A phylogeny inferred from large-subunit (26S) ribosomal DNA sequences suggests that the family Dasypogonaceae is closely aligned with the Restionaceae allies

2002 ◽  
Vol 15 (6) ◽  
pp. 749 ◽  
Author(s):  
Ray Neyland
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Monophyletic Group ◽  
26S Rdna ◽  
Rdna Sequences ◽  
Australian Family ◽  
The Family

The Dasypogonaceae is a small Australian family composed of four genera. Previous systematic studies have failed to place the Dasypogonaceae with confidence. The present phylogenetic analysis, inferred from large-subunit (26S) rDNA sequences, strongly suggests that the Dasypogonaceae form a monophyletic group with the taxa referred to as the Restionaceae allies (i.e. Anarthriaceae, Centrolepidaceae, Ecdeiocoleaceae).

scholarly journals Sequences of psychrophilic fungi amplified from glacier-preserved ascolichens

2000 ◽  
Vol 78 (11) ◽  
pp. 1450-1459 ◽  
Author(s):  
Paula T DePriest ◽  
Natalia V Ivanova ◽  
Dianne Fahselt ◽  
Vagn Alstrup ◽  
Andrea Gargas
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Ice Core ◽  
Small Subunit ◽  
Rdna Sequences ◽  
Three Samples ◽  
Tyrolean Iceman ◽  
Small Subunit Ribosomal Dna

Ribosomal DNA sequences were amplified from subfossils of the ascolichen Umbilicaria cylindrica (L.) Delise ex Duby collected at the ablating edges of Greenland glaciers. Surprisingly, phylogenetic analysis indicated that the amplified rDNA sequences were not closely related to those of the lichen-forming fungus but rather represented two groups of psychrophilic basidiomycetes (orders Cystofilobasidiales and Sporidiales) and one group of ascomycetes (order Leotiales). Two of these groups, the Sporidiales and the Leotiales, include other fungi previously detected in DNA extracted from the grass clothing of the Tyrolean Iceman desiccated and frozen for over 3000 years and also in 2000- and 4000-year-old ice core samples from northern Greenland. Large subunit ribosomal DNA sequences representing the group Cystofilobasidiales were nearly identical to those of the basidioyeast saprobe Mrakia frigida. The adjacent internal transcribed spacer sequence was more than 98% similar to those from three samples of U. cylindrica from different sites that had been subjected to ice burial for various lengths of time, suggesting they also were Mrakia sequences. Although ancient contamination of multiple U. cylindrica specimens with fungi such as Mrakia cannot be ruled out, it is more probable that saprobic colonization of the subfossil tissues by psychrophilic fungi proceeded during recent ice melt.Key words: ancient DNA, small subunit ribosomal DNA, 18S ribosomal DNA, phylogenetic analysis, psychrophilic fungi, lichen-forming fungi.

On the subdivision of Ceratocystis s.l., based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (1) ◽  
pp. 52-63 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Key Words ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Cell Walls ◽  
Large Subunit ◽  
Rdna Sequences

Analyses of partial rDNA sequences from both the small and large subunit genes of species of Ceratocystis s.l. support the contention that species that lack Chalara anamorphs, are resistant to cycloheximide, and have rhamnose in their cell walls should be assigned to Ophiostoma, whereas only species with Chalara anamorphs should be accommodated in Ceratocystis s.s. The data also show that Ceratocystiopsis is polyphyletic, and Sphaeronaemella fimicola appears to have little relation to either Ceratocystis or Ophiostoma. Key words: Ceratocystis, Ophiostoma, phylogeny, partial rDNA sequences.

