hormone ◽  
Corpora Lutea ◽  
Peruvian Andes ◽  
Releasing Hormone ◽  
Induction Of Ovulation ◽  
Embryo Recovery

Alpacas are induced ovulators, responding to copulation and (or) exogenous application of ovulation-inducing hormones. The objective of this study was to determine the efficiency of the injection of a gonadotropin-releasing hormone (GnRH) agonist versus LH in the induction of ovulation and the presence and size of non-ovulated follicles at the time of embryo collection and its relationship to the yield of transferable embryos in superovulated alpacas. Twenty-one adult (3 to 7 years old) female alpacas under extensive grazing at 4300 m elevation in the Peruvian Andes that had been synchronized and treated for superovulation were induced to ovulate 6 days after the application of the superovulatory hormone (1000 IU of eCG, Folligon®, Intervet International BV, Boxmeer, the Netherlands) by mating with fertile males and injection immediately after copulation of either an IM dose of 0.0084 mg of buserelin acetate (Buserelina Zoovet®, Laboratorio Zoovet, Santa Fe, Argentina) to 10 alpacas (T1) or an IM dose of 5-mg Armour standard of LH (Lutropin®, Bioniche Animal Health, Belleville, ON, Canada) to 11 alpacas (T2). All alpacas had a second mating 12 h after the first mating and were subjected to ovarian inspection by ultrasonography and previous embryo collection by nonsurgical transcervical embryo flushing 6.5 days after the first mating. On average, the embryo recovery rate for T1 was 34.6% and there were 7.8 ± 3.99 corpora lutea (CL), 2.7 ± 4.08 collected embryos, and 3.6 ± 2.95 total, 0.5 ± 0.85 small (<6 mm), 1.8 ± 1.99 medium (≥6 but ≤14 mm), and 1.3 ± 2.11 large (≥15 mm) non-ovulated follicles. For T2, the embryo recovery rate was 59.4% and there were 6.73 ± 1.49 CL, 4.0 ± 2.57 collected embryos, and 0.64 ± 0.81 total, 0.0 ± 0.0 small, 0.36 ± 0.67 medium, and 0.27 ± 0.47 large non-ovulated follicles. The differences between treatments were nonsignificant (P > 0.05) for all the parameters; however, there was a clear tendency for a better recovery rate, more embryos collected, and lower number of non-ovulated follicles in T2. The Pearson correlation coefficient between the presence of large follicles in the ovaries at the time of embryo collection and the total number of embryos collected was negative (r = –0.26) and highly significant (P ≤ 0.001). The use of LH for ovulation induction of superovulated alpacas seems to be more advisable than the use of GnRH agonist; further comparisons with larger number of observations per treatment are recommended. This study was financed by the Peruvian Fund for Innovation, Science and Technology (FINCYT).

Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH

2003 ◽  
Vol 49 (1) ◽  
pp. 19-23 ◽  
Author(s):  
M.F. Cordeiro ◽  
J.B. Lima-Verde ◽  
E.S. Lopes-Júnior ◽  
D.I.A. Teixeira ◽  
L.N. Farias ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Embryo Recovery ◽  
Santa Inês

226 Effect of the presence or absence of corpus luteum in females with small antral follicles at the beginning of a progesterone-based oestrus synchronization protocol on superovulatory response and embryo yields in sheep

2020 ◽  
Vol 32 (2) ◽  
pp. 241
Author(s):  
M. S. D. Lima ◽  
G. B. Vergani ◽  
K. M. Silva ◽  
A. W. U. Monteiro ◽  
L. M. C. Pereira ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Animal Health ◽  
Cervical Dilation ◽  
Antral Follicles ◽  
Embryo Recovery ◽  
Multiple Ovulation ◽  
Flunixin Meglumine ◽  
Oestradiol Benzoate ◽  
Intravaginal Device ◽  
Synchronization Protocol

