scholarly journals Gap junctions on hippocampal mossy fiber axons demonstrated by thin-section electron microscopy and freeze fracture replica immunogold labeling

2007 ◽  
Vol 104 (30) ◽  
pp. 12548-12553 ◽  
Author(s):  
F. Hamzei-Sichani ◽  
N. Kamasawa ◽  
W. G. M. Janssen ◽  
T. Yasumura ◽  
K. G. V. Davidson ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Gap Junctions ◽  
Thin Section ◽  
Mossy Fiber ◽  
Freeze Fracture ◽  
Immunogold Labeling ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

scholarly journals STUDIES OF EXCITABLE MEMBRANES

1974 ◽  
Vol 63 (2) ◽  
pp. 567-586 ◽  
Author(s):  
John E. Rash ◽  
Mark H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Nerve Terminal ◽  
Freeze Fracture ◽  
Staining Procedure ◽  
Fiber Types ◽  
Mammalian Skeletal Muscle ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

The neuromuscular junctions and nonjunctional sarcolemmas of mammalian skeletal muscle fibers were studied by conventional thin-section electron microscopy and freeze-fracture techniques. A modified acetylcholinesterase staining procedure that is compatible with light microscopy, conventional thin-section electron microscopy, and freeze-fracture techniques is described. Freeze-fracture replicas were utilized to visualize the internal macromolecular architecture of the nerve terminal membrane, the chemically excitable neuromuscular junction postsynaptic folds, and the electrically excitable nonjunctional sarcolemma. The nerve terminal membrane is characterized by two parallel rows of 100–110-Å particles which may be associated with synpatic vesicle fusion and release. On the postsynpatic folds, irregular rows of densely packed 110–140-Å particles were observed and evidence is assembled which indicates that these large transmembrane macromolecules may represent the morphological correlate for functional acetylcholine receptor activity in mammalian motor endplates. Differences in the size and distribution of particles in mammalian as compared with amphibian and fish postsynaptic junctional membranes are correlated with current biochemical and electron micrograph autoradiographic data. Orthogonal arrays of 60-Å particles were observed in the split postsynaptic sarcolemmas of many diaphragm myofibers. On the basis of differences in the number and distribution of these "square" arrays within the sarcolemmas, two classes of fibers were identified in the diaphragm. Subsequent confirmation of the fiber types as fast- and slow-twitch fibers (Ellisman et al. 1974. J. Cell Biol. 63[2, Pt. 2]:93 a. [Abstr.]) may indicate a possible role for the square arrays in the electrogenic mechanism. Experiments in progress involving specific labeling techniques are expected to permit positive identification of many of these intriguing transmembrane macromolecules.

The Infection of Cells by Bullet-Shaped Viruses

1969 ◽  
Vol 27 ◽  
pp. 372-373
Author(s):  
Frederick A. Murphy ◽  
Alyne K. Harrison ◽  
Sylvia G. Whitfield
Keyword(s):  
Electron Microscopy ◽  
Physical Properties ◽  
Host Cell ◽  
Thin Section ◽  
Cultured Cells ◽  
Cell Infection ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

scholarly journals STUDIES OF THE ULTRASTRUCTURE AND RIBOSOMAL ARRANGEMENTS OF THE PLEUROPNEUMONIA-LIKE ORGANISM A5969

1965 ◽  
Vol 25 (1) ◽  
pp. 139-150 ◽  
Author(s):  
Jack Maniloff ◽  
Harold J. Morowitz ◽  
Russell J. Barrnett
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Chemical Analysis ◽  
Thin Section ◽  
Differential Centrifugation ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy ◽  
Cellular Organelles

Thin-section electron microscopy, together with isolation of cellular organelles by differential centrifugation and chemical analysis, has been used to investigate the ultrastructure of the avian pleuropneumonia-like organism A5969. Each cell (approximate diameter 5500 A) was surrounded by a 150 A plasma membrane. In the center of the cell was an unbounded area, granular in appearance and containing the cell's DNA. The periphery of the cell contained granules of several different sizes and densities. The most dense particles (150 A) corresponded to the 78S ribosomes. These particles exhibited two predominant arrangements: (a) sometimes they showed cubic packing; (b) most arrays, however, were consistent with cylindrical arrangements of approximately 50 particles. Bundles of up to 18 arrays were observed. Structured blebs have been found protruding from the surface of log phase cells.

Mycoplasma-like organisms as causal agents of potato purple top wilt in Queensland

1985 ◽  
Vol 36 (3) ◽  
pp. 443 ◽  
Author(s):  
RM Harding ◽  
DS Teakle
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Strong Evidence ◽  
Tomato Plant ◽  
Tomato Plants ◽  
Potato Plants ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Potato Purple Top ◽  
Section Electron Microscopy

The eggplant little-leaf agent was graft transmitted to tomato causing big-bud symptoms. Transmission from the big-bud tomato to potato by grafting or the leafhopper Orosius argentatus resulted in the development of purple top wilt symptoms. Thin-section electron microscopy revealed mycoplasma-like organisms present in the phloem sieve elements of a big-bud tomato plant and purple top wilt potato plants infected by grafting or leafhoppers. When tubers from graft-infected potato plants were planted, 73% produced spindly shoots and 44% of these later developed purple top wilt symptoms. When scions from either field-infected or experimentally infected potato plants showing purple top wilt symptoms were grafted onto tomato plants, 24% and 62% respectively developed big-bud symptoms. The results provide strong evidence for the mycoplasmal aetiology of some, if not all, potato purple top wilt in Queensland.

scholarly journals Rapid diagnostic thin section electron microscopy of bacterial endospores

2007 ◽  
Vol 70 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Michael Laue ◽  
Bärbel Niederwöhrmeier ◽  
Norbert Bannert
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Bacterial Endospores ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

scholarly journals Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy

Nucleus ◽  
2014 ◽  
Vol 5 (6) ◽  
pp. 601-612 ◽  
Author(s):  
Vincent Duheron ◽  
Guillaume Chatel ◽  
Ursula Sauder ◽  
Vesna Oliveri ◽  
Birthe Fahrenkrog
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Structural Characterization ◽  
Human Cells ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

‘Lipidic particle’ systems as visualized by thin-section electron microscopy

1985 ◽  
Vol 812 (2) ◽  
pp. 591-594 ◽  
Author(s):  
A.J. Verkleij ◽  
B. Humbel ◽  
D. Studer ◽  
M. Müller
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Particle Systems ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

Thin-Section Electron Microscopy of Mature Myxosoma cerebralis (Myxosporidea) Spores

1975 ◽  
Vol 61 (3) ◽  
pp. 476 ◽  
Author(s):  
Philip D. Lunger ◽  
Betty L. Rhoads ◽  
Ken Wolf ◽  
Maria E. Markiw
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy
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