Sequence characterization and phylogenetic analysis of the 5S ribosomal DNA in species of the family Batrachoididae

Genome ◽  
2010 ◽  
Vol 53 (9) ◽  
pp. 723-730 ◽  
Author(s):  
María Úbeda-Manzanaro ◽  
Manuel Alejandro Merlo ◽  
José Luis Palazón ◽  
Carmen Sarasquete ◽  
Laureana Rebordinos
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
5S Rdna ◽  
Purifying Selection ◽  
The Other ◽  
Fish Family ◽  
Sequence Characterization ◽  
Rdna Sequences ◽  
The Family ◽  
Clear Differentiation

5S ribosomal DNA (rDNA) sequences were analyzed in four species belonging to different genera of the fish family Batrachoididae. Several 5S rDNA variants differing in their non-transcribed spacers (NTSs) were found and were grouped into two main types. Two species showed both types of 5S rDNA, whereas the other two species showed only one type. One type of NTS of Amphichthys cryptocentrus showed a high polymorphism due to several deletions and insertions, and phylogenetic analysis showed a between-species clustering of this type of NTS in Amphichthys cryptocentrus. These results suggest a clear differentiation in the model of 5S rDNA evolution of these four species of Batrachoididae, which appear to have been subject to processes of concerted evolution and birth-and-death evolution with purifying selection.

Phylogeny of dracunculoid nematodes (Chromadorea: Rhabditida: Spirurina: Dracunculoidea) from some Eurasian freshwater fishes

Zootaxa ◽  
2020 ◽  
Vol 4858 (4) ◽  
pp. 521-541
Author(s):  
SERGEY G. SOKOLOV ◽  
ALEXANDER P. KALMYKOV ◽  
SVETLANA V. MALYSHEVA
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Relationship ◽  
Ribosomal Dna ◽  
Phylogenetic Relationships ◽  
Type Species ◽  
Freshwater Fishes ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Rdna Sequences ◽  
The Family

Sets of small ribosomal DNA (SSU rDNA) and large ribosomal DNA (LSU rDNA) sequences were obtained for Philometroides moraveci Vismanis & Yunchis, 1994, Philometra kotlani (Molnár, 1969), Philometra rischta Skrjabin, 1923, Philometra cf. obturans (Prenant, 1886) (Philometridae), Sinoichthyonema amuri (Garkavi, 1972), Agrachanus scardinii (Molnár, 1966), Kalmanmolnaria intestinalis (Dogiel & Bychowsky, 1934) and Skrjabillanus tincae Shigin & Shigina, 1958 (Skrjabillanidae). Phylogenetic analysis of SSU rDNA data shows that dracunculoid nematodes are divided into two well-supported clades designated as Clade I and Clade II, respectively. Clade I includes the type species of the genus Philonema Kuitunen-Ekbaum, 1933, some species from the family Daniconematidae Moravec & Køie, 1987 and two subfamilies of skrjabillanids, Skrjabillaninae Shigin & Shigina, 1958 and Esocineminae Moravec, 2006. Clade II unites species from the families Dracunculidae Stiles, 1907, Micropleuridae Baylis & Daubney, 1926 and Philometridae Baylis & Daubney, 1926. Within the Philometridae, there are several well-supported groups of species, one of which unites freshwater Philometra spp. from the Palearctic cyprinids, identified as P. kotlani, P rischta, P. ovata (Zeder, 1803) and P. cyprinirutili (Creplin, 1825). However, the phylogenetic relationships of most philometrids are unresolved. An analysis of partial SSU and LSU rDNA sequences indicates that there is no direct phylogenetic relationship between Agrachanus Tikhomirova, 1971 (type species Skrjabillanus scardinii Molnár, 1966) and Skrjabillanus Shigin & Shigina, 1958 (type species Sk. tincae), which means that the genus Agrachanus can be resurrected. Our study confirms that Philonematinae Ivashkin, Sobolev & Khromova, 1971 should be elevated to the family rank. We formally establish the family Philonematidae Ivashkin, Sobolev & Khromova, 1971 stat. nov. We also suggest combining the superfamilies Dracunculoidea Stiles, 1907 and Camallanoidea Railliet & Henry, 1915 into the infraorder Camallanomorpha Roberts, Janovy & Nadler, 2013. 