The high variability of responses to multiple ovulation and embryo transfer (MOET) programs is related to several factors, including the ovarian population present at the beginning of hormonal protocols. Thus, our objective was to evaluate the effect of the presence or absence of corpus luteum (CL) in females presenting small antral follicles (SF) at the beginning of a progesterone (P4)-based oestrus synchronization protocol on superovulatory response and embryo yields. The study was conducted during September and October in northeast Brazil (03°40′26″S and 40°14′20″W) using 15 adult Santa Inês ewes, in which the ovarian population present were SF (≤3.75mm, n=5) or SF plus CL (SF+CL, n=10) at the beginning of P4-based oestrus synchronization protocol. The females received a new or reused intravaginal device of progesterone (CIDR Eazi-breed, Zoetis) on Day 0, which remained for 9 days. On Day 7, the pFSH (133mg, Folltropin V, Vetoquinol) treatment was begun, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) injected IM at 12-h intervals. On Day 9, two equal doses of D-cloprostenol were injected at a 12-h interval (37.5μg, Prolise, Agener União). Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was administered IM on Days 13 to 15. All ewes showed oestrus and were mated with fertile rams. On Day 16 non-surgical embryo recovery (NSER) was performed after cervical dilation using D-cloprostenol and oestradiol benzoate at 16h and ocytocin at 20min before. Transrectal B-mode ultrasound evaluations (Z5 Vet, Mindray) were performed on Day 0 and just before NSER to evaluate the ovarian population present. Data are showed in mean±s.d. and compared by t-test for independent samples (P&lt;0.05). The number of CL present on NSER day (21.5±4.4 and 4.0±8.5) and the number of viable embryos (11.1±14.9 and 4.0±8.5) differed (P&lt;0.05) between SF+CL ewes and SF ewes, respectively. However, the number of luteinizing unovulated follicles present on NSER day (4.5±2.5 and 12.4±7.8) and the number of recovered structures (i.e. embryos plus unfertilized eggs; 14.2±15.6 and 4.2±8.2) did not differ (P&gt;0.05) between SF+CL ewes and SF ewes, respectively. In conclusion, the presence of a CL at the beginning of the oestrous synchronization protocol used for superovulation treatment affects responses to the MOET program. Financial support for this study was provided by Embrapa (02.13.06.026.00.02 and 02.13.06.026.00.04) and FAPEMIG (PPM 00201-17).

Successive in vivo embryo production in Santa Inês sheep

2020 ◽  
Vol 60 (4) ◽  
pp. 497 ◽  
Author(s):  
Pedro Henrique Nicolau Pinto ◽  
Mario Felipe Alvarez Balaro ◽  
Helena Fabiana Reis de Almeida Saraiva ◽  
Viviane Lopes Brair ◽  
Vivian Angélico Pereira Alfradique ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Doppler Ultrasonography ◽  
Corpora Lutea ◽  
Colour Doppler ◽  
Embryo Production ◽  
Previous Response ◽  
Embryo Recovery ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P &lt; 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P &lt; 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P &lt; 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, &gt;10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

Effect of season and frequency of embryo collections on superovulatory response and embryo recovery in Santa Inês hair sheep

2021 ◽  
pp. 106441
Author(s):  
Monique de Albuquerque Lagares ◽  
Fabiana Cristina Varago ◽  
Valéria Spyridion Moustacas ◽  
Valentim Arabicano Gheller ◽  
Rafael Romero Nicolino ◽  
...  
Keyword(s):  
Hair Sheep ◽  
Embryo Recovery ◽  
Santa Inês

Leptospirosis and embryo recovery rate in mares: Table 1

2012 ◽  
Vol 170 (2) ◽  
pp. 60-60 ◽  
Author(s):  
A. Pinna ◽  
G. Martins ◽  
W. Lilenbaum
Keyword(s):  
Recovery Rate ◽  
Embryo Recovery

Embryo Recovery Rate in a Mare Affected by Cushing's Syndrome

2003 ◽  
Vol 27 ◽  
pp. 619-621 ◽  
Author(s):  
D. Panzani ◽  
I. Vannozzi ◽  
M. Sgorbini ◽  
M. Corazza ◽  
A. Rota ◽  
...  
Keyword(s):  
Cushing’S Syndrome ◽  
Recovery Rate ◽  
Cushing's Syndrome ◽  
Embryo Recovery

scholarly journals 171IMPROVEMENT IN EMBRYO RECOVERY USING UTERINE DOUBLE FLUSHING

2004 ◽  
Vol 16 (2) ◽  
pp. 207 ◽  
Author(s):  
A. Castro Neto ◽  
B.V. Sanches ◽  
S.H.V. Perri ◽  
M.M. Seneda ◽  
J.F. Garcia
Keyword(s):  
Recovery Rate ◽  
Foley Catheter ◽  
Test Group ◽  
Control Group ◽  
Recovery Rates ◽  
Mean Values ◽  
Embryo Recovery ◽  
Group A ◽  
Uterine Flushing ◽  
The Mean