A phylogeny inferred from large-subunit (26S) ribosomal DNA sequences suggests that Burmanniales are polyphyletic

2002 ◽  
Vol 15 (1) ◽  
pp. 19 ◽  
Author(s):  
Ray Neyland
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Monophyletic Group

According to several authors, the Burmanniales are an order that includes the families Burmanniaceae, Thismiaceae and Corsiaceae. At present, there is no consensus concerning the circumscription of Burmanniales. All members of Thismiaceae and Corsiaceae are mycoheterotrophic; Burmanniaceae are composed of both mycoheterotrophic and autotrophic species. Other than mycoheterotrophy, there are few characters that unite members of the order. Because mycoheterotrophy has resulted in extreme vegetative reduction that may have contributed to homoplasy in the form of character convergence, the systematics of Burmanniales is problematic. A phylogeny inferred from large-subunit (26S) ribosome-DNA sequences suggests that the order Burmanniales is polyphyletic. Specifically, the Burmanniaceae appears as strongly supported monophyletic group with Thismia as sister. Corsia is distinct and may be associated with the Liliales.

Phylogeny of the rust fungi: an approach using nuclear large subunit ribosomal DNA sequences

2003 ◽  
Vol 81 (1) ◽  
pp. 12-23 ◽  
Author(s):  
Wolfgang Maier ◽  
Dominik Begerow ◽  
Michael Weiß ◽  
Franz Oberwinkler
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Rust Fungi ◽  
Monophyletic Group ◽  
Neighbor Joining ◽  
Natural Group ◽  
Outgroup Species ◽  
Large Subunit Rdna

Sequence data from nuclear large subunit ribosomal DNA was used to infer phylogenetic relationships of selected genera of the Uredinales. We investigated 52 rust fungi representing nine families and three outgroup species. Neighbor joining analysis and a Bayesian method of phylogenetic inference using Monte Carlo Markov chains confirm the rust fungi as a natural group and indicate that Puccinia, Uromyces, Endophyllum, and Cumminsiella have a common origin. The autoecious Rosaceae-rusts Phragmidium, Kuehneola, Triphragmium, and Trachyspora are a monophyletic group. The gasteroid genus Ochropsora is closely related to Tranzschelia. While the Pucciniastreae sensu Dietel (1938) is recognized as a monophyletic group in neighbor joining analysis, the Pucciniaceae s.l. (Dietel 1928) is supported by Bayesian analysis. The following genera appear to be monophyletic: Chrysomyxa, Coleosporium, Cronartium, Gymnosporangium, Melampsora, Phragmidium, and Tranzschelia, whereas the genera Puccinia, Pucciniastrum, Thekopsora, and Uromyces are not.Key words: molecular phylogeny, systematics, nuclear large subunit rDNA, Basidiomycota, Urediniomycetes, Uredinales.

scholarly journals Discovery of Novel Backusella (Backusellaceae, Mucorales) Isolated from Invertebrates and Toads in Cheongyang, Korea

2021 ◽  
Vol 7 (7) ◽  
pp. 513
Author(s):  
Thuong T.T. Nguyen ◽  
Kerstin Voigt ◽  
André Luiz Cabral Monteiro de Azevedo Santiago ◽  
Paul M. Kirk ◽  
Hyang-Burm Lee
Keyword(s):  
Ribosomal Dna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characters ◽  
Fungal Species ◽  
Lsu Rdna ◽  
Rdna Sequences ◽  
The Family ◽  
Internal Transcribed Spacer Sequences

Three novel fungal species, Backusella chlamydospora sp. nov., B. koreana sp. nov., and B. thermophila sp. nov., as well as two new records, B. oblongielliptica and B. oblongispora, were found in Cheongyang, Korea, during an investigation of fungal species from invertebrates and toads. All species are described here using morphological characters and sequence data from internal transcribed spacer sequences of ribosomal DNA and large subunit of the ribosomal DNA. Backusella chlamydospora is different from other Backusella species by producing chlamydospores. Backusella koreana can be distinguished from other Backusella species by producing abundant yeast-like cells. Backusella thermophila is characterized by a variable (subglobose to oblong, applanate to oval, conical and ellipsoidal to pyriform) columellae and grows well at 37 °C. Multigene phylogenetic analyses of the combined ITS and LSU rDNA sequences data generated from maximum likelihood and MrBayes analyses indicate that B. chlamydospora, B. koreana, and B. thermophila form distinct lineages in the family Backusellaceae. Detailed descriptions, illustrations, phylogenetic tree, and taxonomic key to the Backusella species present in Korea are provided.