The aim of the present study was to evaluate the effects of uterine double flushing on embryo recovery rates (total structures and viable embryos), after a resting period with the uterus filled with PBS. There were 210 embryo recovery procedures conducted using the uterine double flushing method, and the results were compaired with 432 conventional single flushing procedures. All procedures were conducted with Limousin (n=403) and Guzerá (n=239) cows, following the same superovulation protocol. Cyclic donors received a progestagen implant (CIDR) and 1mL of oestradiol benzoate (Estrogin®) at Day 0. Between Day 5 and Day 9 animals received doses ranging from 200 to 300UI (zebu cows) and 300 to 500UI (taurine cows) of FSH (Pluset® – Serono) in decreasing doses. Between Day 6 and Day 8 PGF2a (Ciosin®) was administered, followed by withdrawal of the progestagen after 24h. Artificial insemination was performed between 14 and 26h after the beginning of treatment. For the double flushing procedure, after the first uterine flushing of both horns with 1L DPBS (Nutricell), a Foley catheter was positioned in the uterine body in order to fill the uterus with the same solution (80 to 150mL). After this procedure the catheter was closed with a disposable 5-mL syringe gasket, and the animals were allowed to rest in the surroundings of the work place for 30min. After this period, a second flush was performed in order to recover the remaning liquid used during manipulation of the uterus. Animals from the control group (group A) were subjected to a single uterine flushing procedure. From 210 double flushing procedures (group B – test), 1409 viable embryos were produced, classified as grades I, II, III and IV (IETS), (average of 6.7 embryos per procedure), whereas, in the 432 single flushing procedure (group A – control), 1993 embryos were produced (average of 4.6). Statistical analysis showed the increase of viable embryo recovery rate. When consecutive double flushing was performed, the average of recovered embryos increased from 8.3 to 12.7 (P&lt;0.05) in Limousin cows, and from 7.9 to 11.5 (P&lt;0.05) in Guzerá cows. Comparing recovery after single flushing with that after double flushing, the mean number of viable embryos increased from 4.7 to 6.9 (P&lt;0.05) in Limousin cows and from 4.5 to 6.4 (P&lt;0.05) in Guzerá cows. In order to assure the nonexistence of negligence effects or operator influence on results, the mean values of total embryo recovery rate after single flushing (control group) was compared to the mean values of the same rate after the first uterine flushing on test group. Results indicated no difference between recovery rates. The present work showed the viability of using the uterine double flushing procedure for improvement of embryo recovery rates in cattle.

scholarly journals Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 145-151
Author(s):  
J.P. Ramon-Ugalde ◽  
J. Folch ◽  
M.J. Cocero ◽  
R.E. Piña-Aguilar ◽  
J.L. Alabart
Keyword(s):  
Recovery Rate ◽  
Uterine Horn ◽  
Conception Rate ◽  
The Other ◽  
Embryo Recovery ◽  
Intramuscular Injections ◽  
Other Hand

The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and seventy ewes of Aragonesa breed were used as embryo donors and receptors, respectively. The donors were distributed in 3 replications. The oestrus was synchronized with the insertion of FGA intravaginal sponges (30 mg) for 13 days. Six intramuscular injections of 18 mg pFSH were applied in decreasing doses at 12 hour intervals starting 48 hours after sponge withdrawal. The recovery rate (RR) (83.2 vs. 75.8%), the viability rate (VR) (73.5 vs. 47.2%) and the number of viable embryos (VE) per donor ewe (5.9 ± 0.79 vs. 3.0 ± 0.37) were higher (<I>P</I> < 0.01) in ewes whose perfusion was done directly in oviducts. The interval from sponge withdrawal to oestrus had no influence on RR in any groups; however it had an effect on the VR in 3.5 and 7 days old embryos. Embryos from ewes showing the oestrus within 20 hours after sponge withdrawal had a higher viability. On the other hand, using morphologic criteria, 26.5% and 52.7% of 3.5 and 7 days old embryos, respectively, were discarded before transfer. The percentage of ewes in each group that became pregnant was similar in both groups. We conclude that in superovulated ewes the embryo recovery rate, viability and number of viable embryos are higher in embryos recovered from the oviduct compared to those recovered from the uterus, without decreasing the conception rate and the viability rate is higher in ewes that show the oestrus 20 hours before sponge withdrawal.

Sign in / Sign up

Export Citation Format

Share Document