Phylogenetic relationships in Neonectria species having Cylindrocarpon anamorphs inferred from mitochondrial ribosomal DNA sequences

2001 ◽  
Vol 79 (3) ◽  
pp. 334-340 ◽  
Author(s):  
Feky R Mantiri ◽  
Gary J Samuels ◽  
James E Rahe ◽  
Barry M Honda
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequence ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Sequence Divergence ◽  
Small Subunit ◽  
New Combinations ◽  
Rdna Sequences ◽  
Informal Groups

Mitochondrial small subunit (mtSSU) rDNA sequences elucidated phylogenetic relationships in Neonectria Wollenw. (anamorphs = Cylindrocarpon Wollenw.; Ascomycetes, Hypocreales). Twelve isolates representing seven species in five taxonomically informal groups of Neonectria were subjected to phylogenetic analysis. Fusarium inflexum R. Schneid. (teleomorph: Gibberella) and Nectria cinnabarina (Fr.) Fr. (= Nectria s.str.) were outgroups. All of the Neonectria species formed a strongly supported clade with respect to the outgroups, indicating a single ascomycete genus for the holomorphs of Cylindrocarpon. Within the Neonectria clade there were three well-supported subclades that only partially corresponded to phenotype-defined groups. DNA sequence divergence among the twelve Neonectria isolates, 2.3-7.4%, was sufficient to resolve them. The results suggest that the mtSSU rDNA region is appropriate for phylogenetic analysis of Neonectria and Cylindrocarpon. The following new combinations are proposed: Neonectria coronata, Neonectria discophora, Neonectria neomacrospora, Neonectria radicicola, Neonectria rugulosa, Neonectria veuillotiana.Key words: Ascomycetes, Hypocreales, Nectria, systematics, tree pathogens.

Phylogenetic analysis of the superfamily Hemiuroidea (Platyhelminthes, Neodermata:Trematoda) based on partial 28S rDNA sequences

Parasitology ◽  
2018 ◽  
Vol 146 (5) ◽  
pp. 596-603 ◽  
Author(s):  
Sergey G. Sokolov ◽  
Dmitry M. Atopkin ◽  
Misako Urabe ◽  
Ilya I. Gordeev
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Relationship ◽  
Phylogenetic Relationships ◽  
Sister Group ◽  
28S Rdna ◽  
Monophyletic Group ◽  
Rdna Sequences ◽  
The Family ◽  
Statistical Support ◽  
28S Rdna Sequences

AbstractIn the present paper, the phylogenetic relationships between genera, subfamilies and families of the Hemiuroidea are explored. Twelve new sequences of 28 rDNA and data taken from GenBank (NSBI) on 43 species affiliated to 34 genera were included in the analysis. Most of the hemiuroidean trematodes form two highly supported clades (A and B), which are sister groups to each other.Hemipera manterijoined withGonocercaspp. with moderate statistical support. This clade is basal relative to the clades A and B. Сlade A is polytomic and contains representatives of the families Accacoeliidae, Syncoeliidae, Didymozoidae, Hirudinellidae and Sclerodistomidae, and derogenid subfamilies Derogeninae and Halipeginae. At the same time, the Syncoeliidae, Hirudinellidae and Accacoeliidae form a well-supported monophyletic group. The phylogenetic relationship between Derogeninae and Halipeginae is poorly resolved. Сlade B unites the isoparorchiid, bunocotylid, lecithasterid and hemiurid trematodes. Our data re-establishes the family Bunocotylidae, which consists of two subfamilies, Opisthadeninae and Bunocotylinae, and theMachidatrema chilostoma+Hysterolecithoides frontilatusgroup. The Bunocotylidae is the sister group to the Hemiuridae + Lecithasteridae group and the Isoparorchiidae is a basal relative to the representatives of these three hemiuroid families.

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